Strategies for estimating human exposure to mycotoxins via food

In this review, five strategies to estimate mycotoxin exposure of a (sub-) population via food, including data collection, are discussed with the aim to identify the added values and limitations of each strategy for risk assessment of these chemicals. The well-established point estimate, observed individual mean, probabilistic and duplicate diet strategies are addressed, as well as the emerging human biomonitoring strategy. All five exposure assessment strategies allow the estimation of chronic (long-term) exposure to mycotoxins, and, with the exception of the observed individual mean strategy, also acute (short-term) exposure. Methods for data collection, i.e. food consumption surveys, food monitoring studies and total diet studies are discussed. In food monitoring studies, the driving force is often enforcement of legal limits, and, consequently, data are often generated with relatively high limits of quantification and targeted at products suspected to contain mycotoxin levels above these legal limits. Total diet studies provide a solid base for chronic exposure assessments since they provide mycotoxin levels in food based on well-defined samples and including the effect of food preparation. Duplicate diet studies and human biomonitoring studies reveal the actual exposure but often involve a restricted group of human volunteers and a limited time period. Human biomonitoring studies may also include exposure to mycotoxins from other sources than food, and exposure to modified mycotoxins that may not be detected with current analytical methods. Low limits of quantification are required for analytical methods applied for data collection to avoid large uncertainties in the exposure due to high numbers of left censored data, i.e. with levels below the limit of quantification

Fourier series for eclipses on exoplanet binaries

A double planet system or planet binary undergoes eclipses that modify the reflective light curve. In the time domain, the eclipse events are fast and weak. This would make their signal difficult to find and recognize in the phase light curve, even for small inclinations when eclipses happen frequently. However, due to the quasiperiodic nature of the phenomenon, the Fourier transform of the direct reflection signal consists of a double sum of sharp peaks. These peaks can be resolved for large close binaries and sufficiently long observation times with a star coronagraph. Eclipses modulate the phase curve, having an orbital period $2\pi/\omega$, with a contribution from the relative motion in the binary plane of a period $2\pi/\Omega$. This leads to a spectral structure with basis frequencies $\omega$ and $\Omega$. We aim to characterize these spectra. We studied the regime of short eclipses that occur when the planet radii are small compared to the planet separation. We derived formulas for the peak amplitudes applicable to homogeneous (Lambertian) planet binaries in circular orbit with small inclination. The effects of an eclipse and of double reflection appear as first- and second-order contributions (in planet radius over separation) in the reflection signal respectively. Small peaks appear as observable side bands in the spectrum. Identical structures around $m\Omega$ are characteristic of short-duration eclipses. Deceasing side bands could indicate double reflection between companions. Fourier analysis of the light curve of non-transiting planets can be used to find planets and their moons. Difficulties in interpreting the structures arise for small planet separation and when there are several moons in mean-motion resonance.Comment: 12 pages, 10 figure

A rapid procedure for the extraction of genomic DNA from intact Aspergillus spores

Genomic DNA of different species of Aspergillus was prepared from intact spores using the Nucleon MiY kit of Amersham. The method is rapid, does not involve mechanical disruption of the spores nor the use of phenol-chloroform extractions and yields DNA that is suitable for PCR amplification and Southern analysis. The method is also applicable to mycelium ground with glass beads