Macromolecular tandem mass spectrometry reveals topology of Kid–Kis– DNA region I complexes

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Interactions of Kid–Kis toxin–antitoxin complexes with the operator-promoter region of plasmid R1 are piloted by the Kis antitoxin and tuned by the stoichiometry of Kid–Kis oligomers"</p><p></p><p>Nucleic Acids Research 2007;35(5):1737-1749.</p><p>Published online 21 Feb 2007</p><p>PMCID:PMC1865072.</p><p>© 2007 The Author(s)</p> Tandem mass spectrometry was performed on Kid–Kis and Kid–Kis– DNA in ammonium acetate (50 mM), pH 5.8. () Tandem mass spectra of Kis–Kid–Kid–Kis after selection of the 20 ion and () tandem mass spectra of Kis–Kid–Kid–Kis– DNA complex after selection of the 21 ion. Acceleration voltages varied between 25 and 65 V. Kid and Kis are indicated with blue rectangles and orange ellipses, respectively and the DNA fragment with double strand. Each complex is represented by an appropriate combination of rectangles, ellipses and/or DNA double strand