Use of Clinical Mental Health Counseling Student and Client Recording in Practicum: An Overview of CACREP Training Programs in the SACES Region

SACES CACREP accredited clinical mental health counseling practicum training handbooks were explored to discover the designated use of video recording and review during practicum. Results from an artifact review indicated a lack of standardization across programs. Implications for future research and clinical counseling programs practicum experiences are presented

Sedimentological and stratigraphic framework of the several hundred thousand years old lacustrine record from Lake Van, Turkey

Within the frame of the International Continental scientific Drilling Program (ICDP) project PALEOVAN, a long and continuous sediment record from Lake Van, a closed lake situated in a climatically sensitive semiarid and tectonically active region in Eastern Anatolia, has been drilled in summer 2010. At two sites, Ahlat Ridge and Northern Basin, sedimentary records of 220 and 140 m were recovered, respectively. With basal ages possibly around 500'000 years, these records span several glacial-interglacial cycles and reach back until the lake’s initial transgression in the Middle Pleistocene. First results from ongoing analysis of core-catcher samples and newly opened cores document the sedimentological and geochemical succession. Two composite profiles of the drill sites were defined. Core catcher-based geochemical data such as proxies of lake’s productivity and catchment alterations show large variations and reflect a rich paleoenvironmental history. Most of the 220 m thick succession consists of carbonate mud, mostly sub-mm-thick laminated and interbedded by either homogenous mud or pyroclastic cm-thick layers. The lowermost sediments from the Ahlat Ridge site represent the initial lake transition as the drilling could not penetrate further and the seismic data indicates coincidence with the ‘acoustic’ basement. Such an early transgressive state of the lake’s history is also supported by the lithology consisting of a gravel unit as an indicator of a beach-like environment, which is overlain by sand deposits containing fresh-water gastropods (Bithynia). Above 200 mblf, the laminated mud clearly indicates that the lake was already deep enough to form anoxic bottom water as the laminations were preserved. This unique paleoclimate archive indicates that great changes of the depositional conditions occurred that hint to a fascinating evolution of the environment and has ideal prerequisites for the investigation of the Quaternary climate evolution in the Near East

Two critical positions in zinc finger domains are heavily mutated in three human cancer types

A major goal of cancer genomics is to identify somatic mutations that play a role in tumor initiation or progression. Somatic mutations within transcription factors are of particular interest, as gene expression dysregulation is widespread in cancers. The substantial gene expression variation evident across tumors suggests that numerous regulatory factors are likely to be involved and that somatic mutations within them may not occur at high frequencies across patient cohorts, thereby complicating efforts to uncover which ones are cancerrelevant. Here we analyze somatic mutations within the largest family of human transcription factors, namely those that bind DNA via Cys2His2 zinc finger domains. Specifically, to hone in on important mutations within these genes, we aggregated somatic mutations across all of them by their positions within Cys2His2 zinc finger domains. Remarkably, we found that for three classes of cancers profiled by The Cancer Genome Atlas (TCGA)ÐUterine Corpus Endometrial Carcinoma, Colon and Rectal Adenocarcinomas, and Skin Cutaneous Melanoma Ðtwo specific, functionally important positions within zinc finger domains are mutated significantly more often than expected by chance, with alterations in 18%, 10% and 43% of tumors, respectively. Numerous zinc finger genes are affected, with those containing KruÈppel- associated box (KRAB) repressor domains preferentially targeted by these mutations. Further, the genes with these mutations also have high overall missense mutation rates, are expressed at levels comparable to those of known cancer genes, and together have biological process annotations that are consistent with roles in cancers. Altogether, we introduce evidence broadly implicating mutations within a diverse set of zinc finger proteins as relevant for cancer, and propose that they contribute to the widespread transcriptional dysregulation observed in cancer cells

Increased receptor affinity of SARS-CoV-2: a new immune escape mechanism.

‘Affinity escape’: Novel SARS-CoV-2 variants may escape immunity by raising the RBD-ACE2 affinity high enough to outcompete the avidity of neutralizing antibodies

Leveraging biotechnologies to understand the mechanisms by which California market squid achieve camouflage

The California market squid has specialized skin that is able to manipulate light through reversible condensation and assembly of reflectin proteins, allowing the squid to tune its skin iridescence for precise camouflage and underwater communication. Differential phosphorylation of reflectin proteins is observed to be contaminant with the reversible condensation and hierarchical assembly of reflectin proteins. Our mechanistic understanding of the role of protein phosphorylation in reflectin-mediated iridescence remains incomplete due to the inability to obtain large quantities (\u3elmg) of a homogenous population of phosphorylated reflectins. To overcome this limitation, we propose to leverage state-of-the-art biotechnologies. These include 1) an engineered f. coli strain with an expanded genetic code and 2) an orthogonal translation system capable of site-specific incorporation of phosphoserine into proteins. We will aim to mimic the squid by incorporating phosphoserine into reflectin in a genetically encoded manner. To accomplish this, the learning-objectives-by-doing in this interdisciplinary project will be: cloning the reflectin Al gene, constructing mutants, protein expression in a bacterial system, and purification of site-specifically phosphorylated variants of reflectins. Our work will provide the foundation for elucidating the role of phosphorylation in reflectin-mediated camouflage in marine organisms

Teachers’ Perceptions of Good and Bad Leaders in Seven Cultures

Educational practices are becoming increasingly globalized, and as a result, countries around the world are implementing reforms along the lines of American and European practices, although the cultural implications of these changes are not always being fully realized. Only recently have educational thinkers begun to address cultural issues in educational leadership