DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF IMPURITIES FROM OLMESARTAN MEDOXIMIL AND HYDROCHLOROTHIAZIDE TABLET

Objective: To develop and validate stability indicating RP-HPLC gradient method for simultaneous estimation of impurities and degradation products from Olmesartan Medoximil and Hydrochlorothiazide tablet.Methods: The chromatographic separation was achieved by using Inertsil ODS (250 mm x 4.6 mm, 5μ) column. The mobile phase-A consists of 0.01M potassium dihydrogen phosphatebuffer pH 3.2 adjusted using orthophosphoric acids and acetonitrile as mobile phase-B. The flow rate was 1 ml/min, and chromatograms extracted at wavelength 225 nm.Results: The method was found linear from LOQ to 0.4% level with respect to target concentrations of Olmesartan Medoximil (1.6 mg/ml) and Hydrochlorothiazide (0.5 m/ml) for all impurities, with correlation coefficient found greater than 0.99. The method found robust in all deliberate variations of method parameters as a resolution between adjacent peaks found greater than 2.0. The % RSD results for precision and intermediate precision found less than 5.0%.Conclusion: The proposed analytical method was found to be robust, stability indicating and can be used for estimation of impurities and degradation products of Olmesartan Medoximil and Hydrochlorothiazide from tablet dosage form.Keywords: Stability indicating, RP-HPLC, ICH, Olmesartan Medoximil and Hydrochlorothiazid

A Supersymmetric Stueckelberg U(1) Extension of the MSSM

A Stueckelberg extension of the MSSM with only one abelian vector and one chiral superfield as an alternative to an abelian extension with Higgs scalars is presented. The bosonic sector contains a new gauge boson Z' which is a sharp resonance, and a new CP-even scalar, which combines with the MSSM Higgs bosons to produce three neutral CP-even massive states. The neutral fermionic sector has two additional fermions which mix with the four MSSM neutralinos to produce an extended 6x6 neutralino mass matrix. For the case when the LSP is composed mostly of the Stueckelberg fermions, the LSP of the MSSM will be unstable, which leads to exotic decays of sparticles with many leptons in final states. Prospects for supersymmetry searches and for dark matter are discussed.Comment: 10 page

Working Group Report: Neutrino and Astroparticle Physics

This is the report of neutrino and astroparticle physics working group at WHEPP-8. We present the discussions carried out during the workshop on selected topics in the above fields and also indicate progress made subsequently. The neutrino physics subgroup studied the possibilites of constraining neutrino masses, mixing and CPT violation in lepton sector from future experiments. Neutrino mass models in the context of abelian horizontal symmetries, warped extra dimensions and in presence of triplet Higgs were studied. Effect of threshold corrections on radiative magnification of mixing angles was investigated. The astroparticle physics subgroup focused on how various particle physics inputs affect the CMBR fluctuation spectrum, and on brane cosmology. This report also contains an introduction on how to use the publicly available code CMBFAST to calculate the CMBR fluctuations.Comment: Prepared for the 8th Workshop on High-Energy Physics Phenomenology (WHEPP-8), IIT Mumbai, India, 5-16 Jan 200

Back reaction, covariant anomaly and effective action

In the presence of back reaction, we first produce the one-loop corrections for the event horizon and Hawking temperature of the Reissner-Nordstr\"om black hole. Then, based on the covariant anomaly cancelation method and the effective action technique, the modified expressions for the fluxes of gauge current and energy momentum tensor, due to the effect of back reaction, are obtained. The results are consistent with the Hawking fluxes of a (1+1)-dimensional blackbody at the temperature with quantum corrections, thus confirming the robustness of the covariant anomaly cancelation method and the effective action technique for black holes with back reaction.Comment: 17 page

A novel diffuse gastric cancer susceptibility variant in E-cadherin (CDH1) intron 2: A case control study in an Italian population

<p>Abstract</p> <p>Background</p> <p>Inherited genetic factors such as E-cadherin (<it>CDH1</it>) promoter variants are believed to influence the risk towards sporadic diffuse gastric cancer (DGC). Recently, a new regulatory region essential for <it>CDH1 </it>transcription has been identified in <it>CDH1 </it>intron 2.</p> <p>Methods</p> <p>We genotyped all known polymorphisms located within conserved sequences of <it>CDH1 </it>intron 2 (rs10673765, rs9932686, rs1125557, rs9282650, rs9931853) in an Italian population consisting of 134 DGC cases and 100 healthy controls (55 patient relatives and 45 unrelated, matched individuals). The influence of individual variants on DGC risk was assessed using χ²-tests and logistic regression. The relative contribution of alleles was estimated by haplotype analysis.</p> <p>Results</p> <p>We observed a significant (p < 0.0004) association of the <it>CDH1 </it>163+37235G>A variant (rs1125557) with DGC risk. Odds ratios were 4.55 (95%CI = 2.09–9.93) and 1.38 (95%CI = 0.75–2.55) for AA and GA carriers, respectively. When adjusted for age, sex, smoking status, alcohol intake and <it>H. pylori </it>infection, the risk estimates remained largely significant for AA carriers. Haplotype analysis suggested the 163+37235A-allele contributes to disease risk independently of the other variants studied.</p> <p>Conclusion</p> <p>The <it>CDH1 </it>163+37235G>A polymorphism may represent a novel susceptibility variant for sporadic DGC if confirmed in other populations. Considering the broad expression of E-cadherin in epithelia, this exploratory study encourages further evaluation of the 163+37235A-allele as a susceptibility variant in other carcinomas.</p

Magnetotransport of CeRhIn5

We report measurements of the temperature-dependent anisotropic resistivity and in-plane magnetoresistance on single crystals of the tetragonal heavy-fermion antiferromagnet (TN = 3.8 K) CeRhIn5. The measurements are reported in the temperature range 1.4 K to 300 K and in magnetic fields to 18 tesla. The resistivity is moderately anisotropic, with a room-temperature c-axis to in-plane resistivity ratio rho_c/rho_a(300 K) = 1.7. rho(T) measurements on the non-magnetic analog LaRhIn5 indicate that the anisotropy in the CeRhIn5 resistivity stems predominately from anisotropy in Kondo-derived magnetic scattering. In the magnetically ordered regime an applied field H reduces TN only slightly due to the small ordered moment (0.37mu_B) and magnetic anisotropy. The magnetoresistance (MR) below TN is positive and varies linearly with H. In the paramagnetic state a positive MR is present below 7.5 K, while a high-field negative contribution is evident at higher temperatures. The positive contribution decreases in magnitude with increasing temperature. Above 40 K the positive contribution is no longer observable, and the MR is negative. The low-T positive MR results from interactions with the Kondo-coherent state, while the high-T negative MR stems from single-impurity effects. The H and T-dependent magnetotransport reflects the magnetic anisotropy and Kondo interactions at play in CeRhIn5.Comment: submitted to Physical Review

Pregnancy-Related Change in pQCT and Bone Biochemistry in a Population With a Habitually Low Calcium Intake.

In pregnancy, changes in maternal calcium (Ca) economy occur to satisfy fetal Ca demand. It is unclear whether maternal mineral reserves facilitate these requirements and no data exist from sub-Saharan Africa. The aim was to determine skeletal changes with peripheral quantitative computed tomography (pQCT) and bone biochemistry between early second and third trimesters. Pregnant rural Gambians aged 18 to 45 years (n = 467) participating in a trial of antenatal nutritional supplements (ISRCTN49285450) had pQCT scans and blood collections at mean (SD) 14 (3) and 31 (1) weeks' gestation. Outcomes were pQCT: radius/tibia 4% total volumetric bone mineral density (vBMD), trabecular vBMD, total cross-sectional area (CSA), 33%/38% radius/tibia cortical vBMD, bone mineral content (BMC), total CSA; biochemistry: collagen type 1 cross-linked β-C-telopeptide (β-CTX), type 1 procollagen N-terminal (P1NP), parathyroid hormone (PTH), and 1,25(OH)2 D. Independent t tests tested whether pooled or within-group changes differed from 0. Multiple regression was performed adjusting for age. Data for change are expressed as mean (confidence interval [CI] 2.5, 97.5%). Radius trabecular vBMD, cortical vBMD, and BMC increased by 1.15 (0.55, 1.75)%, 0.41 (0.24, 0.58)%, and 0.47 (0.25, 0.69)%. Tibia total and trabecular vBMD increased by 0.34 (0.15, 0.54)% and 0.46 (0.17, 0.74)%, while tibia cortical vBMD, BMC, and cortical CSA increased by 0.35 (0.26, 0.44)%, 0.55 (0.41, 0.68)% and 0.20 (0.09, 0.31)%, respectively. CTX, PTH, and 1,25(OH)2 D increased by 23.0 (15.09, 29.29)%, 13.2 (8.44, 19.34)%, and 21.0 (17.67, 24.29)%, while P1NP decreased by 32.4 (-37.19, -28.17)%. No evidence of mobilization was observed in the peripheral skeleton. Resorption, although higher in late versus early gestation, was lower throughout pregnancy compared with non-pregnant non-lactating (NPNL) in the same community. Formation was lower in late pregnancy than in early, and below NPNL levels. This suggests a shift in the ratio of resorption to formation. Despite some evidence of change in bone metabolism, in this population, with habitually low Ca intakes, the peripheral skeleton was not mobilized as a Ca source for the fetus. © 2021 crown copyright . Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR). The article published with the permission of the Controller of HMSO and the Queen's Printer of Scotland.

A metodologia de Lamarck

Neste artigo, o método científico de Jean-Baptiste Lamarck é estudado sob o ponto de vista de seu discurso metodológico, bem como sob o ponto de vista de sua prática científica. Essa metodologia é comparada à preconizada por Condillac, assim como à dos "ideólogos" (idéologues) grupo no qual se costuma incluir o próprio Lamarck. Mostra-se que o discurso metodológico de Lamarck assemelha-se ao dos ideólogos; no entanto, sua prática científica não se coaduna com esse enfoque. Em vez de seguir uma abordagem empirista, a obra de Lamarck se fundamenta em princípios metafísicos gerais sobre a natureza. Sob o ponto de vista dos ideólogos, seu trabalho deveria ser rejeitado - o que de fato ocorreu - como um mero sistema (système) metafísico - no sentido pejorativo utilizado pelos seguidores de Condillac. No entanto, o presente artigo argumenta que esse é justamente um importante e inovador aspecto da obra de Lamarck, que permitiu a eclosão do evolucionismo moderno