Characterization of 2S albumin allergenic proteins for anaphylaxis in common buckwheat

2S albumin (2SA) is responsible for anaphylaxis following consumption of buckwheat in allergic individuals. To reduce allergen incidents, characterization of 2SA polypeptides is prerequisite, thus was analyzed in this study. Of the five 2S albumin genes (g03, g11, g13, g14, and g28), g03 was seemingly non-functional. The g14 content was 3- and 40-fold higher than that of g11/g28 and g13, respectively. The g11/g28 were more processed to a ∼8 kDa band from a 16 kDa band than g14 in seeds, agreeing with that g11/g28 have high similarity with Fag e 8kD. Meanwhile, anti-g13 produced only a single ∼10 kDa band. Modification of g13 and domain exchange between g13 and g14 suggested that the hydrophobicity of the first domain and the nature of some amino acids in g13 contributed, at least in part, to the lower apparent molecular weight of g13 than expected. Thus, g13 might be an unexplored and noteworthy allergen

2S albumin g13 polypeptide, less related to Fag e 2, can be eliminated in common buckwheat (Fagopyrum esculentum Moench) seeds

2S albumin (g11, g13, g14, and g28) is an important allergen in common buckwheat (Fagopyrum esculentum). g13 is hydrophobic, rare in seeds, and may show distinct allergenicity from the others; therefore, we tried to eliminate this protein. Phylogenetic and property distance analyses indicated g13 is less related to g14 (Fag e 2) than g11/g28 is related to g14, particularly in the second domain containing the II and III α-helices. A null allele with a 531 bp insertion in the coding region was found for g13 at an allele frequency of 2 % in natural populations of common buckwheat. The g13_null allele homozygote accumulated no g13 protein. A BLAST search for the 531 bp insertion suggested the insert-like sequence resided frequently in the buckwheat genome, including the self-incompatibility responsible gene ELF3 in Fagopyrum tataricum. The g13_null insert-like sequence could, therefore, help in producing hypoallergenic cultivars, and expand the genetic diversity of buckwheat

低アレルゲンソバ育成のための種子貯蔵タンパク質の多様性に関する研究

京都大学0048新制・課程博士博士(農学)甲第22504号農博第2408号新制||農||1077(附属図書館)学位論文||R2||N5284(農学部図書室)京都大学大学院農学研究科農学専攻(主査)教授 白岩 立彦, 教授 丸山 伸之, 准教授 田中 朋之学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDGA

Screening of Onion (Allium cepa L.) Genotypes for Acid Tolerance Based on Morpho-physiological and Yield Associated Traits

Onion is one of the most valuable vegetable crops grown all over the world, but its production is severely affected by abiotic stresses like drought, waterlogging, and the acidic nature of the soil. An experiment was conducted to study the morphological and yield contributing characters of four onion genotypes (Indian Onion-1, Indian Onion-2, Indian Onion-3, and Local onion) in the acidic soil condition at Sylhet region, Bangladesh. The experiment was laid out in a randomized complete block design with three replications. Results showed that four genotypes of onion differed significantly for all the morphological and yield characters. The total yield was positively correlated with plant height, bulb fresh weight, bulb diameter, bulb length, leaf sheath fresh weight, leaf sheath dry weight, root fresh and dry weight. Considering yield and yield attributing traits, Indian Onion-2 performed better in acidic soil and had the highest in bulb fresh weight (72.60 g), total yield (1.78 t.ha-1), and moisture content, followed by Indian Onion-3. Therefore, Indian Onion-2 can be selected as the best genotype for acidic soil in the Sylhet region, Bangladesh

Insertion of ten amino acids into 13S globulin zero-repeat subunit improves trypsin digestibility in common buckwheat (Fagopyrum esculentum Moench) seeds

The 13S globulin zero-repeat subunit is resistant to trypsin and may have higher allergenicity than the 1–6 tandem repeat subunits in common buckwheat (Fagopyrum esculentum Moench). To explore alleles useful for lowering allergenicity, amplicon deep sequencing targeting the zero-repeat subunit gene was conducted in bulked genomic DNA from eight cultivars and landraces. The analysis identified a unique allele encoding a zero-repeat subunit with 10 amino acid insertion (10aa) at a position equivalent to the tandem repeat insertion. Prediction of its 3-D structure suggested that 10aa changes the β-hairpin structure in the non-10aa (native) subunit to a random coil, which is also found in 1- and 3- repeat subunits. Homozygotes of the 10aa allele were developed and showed that the 10aa subunit was more digestible than the native subunit. However, the 10aa subunit was still less digestible than the 1–6 repeat subunits, suggesting needs to explore unfunctional alleles

Evaluation of Rapeseed-mustard Genotypes in Different Sowing Regimes and Their Genetic Variabilities

Rapeseed-mustard is an important oilseed species with high nutritional and economic values; it’s popularity increases due to its diverse uses. Ten genotypes of rapeseed-mustard and two sowing dates were evaluated in a split plot design with three replications. Our study demonstrated that sowing dates and genotypes greatly influenced the growth parameters, yield, and it contributing characters. BARI sarisha-16 had the highest yield (1613 kg.ha-1) whereas Binasarisha-9 yielded the lowest yield (840 kg.ha-1). All the yield contributing characters was superior in the earlier (10 November, S1) sowing date than the 24 November (S2), demonstrated by the highest grain yield (1120 kg.ha-1) which was 10 % higher than the seed sown on 24th November (S2) (1025 kg.ha-1), and better higher vegetative growth. These results affirmed that the yield contributing traits resulted the higher seed yield. In most of the studied characters, the higher phenotypic variances were observed than that of its genotypic variances. The significant positive correlation with grain yield were found in plant height, number of primary branches/plants, number of siliqua/plants, siliquae length, number of seed per siliquae, days of 50% flowering to maturity and 1000 seed weight. Therefore, based on the yield and the related traits, BARI sarisha-16 can be used for further utilization

Assessing the genetic diversity of squash (Cucurbita pepo L.) genotypes based on agro-morphological traits and genetic analysis: Evaluation of squash genetic diversity

An experiment was conducted to estimate the genetic variability of 15 indigenous and exotic squash genotypes assessing 18 quantitative and 8 qualitative traits. Results showed that the accessions have high variability in qualitative traits like fruit size, fruit shape, fruit skin colour,lustre and fruit productivity, which allowed selection for considerable gains in these characteristics. The quantitative traits such as fruits yield per plant, fruit weight, length, diameter and total yield per hectare showed the greater phenotypic coefficient of variation (PCV) along with higher heritability which can helps to identify desirable genotypes. The obtained significant and positive correlation between fruit yield with number of leaves, nodes, fruit length, weight and number could assist in selection to improve this crop. Cluster analysis resulted in the formation of 4 groups, confirming the genetic variability among the studied genotypes. Eventually, the attained PCA analysis result revealed that the number of fruits per plant, fruit yield per plant, fruit length and days to first female flowering are the most discriminating traits which are accelerating the variability in squash genotypes. On the basis of the yield and its attributing traits, First Runner is the best genotype suited in this environment