65 research outputs found
Resolved Measurements of X_(CO) in NGC 6946
We present the largest sample to date of giant molecular clouds (GMCs) in a substantial spiral galaxy other than the Milky Way. We map the distribution of molecular gas with high resolution and image fidelity within the central 5 kpc of the spiral galaxy NGC 6946 in the ^(12)CO (J = 1-0) transition. By combining observations from the Nobeyama Radio Observatory 45 m single dish telescope and the Combined Array for Research in Millimeter Astronomy interferometer, we are able to obtain high image fidelity and accurate measurements of L_CO compared with previous purely interferometric studies. We resolve individual GMCs, measure their luminosities and virial masses, and derive X CO—the conversion factor from CO measurements to H2 masses—within individual clouds. On average, we find that X_CO = 1.2 × 10^(20) cm^(–2) (K km s^(–1))^(–1), which is consistent within our uncertainties with previously derived Galactic values as well as the value we derive for Galactic GMCs above our mass sensitivity limit. The properties of our GMCs are largely consistent with the trends observed for molecular clouds detected in the Milky Way disk, with the exception of six clouds detected within ~400 pc of the center of NGC 6946, which exhibit larger velocity dispersions for a given size and luminosity, as has also been observed at the Galactic center
High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion
Targeted mutagenesis using CRISPR/Cas9 technology has been shown to be a powerful approach to examine gene function in diverse metazoan species. One common drawback is that mixed genotypes, and thus variable phenotypes, arise in the F0 generation because incorrect DNA repair produces different mutations amongst cells of the developing embryo. We report here an effective method for gene knockout (KO) in the hydrozoan Clytia hemisphaerica, by injection into the egg of Cas9/sgRNA ribonucleoprotein complex (RNP). Expected phenotypes were observed in the F0 generation when targeting endogenous GFP genes, which abolished fluorescence in embryos, or CheRfx123 (that codes for a conserved master transcriptional regulator for ciliogenesis) which caused sperm motility defects. When high concentrations of Cas9 RNP were used, the mutations in target genes at F0 polyp or jellyfish stages were not random but consisted predominantly of one or two specific deletions between pairs of short microhomologies flanking the cleavage site. Such microhomology-mediated (MM) deletion is most likely caused by microhomology-mediated end-joining (MMEJ), which may be favoured in early stage embryos. This finding makes it very easy to isolate uniform, largely non-mosaic mutants with predictable genotypes in the F0 generation in Clytia, allowing rapid and reliable phenotype assessment
Reactions of 6-metheoxy-3-azabicyclo[4.2.0]octan-2-one and its 7-substituted derivatives
金沢大学大学院自然科学研究科生理活性物質科学金沢大学薬学
Star formation efficiency in the Barred Spiral Galaxy NGC 4303
We present new CO(J=1-0) observations of the barred galaxy NGC 4303
using the Nobeyama 45m telescope (NRO45) and the Combined Array for Research in
Millimeter-wave Astronomy (CARMA). The H images of barred spiral
galaxies often show active star formation in spiral arms, but less so in bars.
We quantify the difference by measuring star formation rate and efficiency at a
scale where local star formation is spatially resolved. Our CO map covers the
central 2\farcm3 region of the galaxy; the combination of NRO45 and CARMA
provides a high fidelity image, enabling accurate measurements of molecular gas
surface density. We find that star formation rate and efficiency are twice as
high in the spiral arms as in the bar. We discuss this difference in the
context of the Kennicutt-Schimidt (KS) law, which indicates a constant star
formation rate at a given gas surface density. The KS law breaks down at our
native resolution ( 250 pc), and substantial smoothing (to 500 pc) is
necessary to reproduce the KS law, although with greater scatter.Comment: 17 pages, 10 figures, published by ApJ;
http://adsabs.harvard.edu/abs/2010ApJ...721..383
Resolved Giant Molecular Clouds in Nearby Spiral Galaxies: Insights from the CANON CO (1-0) Survey
We resolve 182 individual giant molecular clouds (GMCs) larger than 2.5 × 10^5 M ☉ in the inner disks of 5 large nearby spiral galaxies (NGC 2403, NGC 3031, NGC 4736, NGC 4826, and NGC 6946) to create the largest such sample of extragalactic GMCs within galaxies analogous to the Milky Way. Using a conservatively chosen sample of GMCs most likely to adhere to the virial assumption, we measure cloud sizes, velocity dispersions, and ^(12)CO (J = 1-0) luminosities and calculate cloud virial masses. The average conversion factor from CO flux to H_2 mass (or X_(CO)) for each galaxy is 1-2 × 10^(20) cm^(–2) (K km s^(–1))^(–1), all within a factor of two of the Milky Way disk value (~2 × 10^(20) cm^(–2) (K km s^(–1))^(–1)). We find GMCs to be generally consistent within our errors between the galaxies and with Milky Way disk GMCs; the intrinsic scatter between clouds is of order a factor of two. Consistent with previous studies in the Local Group, we find a linear relationship between cloud virial mass and CO luminosity, supporting the assumption that the clouds in this GMC sample are gravitationally bound. We do not detect a significant population of GMCs with elevated velocity dispersions for their sizes, as has been detected in the Galactic center. Though the range of metallicities probed in this study is narrow, the average conversion factors of these galaxies will serve to anchor the high metallicity end of metallicity-X_(CO) trends measured using conversion factors in resolved clouds; this has been previously possible primarily with Milky Way measurements
Star Formation on Subkiloparsec Scale Triggered by Non-linear Processes in Nearby Spiral Galaxies
We report a super-linear correlation for the star formation law based on new CO(J = 1-0) data from the CARMA and NOBEYAMA Nearby-galaxies (CANON) CO survey. The sample includes 10 nearby spiral galaxies, in which structures at sub-kpc scales are spatially resolved. Combined with the star formation rate surface density traced by Hα and 24 μm images, CO(J = 1-0) data provide a super-linear slope of N = 1.3. The slope becomes even steeper (N = 1.8) when the diffuse stellar and dust background emission is subtracted from the Hα and 24 μm images. In contrast to the recent results with CO(J = 2-1) that found a constant star formation efficiency (SFE) in many spiral galaxies, these results suggest that the SFE is not independent of environment, but increases with molecular gas surface density. We suggest that the excitation of CO(J = 2-1) is likely enhanced in the regions with higher star formation and does not linearly trace the molecular gas mass. In addition, the diffuse emission contaminates the SFE measurement most in regions where the star formation rate is law. These two effects can flatten the power-law correlation and produce the apparent linear slope. The super-linear slope from the CO(J = 1-0) analysis indicates that star formation is enhanced by non-linear processes in regions of high gas density, e.g., gravitational collapse and cloud-cloud collisions
Physical Conditions in Molecular Clouds in the Arm and Interarm Regions of M51
We report systematic variations in the emission line ratio of the CO J = 2-1 and J = 1-0 transitions (R_(2-1/1-0)) in the grand-design spiral galaxy M51. The R_(2-1/1-0) ratio shows clear evidence for the evolution of molecular gas from the upstream interarm regions into the spiral arms and back into the downstream interarm regions. In the interarm regions, R_(2-1/1-0) is typically 0.7 (often 0.8-1.0) in the spiral arms, particularly at the leading (downstream) edge of the molecular arms. These trends are similar to those seen in Galactic GMCs with OB star formation (presumably in the Galactic spiral arms). R_(2-1/1-0) is also high, ~0.8-1.0, in the central region of M51. Analysis of the molecular excitation using a Large Velocity Gradient radiative transfer calculation provides insight into the changes in the physical conditions of molecular gas between the arm and interarm regions: cold and low-density gas (≾ 10 K, ≾ 300 cm^(–3)) is required for the interarm GMCs, but this gas must become warmer and/or denser in the more active star-forming spiral arms. The ratio R_(2-1/1-0) is higher in areas of high 24 μm dust surface brightness (which is an approximate tracer of star formation rate surface density) and high CO(1-0) integrated intensity (i.e., a well-calibrated tracer of total molecular gas surface density). The systematic enhancement of the CO(2-1) line relative to CO(1-0) in luminous star-forming regions suggests that some caution is needed when using CO(2-1) as a tracer of bulk molecular gas mass, especially when galactic structures are resolved
An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica
The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different feeding regimes using Artemia larvae as food and demonstrate that the stage-dependent feeding control is the key for rapid and reliable medusa and polyp rearing. Metamorphosis of the planula larvae into a polyp colony can be induced efficiently using a new synthetic peptide. The optimized procedures detailed here make it practical to generate genetically modified Clytia strains and to maintain their whole life cycle in the laboratory
Two Oppositely Localised Frizzled RNAs as Axis Determinants in a Cnidarian Embryo
In phylogenetically diverse animals, including the basally diverging cnidarians, “determinants” localised within the egg are responsible for directing development of the embryonic body plan. Many such determinants are known to regulate the Wnt signalling pathway, leading to regionalised stabilisation of the transcriptional coregulator β-catenin; however, the only strong molecular candidate for a Wnt-activating determinant identified to date is the ligand Wnt11 in Xenopus. We have identified embryonic “oral–aboral” axis determinants in the cnidarian Clytia hemisphaerica in the form of RNAs encoding two Frizzled family Wnt receptors, localised at opposite poles of the egg. Morpholino-mediated inhibition of translation showed that CheFz1, localised at the animal pole, activates the canonical Wnt pathway, promotes oral fates including gastrulation, and may also mediate global polarity in the ectoderm. CheFz3, whose RNA is localised at the egg vegetal cortex, was found to oppose CheFz1 function and to define an aboral territory. Active downregulation mechanisms maintained the reciprocal localisation domains of the two RNAs during early development. Importantly, ectopic expression of either CheFz1 or CheFz3 was able to redirect axis development. These findings identify Frizzled RNAs as axis determinants in Clytia, and have implications for the evolution of embryonic patterning mechanisms, notably that diverse Wnt pathway regulators have been adopted to initiate asymmetric Wnt pathway activation
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