7 research outputs found
Bioactive Phytochemicals: Efficient Synthesis of Optically Active Substituted Flav-3-enes and Flav-3-en-3-o-R Derivatives
Published ArticleThe structural core of flavene (2-phenyl-2H-chromene) is commonly found in plant flavonoids, which exhibit a wide range of
biological activities and diverse pharmacological profiles (e.g., antioxidant and anticancer activities). Flavonoids have attracted
significant interest inmedicinal and synthetic chemistry. Substituted flav-3-ene 13 was exclusively synthesized by the stereoselective
elimination of the O-mesyl moiety on C-3 of 5,7,3,4-tetramethoxyflavan-3-mesylate 12 with 1,8-diazabicyclo[5.4.0]undec-7-ene.
The reaction of 5,7,3,4-tetramethoxyflavan-3-one 15 with ytterbium trifluoromethanesulfonate in methanol afforded a novel 3-Osubstituted
flav-3-ene derivative (3,5,7,3,4-pentamethoxyflav-3-ene) 17. The reduction of 4-(1,3,5-trihydroxybenzene)-5,7,3,4-
tetra-O-benzylflavan-3-one 19b with hydrogen afforded a new compound: 3-hydroxy-4-(1,3,5-trihydroxybenzene)-5,7,3,4-
tetrahydroxyflavan-3-en-3-ol 21 in good yield (95%), while the acetylation of 19a and 21 afforded the expected novel flav-3-en-
3-acetoxy derivatives 20 (92%) and 22 (90%), respectively
Salinity Effects on External and Internal Morphology of Rose Geranium (Pelargonium graveolensL.) Leaf
Published ArticleSalinity is an environmental condition that induces stress on plants especially under high soil salts levels.
It limits crop metabolic activities, hampers plant growth and synthesis of secondary metabolites. It also affects
osmotic potential in the plant root zone. A complete randomized block design was used to evaluate the effect of
salinity applied at 1.6, 2.4, 3.2 and 4.0 mS cm-1 on external and internal morphology of rose geranium (Pelargonium
graveolens L.) leaf and treatments were replicated three times. Scanning electron microscopy (SEM) and
transmission electron microscopy (TEM) were used to evaluate the morphology of the leaf. Salinity levels induced
the development of capitate trichome. Abaxial leaf position had the highest number of trichomes than the adaxial
leaf position. A strong polynomial (r2=0.97) relationship was found between capitate trichome and salinity. High
densities of capitate trichomes were found at a high salinity level. Although the development of asciiform trichome
was induced, it was in an insignificant level, trichomes densities are therefore not affected by salinity. It was
therefore concluded that rose geranium might have some degree of tolerance to salinity
Salinity Effects on External and Internal Morphology of Rose Geranium (Pelargonium graveolensL.) Leaf
Abstract: Salinity is an environmental condition that induces stress on plants especially under high soil salts levels. It limits crop metabolic activities, hampers plant growth and synthesis of secondary metabolites. It also affects osmotic potential in the plant root zone. A complete randomized block design was used to evaluate the effect of salinity applied at 1.6, 2.4, 3.2 and 4.0 mS cm -1 on external and internal morphology of rose geranium (Pelargonium graveolens L.) leaf and treatments were replicated three times. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to evaluate the morphology of the leaf. Salinity levels induced the development of capitate trichome. Abaxial leaf position had the highest number of trichomes than the adaxial leaf position. A strong polynomial (r 2 =0.97) relationship was found between capitate trichome and salinity. High densities of capitate trichomes were found at a high salinity level. Although the development of asciiform trichome was induced, it was in an insignificant level, trichomes densities are therefore not affected by salinity. It was therefore concluded that rose geranium might have some degree of tolerance to salinity
Using Biostimulants Containing Phytohormones to Recover Hail-Damaged Essential Oil Plants
Hail can cause significant damage to aromatic and medicinal plants; however, this has never been investigated scientifically on most of aromatic and medicinal plants. Globally, essential oil crop producers primarily make use of agricultural crop insurance and costly mitigation strategies to recover lost production costs and alleviate hail-damaged plants. However, most aromatic and medicinal plants are not covered under agricultural crop insurance, and many commercial farmers are not able to regularly employ expensive alternative strategies. Therefore, hail damage may present a challenge to essential oil growers. The use of natural and synthetic phytohormones in a form of biostimulants, as an alternative biological mitigation strategy against hail damage in essential oil crops, has not received much attention, and there is no information on this topic. Exogenous applications of natural and synthetic biostimulants have consistently demonstrated growth enhancement, nutrient acquisition, yield and quality optimization, as well as physiological efficiency in plants. Biostimulants in a form of phytohormones are involved in diverse plant physiological processes, including the regulation of gene expression for adaptive responses to biotic and abiotic stresses. Using biostimulants, this chapter will detail the potential recovery response of aromatic and medicinal plants to hail damage, and the response of plants treated with biostimulants
Optimising water use efficiency for crop production
Thesis (MScAgric)--University of Stellenbosch, 2003.ENGLISH ABSTRACT: Poor water management and poor water use efficiency (WUE) have been identified
as one of the major problems experienced by vegetable growers in most of the
developing countries, including South Africa. This poor management and poor
utilization of water have led to a drastic decline in the quality and quantity of
available water. In South Africa agriculture uses about 50% of available water.
Increasing water demand for domestic, industrial and mining uses, may decrease
agriculture's share to less than the current 50%, henceforth, better utilization of this
resource is imperative. Selection of a good irrigation system can limit water loss
considerably. Some irrigation systems have a potential to save more water than
others do.
Since irrigation systems affect the WUE of crops, care should be taken when
selecting an irrigation system under conditions of limited water quantity. Ebb-and-
Flood watering systems have been introduced for effective sub-irrigation and
nutrient delivery within closed systems. Such a system was adapted in South Africa,
to develop a vegetable production unit for use by families in rural communities,
while saving substantial amounts of water. A need to further improve the WUE of
this system was subsequently identified. Two studies were conducted at the
experimental farm of the University of Stellenbosch (Department of Agronomy).
The first trial was conducted under controlled conditions in a glasshouse, and the
second under open field conditions.
In the first trial, Beta vulgaris (Swiss chard) and Amaranthus spp. ('Imbuya') were
grown in two root media; gravel and pumice. In addition, an 'Ebb-and-Flood' and a
'Constant level' system were used with nutrient solutions at two electrical (EC)
conductivity levels 1.80 and 3.60 mS cm-I. The results of this (2x2x2x2) factorial
experiment indicated that a combination of the 'Ebb-and-Flood' system with gravel
as a root medium produced the best results at a low EC, when 'imbuya' was used. A
high total WUE was found with 'imbuya', (7.35 g L-I) at EC 1.80 mS cmicompared
to a relatively low WUE of 5. 90 g L-I when the 3.60 mS cm-I nutrient solution was
used.
In the second trial, 'Imbuya's' foliage dry mass, leaf area and WUE was evaluated
under field conditions at the Stellenbosch University experimental farm, during the summer of2002. The experimental farm (33°55'S, 18°52'E) is situated in the cooler
coastal wine grape-producing region of South Africa with a relatively high annual
winter rainfall. This trial was conducted on an alluvial soil, with clay content of 25%
and a pH of 5.9 (KC!). A closed 'Ebb-and-Flood' system was compared with two
open field irrigation systems ('Drip' and 'Flood') using nutrient solutions at two
electrical conductivity levels (1.80 and 3.60 mS cm-i) in all three cases. Foliage dry
mass, leaf area as well as WUE was best with 'Drip' irrigation, when a nutrient
solution with an electrical conductivity of 3.60 mS cm-i was used. In spite of the
fact that additional ground water was available for the soil grown 'Drip' and 'Flood'
treatments, the 'Ebb-and-Flood' system outperformed the 'Flood' treatment,
especially when the nutrient solution with an EC of 3.6 mS cm-i was used.
Insufficient root aeration in the flooded soil could have been a contributing factor.
The fact that the 'Ebb-and-Flood' and 'Drip' systems gave the best results when the
high EC solution was used to fertigate the plants, may indicate that the plants could
have hardened due to the mild EC stress, better preparing them to adapt to the
extreme heat that was experienced in the field.AFRIKAANSE OPSOMMING: Swak: bestuur van water en 'n swak: water-gebruik-doeltreffendheid (WOD) is as een
van die belangrikste probleme geïdentifiseer wat deur groente produsente in die
meeste ontwikkelende lande, insluitend Suid-Afrika, ervaar word. Hierdie swak
bestuur en benutting van water het daartoe bygedra dat 'n drastiese afname in die
kwaliteit asook in die kwantiteit van beskikbare water ervaar word. In Suid-Afrika
gebruik die landbou-sektor ongeveer 50% van die beskikbare water. Toenemende
water behoeftes vir huisgebruik, industrieë en die mynbou mag hierdie 50% aandeel
van die landbou sektor laat krimp. Beter benutting van hierdie skaars hulpbron is
dus noodsaaklik. Die keuse van goeie besproeiingsisteme mag waterverliese
merkbaar beperk aangesien sekere sisteme se water-besparingspotensiaal beter as
ander is.
Aangesien besproeiingstelsels die WOD van gewasse beïnvloed, is spesiale sorg
nodig waar 'n besproeiingstelsel onder hierdie toestande van beperkte waterbronne
gekies moet word. 'Ebb-en-Vloed' sisteme kan aangewend word om water en
voedingselemente van onder in 'n wortelmedium te laat opstoot en in 'n geslote
sisteem te laat terugdreineer. So 'n sisteem is in Suid-Afrika ontwikkel waarmee
groente vir families in landelike gebiede geproduseer kan word terwyl water bespaar
word. 'n Behoefte om die WOD van hierdie produksiesisteem verder te verbeter is
egter geïdentifiseer. Twee ondersoeke is by die Universiteit van Stellenbosch se
proefplaas (Departement Agronomie) gedoen. Die eerste proef is onder beheerde
omgewingstoestande in 'n glashuis uitgevoer en die tweede onder veld toestande.
In die eerste proef is Beta vulgaris (Snybeet) en Amaranthus spp. ('Imbuya') in
twee tipes wortelmedia; gruis en puimsteen verbou. 'n 'Ebb-en-Vloed' asoook 'n
'Konstante vlak' besproeiingsisteem is gebruik terwyl voedingsoplossings ook by
twee peile van elektriese geleiding (EC) teen 1.80 en 3.60 mS cm-I toegedien is. Die
resultate van hierdie (2x2x2x2) fakroriaal eksperiment het aangetoon dat 'n
kombinasie van die 'Ebb-en-Vloed' sisteem met gruis as 'n wortelmedium die beste
resultate teen 'n lae EC lewer waar 'imbuya' gebruik is. Die WOD met 'imbuya'
was hoog (7.35 g L-1) by 'n EC van 1.80 mS cm-I, vergeleke met 'n relatief lae
WOD van 5. 90 g L-1 waar die 3.60 mS cm-I voedingsoplossing gebruik is.
In die tweede proef is 'Imbuya' se droë blaarmassa, blaar oppervlakte en WOD
onder veldtoestande op die Universiteit van Stellenbsoch se proefplaas in die somer van 2002 ge-evalueer. Die proefplaas (33°55'S, 18°52'E) is in die koeler kusstreke
van die wyndruif produksiegebied in die winterreëngebied van Suid-Afrika geleë.
Hierdie proef is op alluviale grond met 25% klei en 'n pH van 5.9 (KCi) uitgevoer.
'n Geslote 'Ebb-en-Vloed' sisteem is met twee veld-besproeiingsisteme vergelyk
('Drup' en 'Vloed') terwyl voedingsoplossings teen twee peile van elektriese
geleiding (1.80 en 3.60 mS cm-I) in al drie gevalle gebruik is. Blaar droëmassa,
blaaroppervlakte asook die WGD was die beste met 'Drup' besproeiing waar die EC
van die voedingsoplossing 3.60 mS cm-I was. Ten spyte van die feit dat ekstra
grondwater vir die 'Drup' and 'Vloed' behandelings beskikbaar was, het die 'Ebben-
Vloed' stelsel beter as die 'Vloed' behandeling gedoen veral waar die
voedingsoplossing se EC 3.6 mS cm-I was. Swak wortelbelugting was waarskynlik
die rede waarom vloedbesproeiing swak produksie gelewer het. Die feit dat die
'Drup' en die 'Ebb-en-Vloed' behandelings in die veldproef die beste gedoen het
waar die EC hoog was, mag moontlik met die gehardheid van die plante verband hou
wat aan ekstreem warm en dor toestande blootgestel was
Probiotic Potential, Iron and Zinc Bioaccessibility, and Sensory Quality of Uapaca kirkiana Fruit Jam Fermented with Lactobacillus rhamnosus Yoba
Uapaca kirkiana is an underutilised indigenous fruit tree (IFT) found in the miombo ecological zone in sub-Saharan Africa. Furthermore, sub-Saharan Africa is home to many nutritionally insecure people who suffer from micronutrient deficiency. The incorporation of probiotic strains in jams as a possible way of enhancing mineral accessibility, food quality, and health is limited in Africa. This study monitored the probiotic potential, bioaccessible iron and zinc, and organoleptic properties of U. kirkiana fruit jam fermented with L. rhamnosus yoba. U. kirkiana fruits were collected from semiarid rural areas of Zimbabwe. The L. rhamnosus yoba strain was obtained from the Yoba for Life Foundation, Netherlands. Mineral and biochemical properties of the probiotic jam were analysed using AOAC standard methods. The U. kirkiana fruit tree was ranked first as a food resource by most rural populations in Zimbabwe. The probiotic jam formulation had 55% (wt/vol) U. kirkiana fruit pulp, 43% (wt/vol) sugar, 1.25% (wt/vol) pectin, 0.5% (wt/vol) citric acid, and 0.25% (wt/vol) L. rhamnosus yoba strain. The probiotic jam had 6.2±0.2 log CFU/mL viable L. rhamnosus yoba cells. Iron and zinc content (mg/100 g w.b.) was 4.13±0.22 and 0.68±0.02 with pH 3.45±0.11, respectively. Nutrient content was g/100 g w.b., carbohydrate 66±4.1, fat 0.1±0.01, crude protein 0.2±0.01, ash 0.7±0.02, and crude fiber 0.3±0.01. Bioaccessible iron and zinc were 6.55±0.36% and 16.1±0.50% and increased by 4% and 2% in the probiotic jam, respectively. Mineral bioaccessibility and nutrient content were significantly different (p<0.05) in jam with 0.25% L. rhamnosus yoba. Jam acceptance rating was 83%. The probiotic jam can be used as a sustainable food containing probiotic with potential nutritional and health benefits
Diversity analysis of Artemisia afra L. (wormwood) accessions using diversity arrays technology and foliar mineral content attributes
The objectives of this study were to evaluate and characterized A. afra accessions using Diversity Arrays Technology and foliar plant minerals concentration. A total of 80 samples were collected from the localities of Roma and Mohale’s Hoek in Lesotho and Wepener and Hobhouse in the Free State province of South Africa. A dendrogram was used to classify accessions into 3 clusters, which were further divided into 7 subclusters. The heights and clades were dissimilar and ranged from 53.1 to 100 bp. A principal component analysis supported the results from an unweighted pair group method with arithmetic mean analysis by classifying eigenvalues into 3 groups. Most acces-sions were grouped together regardless of their origin. Moreover, a minimum spanning network analysis showed close relationships for most accessions, even though they were classified in differ-ent groups. Among the populations of A. afra, heterozygosity was due to the complete segregation of genes. Substantial variation among genotypes was uncovered by an analysis of variance con-ducted on foliar minerals. Foliar nitrogen, potassium, calcium, magnesium, and manganese were significantly affected by collection location. However, A. afra obtained in locales Roma and Mohale’s Hoek, were genetically diverse and differed in foliar mineral contents.</p