Prevalencia de esofagitis eosinofílica: estudio multicéntrico en población pediátrica evaluada en 36 centros de gastroenterología de América Latina

Introducción y objetivo: La esofagitis eosinofílica es una enfermedad crónica, mediada inmunológicamente, descrita en series y publicaciones alrededor del mundo. En los últimos 20 años diversos estudios han intentado evaluar la incidencia y prevalencia de la enfermedad. El objetivo del presente trabajo es estimar la prevalencia de esofagitis eosinofílica en un grupo de niños atendidos en 36 centros de gastroenterología pediátrica de 10 países latinoamericanos. Materiales y métodos: A través de un protocolo multicéntrico, observacional y transversal se estimó la prevalencia de período de esofagitis eosinofílica entre los niños atendidos en consulta externa y sometidos a endoscopia superior diagnóstica por cualquier motivo en 36 centros de 10 países latinoamericanos durante un período de 3 meses. Resultados: Entre abril y junio de 2016 108 casos de esofagitis eosinofílica fueron evaluados. Asimismo, un promedio de 29,253 consultas ambulatorias y 4,152 endoscopias superiores de carácter diagnóstico fueron realizadas en los 36 centros participantes. La tasa de prevalencia de esofagitis eosinofílica en la población estudiada (n = 29,253) fue de 3,69 casos × 1,000 (IC 95%: 3.04 a 4.44) y entre los niños sometidos a endoscopia superior de rutina (n = 4,152) fue de 26 x 1,000 (IC 95%: 22.6 a 29.4). Conclusión: La tasa general de prevalencia de período de esofagitis eosinofílica en un grupo de niños evaluados en 36 centros latinoamericanos de gastroenterología pediátrica resultó de 3,69 × 1,000, y entre aquellos sometidos a endoscopia fue de 26 × 1,000. La prevalencia mostró una importante variabilidad entre los países y centros participantes. Este es el primer estudio de prevalencia de esofagitis eosinofílica pediátrica en Latinoamérica. Abstract: Introduction and objective: Eosinophilic esophagitis is a chronic, immune-mediated disease described in case series and publications worldwide. Over the past twenty years, the authors of different studies have attempted to evaluate its incidence and prevalence. The objetive of the present study was to estimate the prevalence of eosinophilic esophagitis in a group of children seen at 36 pediatric gastroenterology centers in ten Latin American countries. Materials and methods: A multicenter, observational, and cross-sectional study was conducted that estimated the period prevalence of eosinophilic esophagitis in children seen at outpatient consultation and that underwent diagnostic upper gastrointestinal endoscopy for any indication at 36 centers in 10 Latin American countries, within a 3-month time frame. Results: Between April and June 2016, 108 cases of eosinophilic esophagitis were evaluated. Likewise, an average of 29,253 outpatient consultations and 4,152 diagnostic upper gastrointestinal endoscopies were carried out at the 36 participating centers. The period prevalence of eosinophilic esophagitis in the population studied (n = 29,253) was 3.69 cases × 1,000 (95% CI: 3.04 to 4.44), and among the children that underwent routine upper gastrointestinal endoscopy (n = 4,152), it was 26 x 1,000 (95% CI: 22.6 to 29.4). Conclusions: The general period prevalence of eosinophilic esophagitis in a group of children evaluated at 36 Latin American pediatric gastroenterology centers was 3.69 × 1,000, and in the children that underwent endoscopy, it was 26 × 1,000. There was important prevalence variability between the participating countries and centers. The present analysis is the first study conducted on the prevalence of pediatric eosinophilic esophagitis in Latin America. Palabras clave: Esofagitis, Eosinofílica, Niños, Prevalencia, Latinoamérica, Keywords: Esophagitis, Eosinophilic, Children, Prevalence, Latin Americ

Theoretical prediction of the enantiomeric excess in asymmetric catalysis. An alignment-independent molecular interaction field based approach

The enantiomeric excess of three different asymmetric catalyses has been predicted in excellent agreement with the experiments using a 3D-QSPR approach. In particular, GRid INdependent Descriptors generated from molecular interaction fields together with a simple partial least-squares method were found to be adequate to describe the enantioselectivity induced by these metal−ligand complexes

Accuracy vs time dilemma on the prediction of NMR chemical shifts: a case study (chloropyrimidines)

The nuclear magnetic shieldings of two chloropyrimidine species have been predicted and analyzed by means of ab initio and DFT methods. The results have been compared with the experimental values and with those from other database-related approaches. These dataset-based techniques are found to be particularly valuable because of the accurate and instantaneous prediction of the 13C chemical shifts. On the other hand, only a few quantum chemistry based approaches were showed to be the most precise to predict 1H chemical shifts and to elucidate unequivocally the 1H NMR spectra of the regioisomeric mixture under study. Special emphasis was put on incorporating the solvent effect, implicitly, or explicitly. The influence of the level of theory and basis set in the predicted values has also been discussed

Can we assess innovative bio-based chemicals in their early development stage?: A comparison between early-stage and life cycle assessments

The chemical industry strives for the development of bio-based alternatives to prepare for the transition towards a sustainable biobasedeconomy. Key in this transition is ‘safe and sustainable by design’. This entails that safety and sustainability must be taken into account at the earliest possible development stages. A remaining challenge is how to assess the sustainability and safety of a new production process while it is not yet established. Assessment methods have been developed for this purpose but they do not seem to be commonly used in Research and Development (R&D) departments. The aim of this paper is to review and evaluate the available early-stage assessment methods (ESM) and ex-ante life cycle assessment (LCA). Using the case of lactic acid in a retrospective study, its different development stages were anticipated. The outcomes of implementing the selected ESMs and ex-ante LCA at the different development stages of lactic acid were compared with those of a full LCA of the real production at commercial scale. Key findings are that 1) many ESMs are often not fully or clearly described and the databases suggested are outdated; 2) since most of the methods are designed to assess chemicals in general, not specifically for bio-based chemicals, the relevant environmental themes to reflect the characteristics of bio-based chemicals are often missing; 3) in terms of toxicity impacts, the reviewed methods are often crude and not accurate in the coverage of toxicity aspects. Ex-ante LCA could play a more important role during the process design R&D phase. However, ex-ante LCA should be complemented with accessible methods to evaluate the potential toxicity impacts at the early development stage to ensure safe by design

Bioremediation of direct dyes in simulated textile effluents by a paramorphogenic form of Aspergillus oryzae

Azo dyes are extensively used for coloring textiles, paper, food, leather, drinks, pharmaceutical products, cosmetics and inks. The textile industry consumes the largest amount of azo dyes, and it is estimated that approximately 10-15% of dyes used for coloring textiles may be lost in waste streams. Almost all azo dyes are synthetic and resist biodegradation, however, they can readily be reduced by a number of chemical and biological reducing systems. Biological treatment has advantages over physical and chemical methods due to lower costs and minimal environmental effect. This research focuses on the utilization of Aspergillus oryzae to remove some types of azo dyes from aqueous solutions. The fungus, physically induced in its paramorphogenic form (called 'pellets'), was used in the dye biosorption studies with both non-autoclaved and autoclaved hyphae, at different pH values. The goals were the removal of dyes by biosorption and the decrease of their toxicity. The dyes used were Direct Red 23 and Direct Violet 51. Their spectral stability (325-700 nm) was analyzed at different pH values (2.50, 4.50 and 6.50). The best biosorptive pH value and the toxicity limit, (which is given by the lethal concentration (LC100), were then determined. Each dye showed the same spectrum at different pH values. The best biosorptive pH was 2.50, for both non-autoclaved and autoclaved hyphae of A. oryzae. The toxicity level of the dyes was determined using the Trimmed Spearman-Karber Method, with Daphnia similis in all bioassays. The Direct Violet 51 (LC100 400 mg . mL(-1)) was found to be the most toxic dye, followed by the Direct Red 23 (LC100 900 mg . mL(-1)). The toxicity bioassays for each dye have shown that it is possible to decrease the toxicity level to zero by adding a small quantity of biomass from A. oryzae in its paramorphogenic form. The autoclaved biomass had a higher biosorptive capacity for the dye than the non-autoclaved biomass. The results show that bioremediation occurs with A. oryzae in its paramorphogenic form, and it can be used as a biosorptive substrate for treatment of industrial waste water containing azo dyes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Can we assess innovative bio-based chemicals in their early development stage?: A comparison between early-stage and life cycle assessments

The chemical industry strives for the development of bio-based alternatives to prepare for the transition towards a sustainable biobasedeconomy. Key in this transition is ‘safe and sustainable by design’. This entails that safety and sustainability must be taken into account at the earliest possible development stages. A remaining challenge is how to assess the sustainability and safety of a new production process while it is not yet established. Assessment methods have been developed for this purpose but they do not seem to be commonly used in Research and Development (R&D) departments. The aim of this paper is to review and evaluate the available early-stage assessment methods (ESM) and ex-ante life cycle assessment (LCA). Using the case of lactic acid in a retrospective study, its different development stages were anticipated. The outcomes of implementing the selected ESMs and ex-ante LCA at the different development stages of lactic acid were compared with those of a full LCA of the real production at commercial scale. Key findings are that 1) many ESMs are often not fully or clearly described and the databases suggested are outdated; 2) since most of the methods are designed to assess chemicals in general, not specifically for bio-based chemicals, the relevant environmental themes to reflect the characteristics of bio-based chemicals are often missing; 3) in terms of toxicity impacts, the reviewed methods are often crude and not accurate in the coverage of toxicity aspects. Ex-ante LCA could play a more important role during the process design R&D phase. However, ex-ante LCA should be complemented with accessible methods to evaluate the potential toxicity impacts at the early development stage to ensure safe by design

Can we assess innovative bio-based chemicals in their early development stage? A comparison between early-stage and life cycle assessments

The chemical industry strives for the development of bio-based alternatives to prepare for the transition towards a sustainable biobasedeconomy. Key in this transition is ‘safe and sustainable by design’. This entails that safety and sustainability must be taken into account at the earliest possible development stages. A remaining challenge is how to assess the sustainability and safety of a new production process while it is not yet established. Assessment methods have been developed for this purpose but they do not seem to be commonly used in Research and Development (R&D) departments. The aim of this paper is to review and evaluate the available early-stage assessment methods (ESM) and ex-ante life cycle assessment (LCA). Using the case of lactic acid in a retrospective study, its different development stages were anticipated. The outcomes of implementing the selected ESMs and ex-ante LCA at the different development stages of lactic acid were compared with those of a full LCA of the real production at commercial scale. Key findings are that 1) many ESMs are often not fully or clearly described and the databases suggested are outdated; 2) since most of the methods are designed to assess chemicals in general, not specifically for bio-based chemicals, the relevant environmental themes to reflect the characteristics of bio-based chemicals are often missing; 3) in terms of toxicity impacts, the reviewed methods are often crude and not accurate in the coverage of toxicity aspects. Ex-ante LCA could play a more important role during the process design R&D phase. However, ex-ante LCA should be complemented with accessible methods to evaluate the potential toxicity impacts at the early development stage to ensure safe by design