Kid–Kis complexes (molar ratio of 1:1) interact tightly with region I

<p><b>Copyright information:</b></p><p>Taken from "Interactions of Kid–Kis toxin–antitoxin complexes with the operator-promoter region of plasmid R1 are piloted by the Kis antitoxin and tuned by the stoichiometry of Kid–Kis oligomers"</p><p></p><p>Nucleic Acids Research 2007;35(5):1737-1749.</p><p>Published online 21 Feb 2007</p><p>PMCID:PMC1865072.</p><p>© 2007 The Author(s)</p> Macromolecular native mass spectrometry was performed on Kid–Kis and on Kid–Kis– DNA complexes in ammonium acetate (50 mM), pH 5.8. () Mass spectrum of a mixture of Kid:Kis at a molar ratio of 1:1 (Kis 15 μM) and () and () mass spectra of Kid:Kis: DNA mixtures at molar ratios of 40:40:1 and 10:10:1 (Kis 15 μM), respectively. Kid and Kis are indicated with blue rectangles and orange ellipses, respectively, and the DNA fragment with double strand. Each complex is represented by an appropriate combination of rectangles, ellipses and/or DNA double strand. Molecular masses and relative amounts of complexes are shown in Supplementary Tables 1 and 2, respectively

Macromolecular tandem mass spectrometry reveals topology of Kid–Kis– DNA region I complexes

<p><b>Copyright information:</b></p><p>Taken from "Interactions of Kid–Kis toxin–antitoxin complexes with the operator-promoter region of plasmid R1 are piloted by the Kis antitoxin and tuned by the stoichiometry of Kid–Kis oligomers"</p><p></p><p>Nucleic Acids Research 2007;35(5):1737-1749.</p><p>Published online 21 Feb 2007</p><p>PMCID:PMC1865072.</p><p>© 2007 The Author(s)</p> Tandem mass spectrometry was performed on Kid–Kis and Kid–Kis– DNA in ammonium acetate (50 mM), pH 5.8. () Tandem mass spectra of Kis–Kid–Kid–Kis after selection of the 20 ion and () tandem mass spectra of Kis–Kid–Kid–Kis– DNA complex after selection of the 21 ion. Acceleration voltages varied between 25 and 65 V. Kid and Kis are indicated with blue rectangles and orange ellipses, respectively and the DNA fragment with double strand. Each complex is represented by an appropriate combination of rectangles, ellipses and/or DNA double strand