88 research outputs found
Multisensory task demands temporally extend the causal requirement for visual cortex in perception
Primary sensory areas constitute crucial nodes during perceptual decision making. However, it remains unclear to what extent they mainly constitute a feedforward processing step, or rather are continuously involved in a recurrent network together with higher-order areas. We found that the temporal window in which primary visual cortex is required for the detection of identical visual stimuli was extended when task demands were increased via an additional sensory modality that had to be monitored. Late-onset optogenetic inactivation preserved bottom-up, early-onset responses which faithfully encoded stimulus features, and was effective in impairing detection only if it preceded a late, report-related phase of the cortical response. Increasing task demands were marked by longer reaction times and the effect of late optogenetic inactivation scaled with reaction time. Thus, independently of visual stimulus complexity, multisensory task demands determine the temporal requirement for ongoing sensory-related activity in V1, which overlaps with report-related activity
Divisive Normalization and Neuronal Oscillations in a Single Hierarchical Framework of Selective Visual Attention
Divisive normalization models of covert attention commonly use spike rate modulations as indicators of the effect of top-down attention. In addition, an increasing number of studies have shown that top-down attention increases the synchronization of neuronal oscillations as well, particularly in gamma-band frequencies (25–100 Hz). Although modulations of spike rate and synchronous oscillations are not mutually exclusive as mechanisms of attention, there has thus far been little effort to integrate these concepts into a single framework of attention. Here, we aim to provide such a unified framework by expanding the normalization model of attention with a multi-level hierarchical structure and a time dimension; allowing the simulation of a recently reported backward progression of attentional effects along the visual cortical hierarchy. A simple cascade of normalization models simulating different cortical areas is shown to cause signal degradation and a loss of stimulus discriminability over time. To negate this degradation and ensure stable neuronal stimulus representations, we incorporate a kind of oscillatory phase entrainment into our model that has previously been proposed as the “communication-through-coherence” (CTC) hypothesis. Our analysis shows that divisive normalization and oscillation models can complement each other in a unified account of the neural mechanisms of selective visual attention. The resulting hierarchical normalization and oscillation (HNO) model reproduces several additional spatial and temporal aspects of attentional modulation and predicts a latency effect on neuronal responses as a result of cued attention
A comparison of 3D particle, fluid and hybrid simulations for negative streamers
In the high field region at the head of a discharge streamer, the electron
energy distribution develops a long tail. In negative streamers, these
electrons can run away and contribute to energetic processes such as
terrestrial gamma-ray and electron flashes. Moreover, electron density
fluctuations can accelerate streamer branching. To track energies and locations
of single electrons in relevant regions, we have developed a 3D hybrid model
that couples a particle model in the region of high fields and low electron
densities with a fluid model in the rest of the domain. Here we validate our 3D
hybrid model on a 3D (super-)particle model for negative streamers in
overvolted gaps, and we show that it almost reaches the computational
efficiency of a 3D fluid model. We also show that the extended fluid model
approximates the particle and the hybrid model well until stochastic
fluctuations become important, while the classical fluid model underestimates
velocities and ionization densities. We compare density fluctuations and the
onset of branching between the models, and we compare the front velocities with
an analytical approximation
Positive and negative streamers in ambient air: modeling evolution and velocities
We simulate short positive and negative streamers in air at standard
temperature and pressure. They evolve in homogeneous electric fields or emerge
from needle electrodes with voltages of 10 to 20 kV. The streamer velocity at
given streamer length depends only weakly on the initial ionization seed,
except in the case of negative streamers in homogeneous fields. We characterize
the streamers by length, head radius, head charge and field enhancement. We
show that the velocity of positive streamers is mainly determined by their
radius and in quantitative agreement with recent experimental results both for
radius and velocity. The velocity of negative streamers is dominated by
electron drift in the enhanced field; in the low local fields of the present
simulations, it is little influenced by photo-ionization. Though negative
streamer fronts always move at least with the electron drift velocity in the
local field, this drift motion broadens the streamer head, decreases the field
enhancement and ultimately leads to slower propagation or even extinction of
the negative streamer.Comment: 18 pages, 10 figure
Genomic Diversity within the Enterobacter cloacae Complex
Background: Isolates of the Enterobacter cloacae complex have been increasingly isolated as nosocomial pathogens, but phenotypic identification of the E. cloacae complex is unreliable and irreproducible. Identification of species based on currently available genotyping tools is already superior to phenotypic identification, but the taxonomy of isolates belonging to this complex is cumbersome. Methodolgy/Principal Findings: This study shows that multilocus sequence analysis and comparative genomic hybridization based on a mixed genome array is a powerful method for studying species assignment within the E. cloacae complex. The E. cloacae complex is shown to be evolutionarily divided into two clades that are genetically distinct from each other. The younger first clade is genetically more homogenous, contains the Enterobacter hormaechei species and is the most frequently cultured Enterobacter species in hospitals. The second and older clade consists of several (sub)species that are genetically more heterogonous. Genetic markers were identified that could discriminate between the two clades and cluster 1. Conclusions/Significance: Based on genomic differences it is concluded that some previously defined (clonal and heterogenic) (sub)species of the E. cloacae complex have to be redefined because of disagreements with known or proposed nomenclature. However, further improved identification of the redefined species will be possible based on novel markers presented here. © 2008 Paauw et al. Chemicals / CAS: Bacterial Proteins; DNA, Bacteria
Crossmodal duration perception involves perceptual grouping, temporal ventriloquism, and variable internal clock rates
Here, we investigate how audiovisual context affects perceived event duration with experiments in which observers reported which of two stimuli they perceived as longer. Target events were visual and/or auditory and could be accompanied by nontargets in the other modality. Our results demonstrate that the temporal information conveyed by irrelevant sounds is automatically used when the brain estimates visual durations but that irrelevant visual information does not affect perceived auditory duration (Experiment 1). We further show that auditory influences on subjective visual durations occur only when the temporal characteristics of the stimuli promote perceptual grouping (Experiments 1 and 2). Placed in the context of scalar expectancy theory of time perception, our third and fourth experiments have the implication that audiovisual context can lead both to changes in the rate of an internal clock and to temporal ventriloquism-like effects on perceived on- and offsets. Finally, intramodal grouping of auditory stimuli diminished any crossmodal effects, suggesting a strong preference for intramodal over crossmodal perceptual grouping (Experiment 5)
Optimization of interneuron function by direct coupling of cell migration and axonal targeting
Neural circuit assembly relies on the precise synchronization of developmental processes, such as cell migration and axon targeting, but the cell-autonomous mechanisms coordinating these events remain largely unknown. Here we found that different classes of interneurons use distinct routes of migration to reach the embryonic cerebral cortex. Somatostatin-expressing interneurons that migrate through the marginal zone develop into Martinotti cells, one of the most distinctive classes of cortical interneurons. For these cells, migration through the marginal zone is linked to the development of their characteristic layer 1 axonal arborization. Altering the normal migratory route of Martinotti cells by conditional deletion of Mafb—a gene that is preferentially expressed by these cells—cell-autonomously disrupts axonal development and impairs the function of these cells in vivo. Our results suggest that migration and axon targeting programs are coupled to optimize the assembly of inhibitory circuits in the cerebral cortex
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