Lymphotactin-XCL1 is a chemokine
produced mainly by activated CD8? T-cells and
directs migration of CD4? and CD8? lymphocytes
and natural killer (NK) cells. We expressed human
lymphotactin (LTN) by the lactic-acid bacterium
Lactococcus lactis. Biological activity of LTN was
confirmed by chemo-attraction of human T-cells by
chemotaxis demonstrating, for the first time, how this
chemokine secreted by a food-grade prokaryote
retains biological activity and chemoattracts T lymphocytes.
This strain thus represents a feasible
well-tolerated vector to deliver active LTN at a
mucosal level
