32 research outputs found
Efecto de el extracto dializable de leucocitos Bovino (EDLb) en el shock endotóxico inducido por Lipopolisácaridos
[Tesis] (Doctor en Ciencias con Especialidad en Microbiología) U.A.N.L.UANLhttp://www.uanl.mx
Air Pouch Model: An Alternative Method for Cancer Drug Discovery
The tumor microenvironment (TME) is composed of cancer, immune, and stromal cells that interact through cell-to-cell contact and a diverse milieu of cytokines, chemokines, growth factors, and proteases. Several reports have linked the presence of specific cell subtypes with tumor stages, prognosis, and patient survival. Understanding cancer cell behavior and their response to treatment within the tumor microenvironment is essential to prevent establishment, growth, and progression of tumors. Many synthetic and biological agents have been tested using cell-based assays, which do not provide reliable predictive capacity for drug candidate performance in vivo. In this chapter, we discuss about the benefits of an air pouch tumor model, in which tumor cells are inoculated inside an air pouch created on the back of the mouse. The air pouch cancer model serves as a confined/localized tumor microenvironment, where direct contact of drug candidates and tumor cells is guaranteed in a tumor microenvironment context. Therefore, the efficacy of the therapeutic agent can be accurately assessed in vivo
Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis
Lymphotactin-XCL1 is a chemokine
produced mainly by activated CD8? T-cells and
directs migration of CD4? and CD8? lymphocytes
and natural killer (NK) cells. We expressed human
lymphotactin (LTN) by the lactic-acid bacterium
Lactococcus lactis. Biological activity of LTN was
confirmed by chemo-attraction of human T-cells by
chemotaxis demonstrating, for the first time, how this
chemokine secreted by a food-grade prokaryote
retains biological activity and chemoattracts T lymphocytes.
This strain thus represents a feasible
well-tolerated vector to deliver active LTN at a
mucosal level