IMPROVING ICE CREAM DELIVERIES TO RETAIL AND INSTITUTIONAL OUTLETS

The author outlines the problems encountered in the present ice cream delivery systems to retail outlets and makes suggestions for improvements.Agribusiness,

Privileged burial in the Pava Pieve (Siena, 8th century AD)

During the 6th archaeological excavation campaign performed at the «Pieve di Pava» (San Giovanni d’Asso, Siena, Italy) in the summer of 2009, a stone-lined burial of a high-status single individual (US 2378) was discovered, covered by a monolithic slab and placed in front of the altar. The tomb is about 160 cm long, 40 cm wide and over 70 cm deep. The skeletal remains of a young male (18-20 years), not in anatomical connection, were found on the floor of the tomb. 14C dating revealed a period between 650 and 688 AD. Stable isotope analysis (18O, 13C, 15N) attested that he was a member of the local community, with a diet quite rich in animal proteins. We are in presence of the secondary burial of an eminent personage, perhaps a saint, likely to have been transported to the church in a sack of perishable material, possibly textile, which caused their alignment along a curved line, as clearly demonstrated by the circular delimitation of the bones. The body was probably used for the re-consecration of the church, following the restoration works of the 8th century. The paleopathological study diagnosed a case of acromesomelic dysplasia, a congenital anomaly with disproportionate limbs: short, enlarged distal segments (radius-ulna and tibia), almost normal proximal segments (humerus and femur), short stature of about 150 cm, and bilateral fibular agenesis. Tibiae malformation and fibulae agenesis led to bilateral talipes valgus, with major walking problems. Extensive enthesopathies in the upper limbs indicate the use of crutches

Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers

<p>Abstract</p> <p>Background</p> <p>Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers might unveil the molecular basis of viral transmission. Quantitative real-time RT-PCR (RT-qPCR) is currently the most accurate and sensitive method used for quantitative gene expression analysis. In order to normalize the resulting quantitative data, reference genes with constant expression during the experimental procedures are needed.</p> <p>Results</p> <p>Partial sequences of the commonly used reference genes actin (ACT), α1-tubulin (TUB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1A), ribosomal protein S18 (RPS18) and polyubiquitin C (UBI) from <it>Delphacodes kuscheli</it>, a planthopper capable of persistently transmitting the plant fijivirus <it>Mal de Río Cuarto virus </it>(MRCV), were isolated for the first time. Specific RT-qPCR primers were designed and the expression stability of these genes was assayed in MRCV-infective and naïve planthoppers using geNorm, Normfinder and BestKeeper tools. The overall analysis showed that UBI, followed by 18S and ACT, are the most suitable genes as internal controls for quantitative gene expression studies in MRCV-infective planthoppers, while TUB and EF1A are the most variable ones. Moreover, EF1A was upregulated by MRCV infection.</p> <p>Conclusions</p> <p>A RT-qPCR platform for gene expression analysis in the MRCV-infected planthopper vector <it>Delphacodes kuscheli </it>was developed. Our work is the first report on reference gene selection in virus-infected insects, and might serve as a precedent for future gene expression studies on MRCV and other virus-planthopper pathosystems.</p

Antioxidant activity in Pothomorphe peltata

Abstract Leaves of Pothomorphe peltata (L.) Miq. (Piperaceae) are used locally as anti-inflammatory, antipyretic, hepatoprotective and diuretic infusions and to treat external ulcers and local infections in several parts of the Peruvian, Bolivian and Brazilian Amazon region. The antioxidant activity of different extracts of P. peltata was studied using the hydroperoxide-initiated chemiluminescence assay in liver homogenates, and the methanolic extract was found to have the highest antioxidant activity, with an IC 50 = 4 µg/ml. Aqueous and dichloromethane extracts did not show antioxidant activity. The extracts were further evaluated using the thiobarbituric acid-reactive substances (TBARS) assay. Finally, an assay of DNA sugar damage induced by Fe (II) salt was used to determine the capacity of the extracts to suppress the oxidative degradation of DNA. All the extracts showed antioxidant activity in the latter two bioassays. The methanolic extract showed the highest activity in reducing oxidative damage to DNA, with an IC 50 = 5 µg/ml. Since this extract was highly effective in reducing chemiluminescence and DNA damage, and because the latter activity could be due to the presence of compounds that bind to DNA, DNA-binding activity was studied using the DNAmethyl green (DNA-MG) bioassay. A 30% decrease in the initial absorbance of DNA-MG complex was observed in the methanolic extract at 1000 µg/ml, suggesting the presence of compounds that bind to genetic material. No DNA-binding activity was observed in the aqueous or dichloromethane extracts

DNA methylase modifications and other linezolid resistance mutations in coagulase-negative staphylococci in Italy

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Precision measurement of the E2E2 transition strength to the 21+^+_1 state of 12^{12}C

The form factor of the electromagnetic excitation of $^{12}$C to its 2$^+_1$ state was measured at extremely low momentum transfers in an electron scattering experiment at the S-DALINAC. A combined analysis with the world form factor data results in a reduced transition strength $B(E2; 2^+_1\rightarrow 0^+_1) =7.63(19)$ e$^2$fm$^4$ with an accuracy improved to 2.5\%. In-Medium-No Core Shell Model results with interactions derived from chiral effective field theory are capable to reproduce the result. A quadrupole moment $Q(2^+_1) = 5.97(30)$ efm$^2$ can be extracted from the strict correlation with the $B((E2)$ strength emerging in the calculations.Comment: 6 pages, 6 figures, submitted to Phys. Rev.

A Monitoring Framework with Integrated Sensing Technologies for Enhanced Food Safety and Traceability

A novel and low-cost framework for food traceability, composed by commercial and proprietary sensing devices, for the remote monitoring of air, water, soil parameters and herbicide contamination during the farming process, has been developed and verified in real crop environments. It offers an integrated approach to food traceability with embedded systems supervision, approaching the problem to testify the quality of the food product. Moreover, it fills the gap of missing low-cost systems for monitoring cropping environments and pesticides contamination, satisfying the wide interest of regulatory agencies and final customers for a sustainable farming. The novelty of the proposed monitoring framework lies in the realization and the adoption of a fully automated prototype for in situ glyphosate detection. This device consists of a custom-made and automated fluidic system which, leveraging on the Molecularly Imprinted Polymer (MIP) sensing technology, permits to detect unwanted glyphosate contamination. The custom electronic mainboard, called ElectroSense, exhibits both the potentiostatic read-out of the sensor and the fluidic control to accomplish continuous unattended measurements. The complementary monitored parameters from commercial sensing devices are: temperature, relative humidity, atmospheric pressure, volumetric water content, electrical conductivity of the soil, pH of the irrigation water, total Volatile Organic Compounds (VOCs) and equivalent CO (Formula presented.). The framework has been validated during the olive farming activity in an Italian company, proving its efficacy for food traceability. Finally, the system has been adopted in a different crop field where pesticides treatments are practiced. This has been done in order to prove its capability to perform first level detection of pesticide treatments. Good correlation results between chemical sensors signals and pesticides treatments are highlighted

Irisin, the novel myokine responsible for benefits of physical exercise on bone

It has been recently reported that, after physical exercise activity, the skeletal muscle releases Irisin, the newly identified myokine able of driving transition of white adipocytes into brown, following a phenomenon known as the browning response. This result suggested that skeletal muscle is crucial in the regulation of energy homeostasis, supporting its role as endocrine organ that targets adipose tissue by promoting energy expenditure. In accordance with this new finding, we demonstrated that conditioned media (CM) collected from primary myoblasts of exercised mice were able to induce osteoblast differentiation in a greater extent than those of mice housed in resting conditions and this effect is Irisin-mediated. In view of further proving the involvement of Irisin in bone metabolism, we validate its direct effect on osteoblasts by using r-Irisin. Here we show that phosphorylation of MAP kinase ERK and expression of Atf 4 (p&lt;0,001), the key trascription factor of osteoblast differentiation, were significantly increased after Irisin treatment. Furthermore, ALP and pro-Collagen I mRNA resulted up regulated (p&lt;0,001), as we already demonstrated by treating osteoblasts with conditioned medium from primary myoblasts of exercised mice. To recapitulate in vivo the effect of physical exercise, we injected mice with r-Irisin. Our results show that BV/TV of Irisin-treated mice was higher than vehicle-injected mice. In elderly, the severe decline of skeletal muscle function, known as Sarcopenia, is associated with impaired function of bone (Osteopenia) and these two simultaneous losses of function lead to increased risk of bone fractures. In order to reveal new strategies for treatment of sarcopenia and osteopenia, we also analyzed the effect of physical activity in old mice. Our findings demonstrate that mRNA levels of the most relevant bone proteins resulted up regulated in ex-vivo osteoblast obtained from exercised old mice compared with mice kept in resting conditions. Our data highlight a novel link in muscle-fat-bone axis demonstrating that Irisin targets bone tissue directly. Future perspectives, based on these studies, could satisfy the ongoing research of exercise-mimetic therapies with anabolic action on the skeleton