Poll Workers and Polling Places

In the year after the 2000 presidential election debacle in Florida, there was a sharp focus by many organizations, commissions, and interest groups to determine how to address the problems associated with ensuring that the events of November 2000 did not occur again (e.g., Carter and Ford 2001; VTP 2001). Not surprisingly, the discussion of these entities focused strongly around two important issues. First, there was a sharp focus on the issues related to voting technologies. Given that the difficulty, if not impossibility, of determining how to count certain ballots and the intent of the voter in marking such ballots, determining how to design a voting system that addressed this problem so that it would not occur again seemed paramount. Second, it was also clear, based on the events in Florida and in other states, that many voters had never been able to get to the point of being able to mark – or mismark! – a ballot because problems with the voter registration system had not allowed them to be authenticated as valid voters. By focusing on addressing problems related to voting technology and voter registration, election reform in 2001 and 2002 attempted to address to technologies used in voting – the way people vote – and the processes that were used to address voting issues, such as allowing provisional voting when voter registration errors occurred. The Help America Vote Act (HAVA) focused most of its funding and reforms on modernizing voter registration systems by creating statewide voter registration systems and procedures for addressing problems with voter registration through provisional voting requirement and on modernizing voting systems by banning punch card voting in federal elections and requiring the adoption of new voting systems that were auditable and provided users with feedback regarding any errors they might have made in voting

How well can regional fluxes be derived from smaller-scale estimates?

Regional surface fluxes are essential lower boundary conditions for large scale numerical weather and climate models and are the elements of global budgets of important trace gases. Surface properties affecting the exchange of heat, moisture, momentum and trace gases vary with length scales from one meter to hundreds of km. A classical difficulty is that fluxes have been measured directly only at points or along lines. The process of scaling up observations limited in space and/or time to represent larger areas was done by assigning properties to surface classes and combining estimated or calculated fluxes using an area weighted average. It is not clear that a simple area weighted average is sufficient to produce the large scale from the small scale, chiefly due to the effect of internal boundary layers, nor is it known how important the uncertainty is to large scale model outcomes. Simultaneous aircraft and tower data obtained in the relatively simple terrain of the western Alaska tundra were used to determine the extent to which surface type variation can be related to fluxes of heat, moisture, and other properties. Surface type was classified as lake or land with aircraft borne infrared thermometer, and flight level heat and moisture fluxes were related to surface type. The magnitude and variety of sampling errors inherent in eddy correlation flux estimation place limits on how well any flux can be known even in simple geometries

Restoring a Culturally Eutrophic Shallow Lake: Case Study on Quamichan Lake in North Cowichan, British Columbia

Quamichan Lake is a culturally eutrophic shallow lake located in North Cowichan on Vancouver Island in British Columbia. My research project examined the current trophic status and water quality of Quamichan Lake and investigated a number of watershed and in-lake restoration methods to return the lake back to mesotrophic (nutrient rich) conditions. Based on the data collected, Quamichan Lake is currently in a hypertrophic state caused by excess phosphorus inputs that leads to Cyanophyte phytoplankton species (cyanobacteria) to dominate during the summer. Eutrophication is both an environmental and human health issue as cyanobacteria algal blooms can disrupt the lake ecology and are toxic to most mammals. The goal of my research was to provide the Municipality of North Cowichan and Vancouver Island Health Authority with a comprehensive restoration plan to contribute to the restoration of Quamichan Lake and other lakes in southern Vancouver Island that are experiencing cultural eutrophication

Daytime turbulent exchange between the Amazon forest and the atmosphere

Detailed observations of turbulence just above and below the crown of the Amazon rain forest during the wet season are presented. The forest canopy is shown to remove high frequency turbulent fluctuations while passing lower frequencies. Filter characteristics of turbulent transfer into the Amazon rain forest canopy are quantified. Simple empirical relations that relate observed turbulent heat fluxes to horizontal wind variance are presented. Changes in the amount of turbulent coupling between the forest and the boundary layer associated with deep convective clouds are presented both as statistical averages and as a series of case studies. These convective processes during the rainy season are shown to alter the diurnal course of turbulent fluxes. In wake of giant coastal systems, no significant heat or moisture fluxes occur for up to a day after the event. Radar data is used to demonstrate that even small raining clouds are capable of evacuating the canopy of substances normally trapped by persistent static stability near the forest floor. Recovery from these events can take more than an hour, even during mid-day. In spite of the ubiquitous presence of clouds and frequent rain during this season, the average horizontal wind speed spectrum is well described by dry CBL similarity hypotheses originally found to apply in flat terrain

Multi-year longitudinal profiles of cortisol and corticosterone recovered from baleen of North Atlantic right whales (Eubalaena glacialis)

© The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in General and Comparative Endocrinology 254 (2017): 50-59, doi:10.1016/j.ygcen.2017.09.009.Research into stress physiology of mysticete whales has been hampered by difficulty in obtaining repeated physiological samples from individuals over time. We investigated whether multi-year longitudinal records of glucocorticoids can be reconstructed from serial sampling along full-length baleen plates (representing ~10 years of baleen growth), using baleen recovered from two female North Atlantic right whales (Eubalaena glacialis) of known reproductive history. Cortisol and corticosterone were quantified with immunoassay of subsamples taken every 4 cm (representing ~60 d time intervals) along a full-length baleen plate from each female. In both whales, corticosterone was significantly elevated during known pregnancies (inferred from calf sightings and necropsy data) as compared to intercalving intervals; cortisol was significantly elevated during pregnancies in one female but not the other. Within intercalving intervals, corticosterone was significantly elevated during the first year (lactation year) and/or the second year (post-lactation year) as compared to later years of the intercalving interval, while cortisol showed more variable patterns. Cortisol occasionally showed brief high elevations (“spikes”) not paralleled by corticosterone, suggesting that the two glucocorticoids might be differentially responsive to certain stressors. Generally, immunoreactive corticosterone was present in higher concentration in baleen than immunoreactive cortisol; corticosterone:cortisol ratio was usually >4 and was highly variable in both individuals. Further investigation of baleen cortisol and corticosterone profiles could prove fruitful for elucidating long-term, multi-year patterns in stress physiology of large whales, determined retrospectively from stranded or archived specimens.This work was supported by the Eppley Foundation for Research, the NOAA Marine Mammal Health and Stranding Program, the Woods Hole Oceanographic Institution Ocean Life Institute, and the New England Aquarium

Longitudinal progesterone profiles in baleen from female North Atlantic right whales (Eubalaena glacialis) match known calving history

© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Conservation Physiology 4 (2016): cow014, doi:10.1093/conphys/cow014.Reproduction of mysticete whales is difficult to monitor, and basic parameters, such as pregnancy rate and inter-calving interval, remain unknown for many populations. We hypothesized that baleen plates (keratinous strips that grow downward from the palate of mysticete whales) might record previous pregnancies, in the form of high-progesterone regions in the sections of baleen that grew while the whale was pregnant. To test this hypothesis, longitudinal baleen progesterone profiles from two adult female North Atlantic right whales (Eubalaena glacialis) that died as a result of ship strike were compared with dates of known pregnancies inferred from calf sightings and post-mortem data. We sampled a full-length baleen plate from each female at 4 cm intervals from base (newest baleen) to tip (oldest baleen), each interval representing ∼60 days of baleen growth, with high-progesterone areas then sampled at 2 or 1 cm intervals. Pulverized baleen powder was assayed for progesterone using enzyme immunoassay. The date of growth of each sampling location on the baleen plate was estimated based on the distance from the base of the plate and baleen growth rates derived from annual cycles of stable isotope ratios. Baleen progesterone profiles from both whales showed dramatic elevations (two orders of magnitude higher than baseline) in areas corresponding to known pregnancies. Baleen hormone analysis shows great potential for estimation of recent reproductive history, inter-calving interval and general reproductive biology in this species and, possibly, in other mysticete whales.This work was supported by the Eppley Foundation for Research, the National Oceanographic and Atmospheric Administration Marine Mammal Health and Stranding Program and the Woods Hole Oceanographic Institution Ocean Life Institute

Ontogenetic changes in skeletal muscle fiber type, fiber diameter and myoglobin concentration in the Northern elephant seal (Mirounga angustirostris)

© The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Physiology 5 (2014): 217, doi:10.3389/fphys.2014.00217.Northern elephant seals (Mirounga angustirostris) (NES) are known to be deep, long-duration divers and to sustain long-repeated patterns of breath-hold, or apnea. Some phocid dives remain within the bounds of aerobic metabolism, accompanied by physiological responses inducing lung compression, bradycardia, and peripheral vasoconstriction. Current data suggest an absence of type IIb fibers in pinniped locomotory musculature. To date, no fiber type data exist for NES, a consummate deep diver. In this study, NES were biopsied in the wild. Ontogenetic changes in skeletal muscle were revealed through succinate dehydrogenase (SDH) based fiber typing. Results indicated a predominance of uniformly shaped, large type I fibers and elevated myoglobin (Mb) concentrations in the longissimus dorsi (LD) muscle of adults. No type II muscle fibers were detected in any adult sampled. This was in contrast to the juvenile animals that demonstrated type II myosin in Western Blot analysis, indicative of an ontogenetic change in skeletal muscle with maturation. These data support previous hypotheses that the absence of type II fibers indicates reliance on aerobic metabolism during dives, as well as a depressed metabolic rate and low energy locomotion. We also suggest that the lack of type IIb fibers (adults) may provide a protection against ischemia reperfusion (IR) injury in vasoconstricted peripheral skeletal muscle.Funding was provided by the Baylor University Faculty Research Investment Program (StephenJ.Trumble)

Multiple steroid and thyroid hormones detected in baleen from eight whale species

© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Conservation Physiology 5 (2017): cox061, doi:10.1093/conphys/cox061.Recent studies have demonstrated that some hormones are present in baleen powder from bowhead (Balaena mysticetus) and North Atlantic right (Eubalaena glacialis) whales. To test the potential generalizability of this technique for studies of stress and reproduction in large whales, we sought to determine whether all major classes of steroid and thyroid hormones are detectable in baleen, and whether these hormones are detectable in other mysticetes. Powdered baleen samples were recovered from single specimens of North Atlantic right, bowhead, blue (Balaenoptera [B.]musculus), sei (B. borealis), minke (B. acutorostrata), fin (B. physalus), humpback (Megaptera novaeangliae) and gray (Eschrichtius robustus) whales. Hormones were extracted with a methanol vortex method, after which we tested all species with commercial enzyme immunoassays (EIAs, Arbor Assays) for progesterone, testosterone, 17β-estradiol, cortisol, corticosterone, aldosterone, thyroxine and tri-iodothyronine, representing a wide array of steroid and thyroid hormones of interest for whale physiology research. In total, 64 parallelism tests (8 species × 8 hormones) were evaluated to verify good binding affinity of the assay antibodies to hormones in baleen. We also tested assay accuracy, although available sample volume limited this test to progesterone, testosterone and cortisol. All tested hormones were detectable in baleen powder of all species, and all assays passed parallelism and accuracy tests. Although only single individuals were tested, the consistent detectability of all hormones in all species indicates that baleen hormone analysis is likely applicable to a broad range of mysticetes, and that the EIA kits tested here perform well with baleen extract. Quantification of hormones in baleen may be a suitable technique with which to explore questions that have historically been difficult to address in large whales, including pregnancy and inter-calving interval, age of sexual maturation, timing and duration of seasonal reproductive cycles, adrenal physiology and metabolic rate.This work was supported by (1) the Center for Bioengineering Innovation at Northern Arizona University and (2) the New England Aquarium

Fecal glucocorticoids and anthropogenic injury and mortality in North Atlantic right whales Eubalaena glacialis

© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Endangered Species Research 34 (2017): 417-429, doi:10.3354/esr00866.As human impacts on marine ecosystems escalate, there is increasing interest in quantifying sub-lethal physiological and pathological responses of marine mammals. Glucocorticoid hormones are commonly used to assess stress responses to anthropogenic factors in wildlife. While obtaining blood samples to measure circulating hormones is not currently feasible for free-swimming large whales, immunoassay of fecal glucocorticoid metabolites (fGCs) has been validated for North Atlantic right whales Eubalaena glacialis (NARW). Using a general linear model, we compared fGC concentrations in right whales chronically entangled in fishing gear (n = 6) or live-stranded (n = 1), with right whales quickly killed by vessels (n = 5) and healthy right whales (n = 113) to characterize fGC responses to acute vs. chronic stressors. fGCs in entangled whales (mean ± SE: 1856.4 ± 1644.9 ng g-1) and the stranded whale (5740.7 ng g-1) were significantly higher than in whales killed by vessels (46.2 ± 19.2 ng g-1) and healthy whales (51.7 ± 8.7 ng g-1). Paired feces and serum collected from the live-stranded right whale provided comparison of fGCs in 2 matrices in a chronically stressed whale. Serum cortisol and corticosterone in this whale (50.0 and 29.0 ng ml-1, respectively) were much higher than values reported in other cetaceans, in concordance with extremely elevated fGCs. Meaningful patterns in fGC concentration related to acute vs. chronic impacts persisted despite potential for bacterial degradation of hormone metabolites in dead whales. These results provide biological validation for using fGCs as a biomarker of chronic stress in NARWs.This research was funded by the NOAA/NMFS, Office of Naval Research Marine Mammals and Biology Program, Northeast Consortium, Island Foundation, Irving Oil, NEAq Internal Research Fund, Prescott Grant NA08NMF4390590, and NOAA CINAR Cooperative Agreement NA09OAR4320129

Toward an understanding of risk factors for binge-eating disorder in black and white women: A community-based case-control study

This study sought to identify in white women risk factors specific to binge-eating disorder (BED) and for psychiatric disorders in general, and to compare black and white women on risk factors for BED.