Hydroxypropyl methylcellulose-beeswax edible coatings formulated with antifungal food additives to reduce alternaria black spot and maintain postharvest quality of cold-stored cherry tomatoes

Edible composite coatings based on hydroxypropyl methylcellulose (HPMC), beeswax (BW), and food preservatives with antifungal properties were formulated and evaluated on cherry tomatoes during cold storage. Selected food preservatives included: sodium methyl paraben (SMP), sodium ethyl paraben (SEP) and sodium benzoate (SB). Cherry tomatoes artificially inoculated with Alternaria alternata were coated and stored up to 21 d at 5 degrees C followed by 4 d of shelf-life at 20 degrees C. All antifungal coatings reduced the incidence and severity of alternaria black spot on inoculated cherry tomatoes, being the SB-based coating the most effective. Analytical and sensory fruit quality was evaluated on intact and cold-stored tomatoes. In contrast to coatings containing SMP or SEP, the SB-based coating was effective to reduce weight loss and respiration rate and maintain the firmness of coated cherry tomatoes. Peel color, ethanol and acetaldehyde content of the juice, sensory flavor, off-flavors, and fruit appearance were not adversely affected by the application of the antifungal coatings. In conclusion, HPMC-BW coatings containing the food additive SB at 2% showed potential for industrial application, including the production and commercialization of organic cherry tomatoes. (C) 2015 Elsevier B.V. All rights reserved

Determination of CO2 solubility in Perna perna mussel and analysis of the suitability of the ideal and non-ideal gas models

In SGS (Soluble Gas Stabilization) processes, the carbon dioxide (CO2 ) is dissolved into the food product under controlled temperature, pressure, and gas/product ratio. The prediction of CO2 solubility can be achieved using a computational code using equations of state from experimental data on CO2 concentration in food. In this work, the solubility of CO2 in Perna perna mussels was obtained using Ideal Gas law and Virial, Van der Waals, Soave-Redlich-Kwong, and Peng-Robinson equations. The SGS process was performed at varying system pressure (200–600 kPa), temperature (0–6 °C), and gas/product (g/p) (mussels) ratio (1:1–5:1) in the cooked and shucked mussels for 65 h. A total of 11 experiments, arranged in a 23 experimental design, with triplicate runs at the central point, were performed. The compressibility factor indicated that the Ideal Gas state is a good approximation only for experiments 1, 2, 5, and 6. It was observed that the pressure and the gas/product ratio exert a more significant influence on the CO2 solubilization process in the mussel. Visual Basic for Applications (VBA) to perform thermodynamic calculations showed to be a great resource regarding complex calculations

Effect of antifungal hydroxypropyl methylcellulose- beeswax edible coatings on gray mold development and quality attributes of cold-stored cherry tomato fruit

Edible composite coatings based on hydroxypropyl methylcellulose (HPMC), beeswax (BW), and food preservatives with antifungal properties, were evaluated on cherry tomatoes during cold storage. Food preservatives selected from previous research work included sodium propionate (SP), potassium carbonate (PC), ammonium phosphate (APh) and ammonium carbonate (AC). Cherry tomatoes artificially inoculated with Botrytis cinerea were coated and stored up to 15 d at 5 degrees C followed by 7 d of shelf-life at 20 degrees C. All antifungal HPMC-BW coatings significantly reduced gray mold development on inoculated and cold-stored cherry tomatoes, the SP-based coating being the most effective. Analytical and sensory fruit quality was also evaluated after cold storage and shelf-life. The AC-based coating was the most effective to control weight loss and maintain the firmness of coated cherry tomatoes. Respiration rate, firmness, color, sensory flavor, off-flavor, and fruit appearance were not adversely affected by the application of the antifungal coatings. Overall, the application of HPMC-BW edible composite coatings containing AC could be a promising treatment to extend the postharvest life of cherry tomatoes. Further studies should focus on the modification of some physical characteristics of the coatings in order to enhance the general performance and provide higher peel gloss. (C) 2014 Elsevier B.V. All rights reserved

Antifungal activity of food additives in vitro and as ingredients of hydroxypropyl methylcellulose-lipid edible coatings against Botrytis cinerea and Alternaria alternata on cherry tomato fruit

The antifungal activity of food additives or 'generally recognized as safe' (GRAS) compounds was tested in vitro against Botrytis cinerea and Alternaria alternate. Radial mycelial growth of each pathogen was measured in PDA Petri dishes amended with food preservatives at 0.2, 1.0, or 2.0% (v/v) after 3, 5, and 7 days of incubation at 25 degrees C. Selected additives and concentrations were tested as antifungal ingredients of hydroxypropyl methylcellulose (HPMC)-lipid edible coatings. The curative activity of stable coatings was tested in in vivo experiments. Cherry tomatoes were artificially inoculated with the pathogens, coated by immersion about 24 h later, and incubated at 20 degrees C and 90% RH. Disease incidence and severity (lesion diameter) were determined after 6, 10, and 15 days of incubation and the 'area under the disease progress stairs' (AUDPS) was calculated. In general, HPMC-lipid antifungal coatings controlled black spot caused by A. alternate more effectively than gray mold caused by B. cinerea. Overall, the best results for reduction of gray mold on cherry tomato fruit were obtained with coatings containing 2.0% of potassium carbonate, ammonium phosphate, potassium bicarbonate, or ammonium carbonate, while 2.0% sodium methylparaben, sodium ethylparaben, and sodium propylparaben were the best ingredients for coatings against black rot. (C) 2013 Elsevier B.V. All rights reserved

Antifungal activity of food additives in vitro and as ingredients of hydroxypropyl methylcellulose-lipid edible coatings against Botrytis cinerea and Alternaria alternata on cherry tomato fruit

The antifungal activity of food additives or 'generally recognized as safe' (GRAS) compounds was tested in vitro against Botrytis cinerea and Alternaria alternate. Radial mycelial growth of each pathogen was measured in PDA Petri dishes amended with food preservatives at 0.2, 1.0, or 2.0% (v/v) after 3, 5, and 7 days of incubation at 25 degrees C. Selected additives and concentrations were tested as antifungal ingredients of hydroxypropyl methylcellulose (HPMC)-lipid edible coatings. The curative activity of stable coatings was tested in in vivo experiments. Cherry tomatoes were artificially inoculated with the pathogens, coated by immersion about 24 h later, and incubated at 20 degrees C and 90% RH. Disease incidence and severity (lesion diameter) were determined after 6, 10, and 15 days of incubation and the 'area under the disease progress stairs' (AUDPS) was calculated. In general, HPMC-lipid antifungal coatings controlled black spot caused by A. alternate more effectively than gray mold caused by B. cinerea. Overall, the best results for reduction of gray mold on cherry tomato fruit were obtained with coatings containing 2.0% of potassium carbonate, ammonium phosphate, potassium bicarbonate, or ammonium carbonate, while 2.0% sodium methylparaben, sodium ethylparaben, and sodium propylparaben were the best ingredients for coatings against black rot. (C) 2013 Elsevier B.V. All rights reserved

COMPARISON OF GINGER (Zingiber officiale Roscoe) OLEORESIN OBTAINED WITH ETHANOL AND ISOPROPANOL WITH THAT OBTAINED WITH PRESSURIZED CO2

Ginger (Zingiber officinale Roscoe) belongs to the Zingiberacea family. It is a spice of great commercial importance. In this work ginger oleoresin was obtained with ethanol, isopropanol and liquid carbon dioxide. The chemical compositions of the extract were compared with each other. All oleoresin samples had monoterpenes and sesquiterpenes. Carboxylic acids were found in organic solvent extracts for an extraction time of 2 hours. The component responsible the for pungent characteristic of the oleoresin, gingerois, were detected in samples obtained with organic solvent for extraction times of 6 hours and in samples obtained with CO2 liquid for extraction times of 2 hours

COMPARISON OF GINGER (Zingiber officiale Roscoe) OLEORESIN OBTAINED WITH ETHANOL AND ISOPROPANOL WITH THAT OBTAINED WITH PRESSURIZED CO2

Ginger (Zingiber officinale Roscoe) belongs to the Zingiberacea family. It is a spice of great commercial importance. In this work ginger oleoresin was obtained with ethanol, isopropanol and liquid carbon dioxide. The chemical compositions of the extract were compared with each other. All oleoresin samples had monoterpenes and sesquiterpenes. Carboxylic acids were found in organic solvent extracts for an extraction time of 2 hours. The component responsible the for pungent characteristic of the oleoresin, gingerois, were detected in samples obtained with organic solvent for extraction times of 6 hours and in samples obtained with CO2 liquid for extraction times of 2 hours.O gengibre (Zingiber officinale Roscoe) pertence à família Zingiberacea. É uma especiaria de grande importância comercial. Neste trabalho realizou-se extrações da oleoresina de gengibre com etanol, isopropanol e dióxido de carbono líquido e comparou-se a composição química de cada um dos extratos. A oleoresina obtida tem componentes das classes dos monoterpenos e sesquiterpenos em todas as amostras. Ácidos carboxílicos foram encontrados nos extratos obtidos com solvente orgânico, quando o tempo de extração foi de 2 horas. Os componentes que dão a característica pungente à oleoresina, os gingerois, foram detectados nas amostras obtidas com solvente orgânico, quando o tempo de extração foi de 6 horas e, nas amostras obtidas com CO2 líquido, com 2 horas de extração.40841