Two Unordered Queues

A special customer must complete service from two servers in series, in either order, each with an M/M/1 queueing system. It is assumed that the two queueing system lengths are independent with initial numbers of customers a and b at the instant when the special customer arrives. We find the expected total time (ETT) for the special customer to complete service. We show that even if the interarrival and service time parameters of two queues are identical, there exist examples (specific values of the parameters and initial lengths) for which the special customer surprisingly has a lower expected total time to completion by joining the longer queue first rather than the shorter one.Comment: Presented at AMMCS 2011 Conference, July 25, 201

Driven: Cultural Hegemony and Reasons for Driving in the United States

United States transportation rates have revealed a driving decline over the last decade. Media sources report that young Americans are less interested in obtaining driver’s licenses than previous generations, citing technology, social media, and graduated driver’s licensing procedures as reasons. Explanations such as expense, lack of time, and the other transportation options, shown in results of survey research, are neglected in media accounts. Interviews I conducted with white suburban drivers reveal that Millennials obtain their licenses to fulfill responsibilities, whereas Generation X, Baby Boomer, and Silent Generation interviewees express that driving was motivated by social expectation, freedom, and independence. I contend that for the second half of the twentieth century, driving became hegemonic, and in the twenty-first century, driving is becoming ideological. The effort of media sources to trivialize Millennials’ motives reflects the broken hegemony of driving, and the ability to question driving in our culture presents potential for change

Enhanced expression of Harvey ras induced by serum deprivation in cultured astrocytes

Trophic deprivation contributes to astrocyte damage that occurs in acute and chronic neurodegenerative disorders. Unraveling the underlying mechanisms may pave way to novel cytoprotective strategies. Cultured mouse astrocytes responded to trophic deprivation with a large and transient increase in the expression of p21(ras), which was secondary to an enhanced formation of reactive oxygen species (ROS) detected by cytofluorimetric analysis after preloading with 2',7'-dichlorofluorescein diacetate. The increase in p21(ras) levels was largely attenuated by the reducing agent, N-acetylcysteine, which was proven to reduce ROS formation in astrocytes subjected to serum deprivation. We extended the analysis to the Ha-Ras isoform, which has been implicated in mechanisms of cytotoxicity. We found that serum deprivation enhanced the expression and activity of Ha-Ras without changing Ha-Ras mRNA levels. The increase in Ha-Ras levels was sensitive to the protein synthesis inhibitor, cycloheximide, suggesting that serum deprivation increases translation of preformed Ha-Ras mRNA. The late decline in Ha-Ras levels observed after 60 min was prevented by the proteasome inhibitor, MG132, as well as by the selective mitogen-activated protein kinase (MAPK) inhibitor, PD98059. Serum deprivation led to the activation of the MAPK pathway in cultured astrocytes, as shown by an increase in phosphorylated extracellular signal-regulated kinase 1/2 levels after 5 and 30 min. Finally, using the siRNA technology, we found that an acute knock-down of Ha-Ras was protective against astrocyte damage induced by serum deprivation. We conclude that cultured astrocytes respond to trophic deprivation with an increased expression in Ha-Ras, which is limited by the concomitant activation of the MAPK pathway, but is nevertheless involved in the pathophysiology of cell damage

Liquid-state NMR spectroscopy for complex carbohydrate structural analysis: A hitchhiker's guide

: Structural determination of carbohydrates is mostly performed by liquid-state NMR, and it is a demanding task because the NMR signals of these biomolecules explore a rather narrow range of chemical shifts, with the result that the resonances of each monosaccharide unit heavily overlap with those of others, thus muddling their punctual identification. However, the full attribution of the NMR chemical shifts brings great advantages: it discloses the nature of the constituents, the way they are interconnected, in some cases their absolute configuration, and it paves the way to other and more sophisticated analyses. The purpose of this review is to provide a practical guide into this challenging subject. It will drive through the strategy used to assign the NMR data, pinpointing the core information disclosed from each NMR experiment, and suggesting useful tricks for their interpretation, along with other resources pivotal during the study of these biomolecules