Multiple, Independent T Cell Lymphomas Arising in an Experimentally FIV-Infected Cat during the Terminal Stage of Infection.

Our laboratory has serially reported on the virologic and immunopathologic features of a cohort of experimental feline immunodeficiency virus (FIV)-infected cats for more than eight years. At 8.09 years post infection (PI), one of these animals entered the terminal stage of infection, characterized by undulating hyperthermia, progressive anorexia, weight loss, and pancytopenia; the animal was not responsive to therapeutic interventions, necessitating euthanasia six weeks later (8.20 years PI). Subsequent analyses indicated that neoplastic lymphocytes infiltrated multiple cervical lymph nodes and a band-like region of the mucosal lamina propria within a segment of the intestine. Immunohistochemistry and T cell clonality testing determined that the nodal and intestinal lesions were independently arising from CD3 T cell lymphomas. In-situ RNA hybridization studies indicated that diffuse neoplastic lymphocytes from the cervical lymph node contained abundant viral nucleic acid, while viral nucleic acid was not detectable in lymphocytes from the intestinal lymphoma lesion. The proviral long terminal repeat (LTR) was amplified and sequenced from multiple anatomic sites, and a common clone containing a single nucleotide polymorphism was determined to be defective in response to phorbol myristate acetate (PMA)-mediated promoter activation in a reporter gene assay. This assay revealed a previously unidentified PMA response element within the FIV U3 region 3' to the TATA box. The possible implications of these results on FIV-lymphoma pathogenesis are discussed

Assessing Barriers to Health Care Access for New Americans

Introduction. Healthcare within the United States has been at the forefront of public discussion and political representation in recent years, particularly as it relates to healthcare access and barriers to said access. Focus has been placed on low-income groups that most generally represent the face of the average American, but this leaves the question: How are new Americans faring, and do their struggles match those faced by the rest of the country? The new Americans of Burlington, Vermont serve as a small window into a unique refugee population’s experiences with healthcare in the United States. Methods. A focus group consisting of 8 women was hosted on-site at the Burlington Housing Authority Franklin Square apartments. Questions were designed to determine demographic data as well facilitate subjective discussion on participants’ healthcare experiences. Translation services were provided by the resident manager. Results. Languages spoken were Mai Mai, Swahili, and English. Five major themes for healthcare access barriers were identified: language barriers, having children, transportation barriers, financial barriers, and a lack of preventative care. Sub- themes were also identified, which formed a taxonomy of barriers to healthcare access among the representative population. Discussion/Conclusions. The difficulties faced by new Americans are numerous and interrelated, leading to a perpetual cycle of insufficient healthcare. Throughout the discussion, the financial burden of healthcare was regularly raised as one of the most prominent issues faced. This concern matches with those found in similar, previous studies, that have analyzed the difficulties faced by the rest of America.https://scholarworks.uvm.edu/comphp_gallery/1254/thumbnail.jp

CHARACTERISING LONGITUDINAL ALTERATIONS IN POSTURAL CONTROL FOLLOWING LOWER LIMB INJURY IN PROFESSIONAL RUGBY UNION PLAYERS

Assessment of player’s postural control following a lower limb injury is of interest to sports science and medicine practitioners due to its fundamental role in daily tasks and sporting activities. The purpose of this study was to measure the longitudinal changes in rugby union player’s postural control throughout return to play (RTP) following lower limb injury. Rehabilitation was divided into three phases – acute, middle and late. Nine players from a professional rugby union team (height 1.80±0.06 m; mass 96.1±13.2 kg; age 25±3 years) were included in this study. Static unilateral postural control was measured in the acute phase using a PASCO dual axis force platform (PS-2142). Dynamic postural control was measured using single axis PASCO force platforms (PS-2141) with the middle phase being assessed by unilateral drop jump and the late phase a unilateral lateral hurdle hop. During the acute phase, no improvement were observed between the initial testing session and end testing session, nor any differences between the end of the acute rehabilitation phase and pre-injury baseline. Whereas for the middle and late phase improvements were observed between the initial and end testing session, with smaller magnitudes of dynamic postural control adjustment (DPCA; p (

PERFORMANCE ASSESSMENTS OF THE MIDDLE AND LATE PHASE OF REHABILITATION FOLLOWING LOWER LIMB INJURY IN PROFESSIONAL RUGBY UNION PLAYERS

The purpose of this study was to characterise the kinetic profile of the jumping strategy employed in rugby union players during the middle and late phases of rehabilitation following lower limb injury. Nine players from a professional rugby union team (height 1.80±0.06 m; mass 96.1±13.2 kg; age 25±3 years) were included in this study. The mean duration of the middle and late phases of rehabilitation were 10±5 weeks and 6±2 weeks respectively. Unilateral drop jump and unilateral lateral hurdle hop were used to characterise the middle and late phases respectively. The variables of interest were Initial peak landing force, ground contact time, net impulse, Instantaneous loading rate, flight time and second peak landing force. Differences were observed in kinetic jump profiles between uniplanar and multiplanar movements. A change in kinetic jumping strategy to attain the same performance magnitudes across both phases of rehabilitation was also observed. The results highlight the importance of practitioners using a range of functional assessments in return to play testing

The Obesity Epidemic

The incidence of chronic, noncommunicable diseases, such as heart disease, is increasing at an alarming rate on the global scale. The growing prevalence of overweight and obesity have led to an upsurge in cases of diabetes and other obesity-related diseases. About 18 million people die every year from heart disease, of which diabetes and obesity are major predisposing factors. Worldwide, more than 1.1 billion adults are overweight, 312 million of which are obese. The number of children that are overweight or obese is also growing (Hossain, Parvez et al.). Obesity, and the associated diseases, has become a worldwide epidemic and must be dealt with before the societal, economic, and security implications become too much to handle

Microsatellites to enhance characterization, conservation and breeding value of Capsicum germplasm

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Diet quality and history of gestational diabetes mellitus among childbearing women, United States, 2007-2010

INTRODUCTION: Women with a history of gestational diabetes mellitus (GDM) have elevated risk of developing type 2 diabetes. Diet quality plays an important role in the prevention of type 2 diabetes. We compared diet quality among childbearing women with a history of GDM with the diet quality of childbearing women without a history of GDM. METHODS: We used data from the National Health and Nutrition Examination Survey for 2007 through 2010. We included women without diabetes aged 20 to 44 years whose most recent live infant was born within the previous 10 years and who completed two 24-hour dietary recalls. The Healthy Eating Index (HEI)-2010 estimated overall and component diet quality. Multivariable linear regression models estimated the association between a history of GDM and current diet quality, adjusting for age, education, smoking status, and health risk for diabetes. RESULTS: A history of GDM was reported by 7.7% of women. Compared with women without a history of GDM, women with a history of GDM had, on average, 3.4 points lower overall diet quality (95% confidence interval [CI], -6.6 to -0.2) and 0.9 points lower score for consumption of green vegetables and beans (95% CI, -1.4 to -0.4). Other dietary component scores did not differ by history of GDM. CONCLUSION: In the United States, women with a history of GDM have lower diet quality compared with women who bore a child and do not have a history of GDM. Improving diet quality may be a strategy for preventing type 2 diabetes among childbearing women

Association between First Trimester Pregnancy Associated Plasma Protein–A and the Development of Gestational Diabetes Mellitus

Background: Gestational diabetes (GDM) is a common pregnancy complication with significant cardiometabolic consequences for mothers and offspring. Previous research from our group suggests that adipose tissue IGFBP-5 and its unique metalloprotease PAPP-A (Pregnancy Associated Plasma Protein-A) may play mechanistic roles in GDM development by regulating functional IGF-1 levels and lipid storage and metabolism. Aim: To examine the relationship between circulating PAPP-A levels and GDM development. We hypothesized that high first trimester PAPP-A levels would be associated with decreased GDM risk. Methods: A retrospective cohort of women delivering singleton gestations at UMass Memorial Healthcare (2009, 2010, 2014, 2015) was assembled by abstracting electronic medical records. PAPP-A was measured in first trimester (11-14 weeks), and reported as quartiles of multiples of the mean (MoM) based on gestational age and adjusted for maternal weight and race/ethnicity. GDM diagnosis based on standard 2-step protocol (~24-28 weeks; failed 50g 1hr glucola screen then ≥2 abnormal values per Carpenter-Coustan criteria on 100g 3hr glucose tolerance test). Crude and multivariable-adjusted logistic regression models estimated the association between PAPP-A MoM quartiles and GDM. Results: Women (N=1,251) were 29.7 (SD:5.7) years old and 12.5 (SD:0.6) weeks gestation at PAPP-A measurement. 7.6% (n=95) developed GDM. Median PAPP-A MoM were 0.7 (inter-quartile range [IQR]=0.5-1.0) among women with GDM and 0.9 (IQR=0.6-1.3) among controls; 39% versus 23% were in the 1st quartile, respectively. After adjusting for pre-pregnancy body mass index, nuchal translucency, crown rump length, smoking status, and parity, women with PAPP-A MoM in 2nd, 3rd, and 4th quartiles had 52% (OR=0.48, 95%CI=0.26-0.88), 45% (OR=0.55, 95%CI=0.30-0.99) and 73% (OR=0.27, 95%CI=0.13-0.53) lower odds of GDM compared to women in the 1st quartile. Conclusion: Higher PAPP-A MoM levels were associated with lower GDM risk. Future studies will assess whether higher PAPP-A levels are associated with enhanced IGF-1 signaling and improved pregnancy metabolic homeostasis