Academic Leadership: Gatekeeping or Groundskeeping?

Common approaches to academic leadership include serving as assessors of the progress of individuals towards organizationally determined milestones and markers of success. Likewise, leadership development often focuses on leadership skills and tactics, rather than on cultivation and enactment of leadership philosophies and progressive vision. Here, I discuss the importance of cultivating leadership for progressive faculty and academic staff development through strategically tending the cultures and systems that one leads, in addition to tactical supervision of people. I describe this as systems-engaged leadership manifested as groundskeeping, or as attending to the individuals in an organization while simultaneously actively tending the ecosystems in which the work of the organization occurs. Groundskeeping contrasts with more traditional approaches of leading, which function as gatekeeping, or primarily via guarding who gains access and who advances based on conceptualizations and assumptions about who can function and thrive

Light-dependent governance of cell shape dimensions in cyanobacteria

The regulation of cellular dimension is important for the function and survival of cells. Cellular dimensions, such as size and shape, are regulated throughout the life cycle of bacteria and can be adapted in response to environmental changes to fine-tune cellular fitness. Cell size and shape are generally coordinated with cell growth and division. Cytoskeletal regulation of cell shape and cell wall biosynthesis and/or deposition occurs in a range of organisms. Photosynthetic organisms, such as cyanobacteria, particularly exhibit light-dependent regulation of morphogenes and generation of reactive oxygen species and other signals that can impact cellular dimensions. Environmental signals initiate adjustments of cellular dimensions, which may be vitally important for optimizing resource acquisition and utilization or for coupling the cellular dimensions with the regulation of subcellular organization to maintain optimal metabolism. Although the involvement of cytoskeletal components in the regulation of cell shape is widely accepted, the signaling factors that regulate cytoskeletal and other distinct components involved in cell shape control, particularly in response to changes in external light cues, remain to be fully elucidated. In this review, factors impacting the inter-coordination of growth and division, the relationship between the regulation of cellular dimensions and central carbon metabolism, and consideration of the effects of specific environment signals, primarily light, on cell dimensions in cyanobacteria will be discussed. Current knowledge about the molecular bases of the light-dependent regulation of cellular dimensions and cell shape in cyanobacteria will be highlighted

The Regulation of Light Sensing and Light-Harvesting Impacts the Use of Cyanobacteria as Biotechnology Platforms

Light is harvested in cyanobacteria by chlorophyll-containing photosystems embedded in the thylakoid membranes and phycobilisomes (PBSs), photosystem-associated light-harvesting antennae. Light absorbed by the PBSs and photosystems can be converted to chemical energy through photosynthesis. Photosynthetically-fixed carbon pools, which are constrained by photosynthetic light capture versus the dissipation of excess light absorbed, determine the available organismal energy budget. The molecular bases of the environmental regulation of photosynthesis, photoprotection and photomorphogenesis are still being elucidated in cyanobacteria. Thus, the potential impacts of these phenomena on the efficacy of developing cyanobacteria as robust biotechnological platforms require additional attention. Current advances and persisting needs for developing cyanobacterial production platforms that are related to light sensing and harvesting include the development of tools to balance the utilization of absorbed photons for conversion to chemical energy and biomass versus light dissipation in photoprotective mechanisms. Such tools can be used to direct energy to more effectively support the production of desired bioproducts from sunlight

Phytochrome-dependent coordinate control of distinct aspects of nuclear and plastid gene expression during anterograde signaling and photomorphogenesis

Light perception by photoreceptors impacts plastid transcription, development, and differentiation. This photoreceptor-dependent activity suggests a mechanism for photoregulation of gene expression in the nucleus and plastid that serves to coordinate expression of critical genes of these two organelles. This coordinate expression is required for proper stoichiometric accumulation of components needed for assembly of plastids, photosynthetic light-harvesting complexes and components such as phytochromes. Chloroplast-targeted sigma factors, which function together with the plastid-encoded RNA polymerase to regulate expression of plastid-encoded genes, and nuclear-encoded plastid development factors, such as GLK1 and GLK2, are targets of phytochrome regulation. Such phytochrome-dependent functions are hypothesized to allow light-dependent regulation, and feasibly tuning, of plastid components and function in response to changes in the external environment, which directly affects photosynthesis and the potential for light-induced damage. When the size and protein composition of the light-harvesting complexes are not tuned to the external environment, imbalances in electron transport can impact the cellular redox state and cause cellular damage. We show that phytochromes specifically regulate the expression of multiple factors that function to modulate plastid transcription and, thus, provide a paradigm for coordinate expression of the nuclear and plastid genomes in response to changes in external light conditions. As phytochromes respond to changes in the prevalent wavelengths of light and light intensity, we propose that specific phytochrome-dependent molecular mechanisms are used during light-dependent signaling between the nucleus and chloroplast during photomorphogenesis to coordinate chloroplast development with plant developmental stage and the external environment

Building and Sustaining Diverse Functioning Networks Using Social Media and Digital Platforms to Improve Diversity and Inclusivity

There has long been a focus on building inclusion and diversity in the sciences through a range of efforts intended to increase representation and access. Despite expansive efforts supported by higher education institutions, funding agencies and others, a need persists to support broad participation and success. Digital platforms, including blogs, and social media such as Twitter™, offer emergent paths for scientists to proactively build supportive communities, even where structural diversity or numerical representation of diverse groups remains low. Use of these platforms can range from community building, to proactive mentoring, and advocacy, as well as more customary uses for supporting scholarly success of diverse individuals, including dissemination and accessible discussions of research findings. I discuss specific uses of social-media digital platforms for building and cultivating communities of underrepresented scholars and facilitating engagement around issues of broad concern to groups underrepresented in science and higher education. These uses include mentoring and support to promote equity, inclusion and diversity, promoting self-definition and personal agency, community building, and advocacy. I draw on published literature about using social media and digital platforms in higher education to build and cultivate “social networks” for connecting widely distributed individuals from underrepresented backgrounds to cultivate communities of interest, support and practice, including a focus on mentoring, sponsorship, and advocacy. I highlight the power of Twitter™ and social media platforms to build and cultivate connections of individuals underrepresented in science and the academy and to offer meaningful means for mitigating local deficits related to low structural diversity and inequity

ISSUES IN BIOTECHNOLOGY TEACHING - Teaching the principles of biotechnology transfer: A service-learning approach

As concerns about genetically modified crops, biotechnology and technology transfer have come to the forefront of media coverage and governmental policies, such issues clearly have implications on the life of every citizen around the world. To combat uncertainties about biotechnology and technology transfer with firsthand knowledge of these subjects, a biotechnology service-learning course was designed. This class examines interdisciplinary issues regarding the transfer of biotechnology and agricultural and medical technologies through the utilization of didactic and service-learning activities. The course provides a forum to discuss global issues with respect to biotechnology transfer, which are becoming more commonly addressed by scientists, social scientists and politicians in our society. The objectives of this undergraduate course are (1) to broaden the perspective of students on global issues related to technology transfer from developed countries to developing countries, (2) to examine the efficacy of technology transfer to developing countries through case studies, (3) to critically evaluate current opinions regarding the benefits and costs of technology transfer to both developed and developing countries, and (4) to become aware of and involved with community organizations addressing the needs for and methodologies involved in technology transfer to developing countries

Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells

Fremyella diplosiphon is a freshwater, filamentous cyanobacterium that exhibits light-dependent regulation of photosynthetic pigment accumulation and cellular and filament morphologies in a well-known process known as complementary chromatic adaptation (CCA). One of the techniques used to investigate the molecular bases of distinct aspects of CCA is confocal laser scanning microscopy (CLSM). CLSM capitalizes on the autofluorescent properties of cyanobacterial phycobiliproteins and chlorophyll a. We employed CLSM to perform spectral scanning analyses of F. diplosiphon strains grown under distinct light conditions. We report optimized utilization of CLSM to elucidate the molecular basis of the photoregulation of pigment accumulation and morphological responses in F. diplosiphon

Light Quantity Affects the Regulation of Cell Shape in Fremyella diplosiphon

In some cyanobacteria, the color or prevalent wavelengths of ambient light can impact the protein or pigment composition of the light-harvesting complexes. In some cases, light color or quality impacts cellular morphology. The significance of changes in pigmentation is associated strongly with optimizing light absorption for photosynthesis, whereas the significance of changes in light quality-dependent cellular morphology is less well understood. In natural aquatic environments, light quality and intensity change simultaneously at varying depths of the water column. Thus, we hypothesize that changes in morphology that also have been attributed to differences in the prevalent wavelengths of available light may largely be associated with changes in light intensity. Fremyella diplosiphon shows highly reproducible light-dependent changes in pigmentation and morphology. Under red light (RL), F. diplosiphon cells are blue-green in color, due to the accumulation of high levels of phycocyanin, a RL-absorbing pigment in the light-harvesting complexes or phycobilisomes (PBSs), and the shape of cells are short and rounded. Conversely, under green light (GL), F. diplosiphon cells are red in color due to accumulation of GL-absorbing phycoerythrin in PBSs, and are longer and brick-shaped. GL is enriched at lower depths in the water column, where overall levels of light also are reduced, i.e., to 10% or less of the intensity found at the water surface. We hypothesize that longer cells under low light intensities at increasing depths in the water column, which are generally also enriched in green wavelengths, are associated with greater levels of total photosynthetic pigments in the thylakoid membranes. To test this hypothesis, we grew F. diplosiphon under increasing intensities of GL and observed whether the length of cells diminished due to reduced pressure to maintain larger cells and the associated increased photosynthetic membrane capacity under high light intensity, independent of whether it is light of green wavelengths

From Phenotype to Genotype: Exploring Middle School Students' Understanding of Genetic Inheritance in a Web-Based Environment

Research shows that students face challenges as they learn about genetic inheritance. The challenges could emanate from the fact that genetic inheritance involves unseen processes at different organizational levels. We explored students' understanding of heredity and related concepts such as cells and reproduction using a Web-based Science Inquiry Environment (WISE) curriculum unit that was developed to help middle school students learn about genetic inheritance. Our findings suggest that students made significant gains from pretest to posttest. However, despite overall gains, some students struggled to explain the importance of mitotic and meiotic divisions in transferring genetic information.</jats:p