The Recognition of N-Glycans by the Lectin ArtinM Mediates Cell Death of a Human Myeloid Leukemia Cell Line

ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus (jackfruit), interacts with N-glycosylated receptors on the surface of several cells of hematopoietic origin, triggering cell migration, degranulation, and cytokine release. Because malignant transformation is often associated with altered expression of cell surface glycans, we evaluated the interaction of ArtinM with human myelocytic leukemia cells and investigated cellular responses to lectin binding. The intensity of ArtinM binding varied across 3 leukemia cell lines: NB4>K562>U937. The binding, which was directly related to cell growth suppression, was inhibited in the presence of Manα1-3(Manα1-6)Manβ1, and was reverted in underglycosylated NB4 cells. ArtinM interaction with NB4 cells induced cell death (IC50 = 10 µg/mL), as indicated by cell surface exposure of phosphatidylserine and disruption of mitochondrial membrane potential unassociated with caspase activation or DNA fragmentation. Moreover, ArtinM treatment of NB4 cells strongly induced reactive oxygen species generation and autophagy, as indicated by the detection of acidic vesicular organelles in the treated cells. NB4 cell death was attributed to ArtinM recognition of the trimannosyl core of N-glycans containing a ß1,6-GlcNAc branch linked to α1,6-mannose. This modification correlated with higher levels of N-acetylglucosaminyltransferase V transcripts in NB4 cells than in K562 or U937 cells. Our results provide new insights into the potential of N-glycans containing a β1,6-GlcNAc branch linked to α1,6-mannose as a novel target for anti-leukemia treatment

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Una aplicación del Teorema de Wilson

The application of the Mathematics to the resolution of problems of the daily life constitutes one of the topics that charge bigger interest in all the educational levels, it is for it that this article has as objective to expose the fundamental tools of the arithmetic to modulate, useful to understand the solution that offers to certain problems of the practice by means of the application of a resulting procedure of the Theorem of Wilson. A demonstrative example of its application is presented. In the elaboration of the work they are used as fundamental methods the analytic one synthetic and the inductive-deductive one. The proposed procedure, contrary to the theorem of Wilson, he/she gives the minimum value possible to the solution of the presented problem.La aplicación de las Matemáticas a la resolución de problemas de la vida cotidiana constituye uno de los temas que cobran mayor interés en todos los niveles educacionales, es por ello que este artículo tiene como objetivo exponer las herramientas fundamentales de la aritmética modular, útiles para comprender la solución que se ofrece a determinados problemas de la práctica mediante la aplicación de un procedimiento resultante del Teorema de Wilson. Se presenta un ejemplo demostrativo de su aplicación. En la elaboración del trabajo se utilizan como métodos fundamentales el analítico sintético y el inductivo-deductivo. El procedimiento propuesto, a diferencia del teorema de Wilson, da el valor mínimo posible a la solución del problema presentado

Una marca de agua frágil en el dominio de los momentos ortogonales de Krawtchouk

In this contribution, we propose a fragile watermarking scheme based on Krawtchouk orthogonal moments. The proposed algorithm inserts the watermarking generated in the first eight coefficients of the Krawtchouk moments, calculated from the covered image, in order to guarantee the integrity and authenticity of the emitter source. Additionally, using a private key, a public key and the Hash‐Sha256 cryptographic function, a fragile watermark is generated and will be embedded in the original image. The experimental work on the validation of the proposed scheme consists of calculating the signal‐to‐noise ratio (PSNR) and detecting manipulation of the watermarked image.En esta contribución, proponemos un esquema de marca de agua frágil basada en los momentos ortogonales de Krawtchouk. El algoritmo propuesto inserta la marca de agua generada en los primeros ocho coeficientes de los momentos de Krawtchouk, calculados a partir de la imagen cubierta, con el propósito de garantizar la integridad y autenticidad de la fuente emisora. Además, mediante una clave privada, una clave pública y la función criptográfica Hash ‐ Sha256 se genera una marca de agua frágil que será incrustada en la imagen original. El trabajo experimental sobre la validación del esquema propuesto consiste en el cálculo de la relación señal / ruido pico (PSNR) y en la detección de manipulación de la imagen marcada

“q-analogue of the apéry’s constant”

In this paper, we give a summary introduction to the Riemann zeta function. We also provide a brief overview of the q-calculus topics which are necessary to understand the main results. Finally, we give some q-representations for the q-analogue of the Apéry’s constant.En este artículo damos un resumen introductorio de la función Zeta de Riemann. También proporcionamos una breve visión de la temática  q- cálculos la cual es necesaria para un entendimiento de los principales resultados. Finalmente, damos algunas representaciones para los q-análogos de la constante Apéry´s

Representaciones hipergeométricas de algunos polinomios modificados o de tipo Sobolev

In this work we show the hypergeometric representation of some modified orthogonal polynomials and another of Sobolev type. Finally, we find the hypergeometric representation of the Laguerre-Sobolev type orthogonal polynomials.En este trabajo mostramos la representación hipergeométrica de algunos polinomios modificados y otros de tipo Sobolev. Finalmente, encontramos la representa- ción hipergeométrica de los polinomios de Laguerre de tipo Sobolev

Algunas representaciones en series de la función zeta de Riemann en argumentos impares

In this article we present some series representations of the Riemann zeta function at odd arguments proposed by several authors.En este artículo se muestran algunas representaciones en series de la función zeta de Riemann en argumentos impares, propuestas por varios autores

Los números metálicos y su vínculo con la función zeta de Riemann

In this article a brief introduction to metallic numbers is made. Next, these numbers are linked to the values of the Riemann zeta function at integer arguments.En este artículo se expone una breve introducción a los números metálicos. Luego, se vinculan estos números con los valores de la función zeta de Riemann en argumentos enteros

Latin America: situation and preparedness facing the multi-country human monkeypox outbreak

Fundación Universitaria Autónoma de las Américas. Faculty of Medicine. Grupo de Investigación Biomedicina. Pereira, Risaralda, Colombia / Universidad Científica del Sur. Master of Clinical Epidemiology and Biostatistics. Lima, Peru / Latin American network of Monkeypox Virus Research. Pereira, Risaralda, ColombiaUniversity of Buenos Aires. Cátedra de Enfermedades Infecciosas. Buenos Aires, Argentina.Hospital Britanico de Buenos Aires. Servicio de Infectología. Buenos Aires, Argentina.University of Buenos Aires. Cátedra de Enfermedades Infecciosas. Buenos Aires, Argentina / Hospital de Enfermedades Infecciosas F. J. Muniz. Buenos Aires, Argentina.University of Buenos Aires. Cátedra de Enfermedades Infecciosas. Buenos Aires, Argentina / Hospital de Enfermedades Infecciosas F. J. Muniz. Buenos Aires, Argentina.Hospital Clínico Viedma. Cochabamba, Bolivia.Gobierno Autonomo Municipal de Cochabamba. Secretaría de Salud. Centros de Salud de Primer Nivel. Direction. Cochabamba, Bolivia.Franz Tamayo University. National Research Coordination. La Paz, Bolivia.Paulista State University Júlio de Mesquita Filho. Botucatu Medical School. Infectious Diseases Department. São Paulo, SP, Brazil / Brazilian Society for Infectious Diseases. Sãao Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia. São Paulo, SP, Brazil.Institute of Infectious Diseases Emilio Ribas. São Paulo, Brazil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Centro de Referencia de Salud Dr. Salvador Allende Gossens. Policlínico Neurología. Unidad Procedimientos. Santiago de Chile, Chile.Pontificia Universidad Católica de Chile. School of Medicine. Department of Pediatric Infectious Diseases and Immunology. Santiago de Chile, Chile.Universidad Austral de Chile. Facultad de Medicina. Instituto de Salud Publica. Valdivia, Chile.Ministerio de Salud. Hospital de San Fernando. San Fernando, VI Region, Chile.Fundación Universitaria Autónoma de las Américas. Faculty of Medicine. Grupo de Investigación Biomedicina. Pereira, Risaralda, Colombia.Universidad Nacional de Colombia. Department of Pediatrics. Bogota, DC, Colombia / Hospital Pediatrico La Misericordia. Division of Infectious Diseases. Bogota, DC, Colombia.Hemera Unidad de Infectología IPS SAS. Bogota, Colombia.Hospital San Vicente Fundacion. Rionegro, Antioquia, Colombia.Clinica Imbanaco Grupo Quironsalud. Cali, Colombia / Universidad Santiago de Cali. Cali, Colombia / Clinica de Occidente. Cali, Colombia / Clinica Sebastian de Belalcazar. Valle del Cauca, Colombia.National Institute of Gastroenterology. Epidemiology Unit. La Habana, CubaHospital Salvador Bienvenido Gautier. Santo Domingo, Dominican Republic.Pontificia Universidad Catolica Madre y Maestra. Santiago, Dominican Republic.International University of Ecuador. School of Medicine. Quito, Ecuador.Universidad Tecnica de Ambato. Ambato, Ecuador.Hospital Roosevelt. Guatemala City, Guatemala.Universidad Nacional Autonoma de Honduras. Faculty of Medical Sciences. School of Medical. Unit of Scientific Research. Tegucigalpa, Honduras.Hospital Infantil de Mexico. Federico Gomez, Mexico City, Mexico.Hospital General de Tijuana. Departamento de Infectología. Tijuana, Mexico.Hospital General de Tijuana. Departamento de Infectología. Tijuana, Mexico.Asociacion de Microbiólogos y Químicos Clínicos de Nicaragua. Managua, Nicaragua.Hospital Santo Tomas. Medicine Department-Infectious Diseases Service. Panama City, Panama / Instituto Oncologico Nacional. Panama city, Panama.University of Arizona College of Medicine-Phoenix. Division of Endocrinology. Department of Medicine. Phoenix, AZ, USA / Indian School Rd. Phoenix, AZ, USA.Dirección Nacional de Vigilancia Sanitaria. Dirección de Investigación. Asunción, Paraguay.Universidad Nacional de Asuncion. Faculty of Medical Sciences. Division of Dermatology. Asuncion, Paraguay.Instituto Nacional de Salud del Nino San Borja. Infectious Diseases Division. Lima, Peru / Universidad Privada de Tacna. Facultad de Ciencias de la Salud. Tacna, Peru.Universidad San Juan Bautista. Lima, Peru.Universidad San Ignacio de Loyola. Vicerrectorado de Investigación. Unidad de Investigación para la Generación y Síntesis de Evidencias en Salud. Lima, Peru.Hospital Evangelico de Montevideo. Montevideo, Uruguay.Icahn School of Medicine at Mount Sinai. Molecular and Cell-based Medicine. Department of Pathology. Molecular Microbiology Laboratory. New York, USA / Universidad del Rosario. Facultad de Ciencias Naturales. Centro de Investigaciones en Microbiología y Biotecnología-UR. Bogota, Colombia.Hospital Evangélico de Montevideo. Montevideo, Uruguay / Venezuelan Science Incubator and the Zoonosis and Emerging Pathogens Regional Collaborative Network. Infectious Diseases Research Branch. Cabudare, Lara, Venezuela.Universidad Central de Venezuela. Faculty of Medicine. Caracas, Venezuela.Universidad Central de Venezuela. Faculty of Medicine. Caracas, Venezuela / Biomedical Research and Therapeutic Vaccines Institute. Ciudad Bolivar, Venezuela.Universidad Central de Venezuela. Tropical Medicine Institute, Infectious Diseases Section. Caracas, Venezuela.Instituto Conmemorativo Gorgas de Estudios de la Salud. Clinical Research Department. Investigador SNI Senacyt Panama. Panama City, Panama