Kajian Kesesuaian Poli(2-Hidroksietil Metakrilat-Ko-Metil Hetakrilat) Sebagai Penyokong Penyekatgerakan Lipase

Lipase (Triasilgli serolester hidrolas e,E.C.3.1.1.3) dari Candida cylindracea di sekat gerak secara penjerapan pada penyokong polimer. Polimer ini di sintesis secara pempolimeran ampaian dua monomer, 2-hidroksietil metakrilat (HEMA) dan metil meta krilat (MMA)pada komposisi yang berbeza l:2, 1:1, 2:1, mol/mol). Etilena glikol dimetakrilat (EGDMA)dan divinil benzena (DVD) pada peratusan yang berbeza (w/w)digunakan sebagai agen rangkai silang. Kesan kandungan awal air dan saiz zarah-zarah polimer (180, 180-350, 350-500pm)terhadap keberkesanan proses sekatgerak dikaji berdasarkan kepada tindak balas hidrolisis dan pengesteran. Poli (HEMA-ko-MMA)pada saiz 180-350pm menunjukkan aktiviti paling tinggi bagi kedua-dua aktiviti hidrolisis dan pengesteran

PHYTIC ACID (MYO-INOSITOL HEXAPHOSPHATE)- A PROMISING PHARMACEUTICAL AGENT: A REVIEW

Phytic acid (myo-inositol hexaphosphate) has gained much attention among researchers for its therapeutic value. This review focuses on pharmacological activities of phytic acid, which have been demonstrated by pre-clinical studies. Anti-carcinogenic, antioxidant, antidiabetic, hypolipidemic, anti-angiogenic, and immunomodulation are the most extensively studied pharmacological activities of phytic acid. In addition to these, phytic acid and its derivatives also possess enzyme inhibitory activities against few key enzymes such as 5-alpha reductase, aldose reductase, cyclooxygenase, lipase, matrix metalloproteinase-2 & 9, pepsin, polyphenol oxidase, protease, β-secretase, tyrosinase, and xanthine oxidase were emphasized. The profound pharmacological activities of phytic acid have proven to be a good therapeutic agent and could be employed in preventing as well as treating such diseases

Curcumin combination therapy: the implication and efficacy in cancer

Many chemotherapeutic drugs have been used for the treatment of cancer, forinstance, doxorubicin, irinotecan, 5-fluorouracil, cisplatin, and paclitaxel. However, the effectiveness of chemotherapy is limited in cancer therapy due to drug resistance, therapeutic selectivity, and undesirable side effects. The combination of therapies with natural compounds is likely to increase the effectiveness of drug treatment as well as reduce the adverse outcomes. Curcumin, a polyphenolic isolated from Curcuma longa, belongs to the rhizome of Zingiberaceae plants. Studies from in vitro and in vivo revealed that curcumin exerts many pharmacological activities with less toxic effects. The biological mechanisms underlying the anticancer activity of co-treatment curcumin and chemotherapy are complex and worth to discuss further. Therefore, this review aimed to address the molecular mechanisms of combined curcumin and chemotherapy in the treatment of cancer. The anticancer activity of combined nanoformulation of curcumin and chemotherapy was also discussed in this study. Taken together, a better understanding of the implication and underlying mechanisms of action of combined curcumin and chemotherapy may provide a useful approach to combat cancer diseases

Phytate, iron, zinc and calcium contents and their molar ratios in selected raw and prepared food commonly consumed in Malaysia

Bioavailability is the ability of the body to digest and absorb the mineral in the food consumed. There are several factors that can influence the bioavailability of the food such as oxalate, phytate, acidity of intestinal environment, fibre and also competition with other minerals. The objective of this study is to estimate the inhibitory effect of phytate on the bioavailability of iron, zinc and calcium by measuring their molar ratios. A total of 30 food samples consisting of 12 rice and rice products, 5 wheat and wheat products, 5 grains and cereal based products and 7 different popular varieties of cooked rice and rice products and 1 cooked wheat and wheat products were selected. The samples were purchased from three different supermarkets and shops in Serdang, Selangor. The phytate content in food was analysed using the anion-exchange chromatography followed by spectrophotometry whereas iron, zinc and calcium contents were analysed using atomic absorption spectrophotometry. One-way ANOVA test was used to statistically analyse the mean difference between the phytate and mineral contents and their molar ratios between the food samples. The lowest phytate content in food sample is chicken rice (0.210 ± 0.073 mg/100 g) whereas Quaker Oats has the highest phytate content (19.746 ± 0.985 mg/100 g). The food sample that has the highest content of iron is Quaker Oats (3.567 ± 0.120 mg/100 g) and the lowest is chicken rice (0.123 ± 0.003 mg/100 g). For zinc content, the food sample that has the highest values is Quaker Oats (2.940 ± 0.049 mg/100g) and the lowest is fried kueh-teow (0.126 ± 0.002 mg/100g). The food sample that has the highest calcium content is Cookie Crisps (203.616 ± 16.680 mg/100g) whereas the lowest is kueh-teow (0.262 ± 0.007 mg/100g). Generally results show that the cooked products mostly have lower content of phytate and minerals as compared to the raw products. This could be due to the cooking method that could have influenced the phytate and minerals content in the food. Of the 30 food samples, 24 foods have a phytate/iron ratio > 1.00, 2 foods had phytate/zinc ratio > 15 and 22 foods had phytate/calcium ratio > 0.24. This results show that although many foods analysed have high mineral content, they also contain high phytate that may impair the bioavailability of minerals in the body

Suppression of β-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA)

Background: Phytic acid (PA) is a polyphosphorylated carbohydrate that can be found in high amounts in mostcereals, legumes, nut oil, seeds and soy beans. It has been suggested to play a significant role in inhibition of colorectal cancer. This study was conducted to investigate expression changes of β-catenin and cyclooxygenase-2 COX-2) and cell proliferation in the adenoma-carcinoma sequence after treatment with rice bran PA by immunocytochemistry. Materials and Methods: Seventy-two male Sprague-Dawley rats were divided into 6 equal groups with 12rats in each group. For cancer induction two intraperitoneal injections of azoxymethane AOM) were given at 15 mg/kg bodyweight over a 2-weeks period. During the post initiation phase, two different concentrations of PA, 0.2%(w/v) and 0.5% (w/v) were administered in the diet. Results: Results of ß-catenin, COX-2 expressions and cell proliferation of Ki-67 showed a significant contribution in colonic cancer progression. For ß-catenin and COX-2 expression, there was a significant difference between groups at p<0.05. With Ki-67, there was a statistically significant lowering the proliferating index as compared to AOM alone (p<0.05). A significant positive correlation (p=0.01) was noted between COX-2 expression and proliferation. Total ß-catenin also demonstrated a significant positive linear relationship with total COX-2 (p=0.044). Conclusions: This study indicated potential value of PA extracted from om rice bran in reducing colonic cancer risk in rats

Molecular docking analysis of Carica papaya Linn constituents as antiviral agent

Carica papaya (papaya) fruits are available throughout the world and it is well accepted as food or as a quasi-drug. Aqueous papaya leaves extract have been used as treatment for dengue fever. This prompted us to carry out the docking study on these nine selected ligands (phyto-constituents of papaya) which are carpaine, dehydrocarpaine I and II, cardenolide, p-coumaric acid, chlorogenic acid, caricaxanthin, violaxanthin and zeaxanthin. These phyto-constituents were evaluated on the docking behaviour of dengue serotype 3 RNA-dependent RNA polymerase (RdRp); influenza A (H1N9) virus neuraminidase (NA); chikungunya virus glycoprotein (E3-E2-E1) and chikungunya virus non-structural protein2 (nsP2) protease using Discovery Studio Version 3.1. In addition, molecular physicochemical, drug-likeness, ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicity) and TOPKAT (Toxicity Prediction by Komputer Assisted Technology) analyses were done. The molecular physicochemical analysis revealed that cardenolide and p-coumaric acid (2 ligands) complied with Lipinski’s rule of five. Dehydrocarpaine II, cardenolide, caricaxanthin, violaxanthin and zeaxanthin all the five ligands were predicted to have plasma protein binding (PPB) effect. Docking studies and binding free energy calculations revealed that p-coumaric acid exhibited very least binding energy irrespective of its target protein. Hence, the results of this present study exhibited the potential of these nine ligands as antiviral agent

Water extract of brewers rice induces antiproliferation of human colorectal cancer (HT-29) cell lines via the induction of apoptosis

OBJECTIVE: Brewers' rice, a mixture of broken rice, rice bran, and rice germ, is a rice by-product in the rice industry. The present study was designed to investigate the in vitro cytotoxicity of the water extract of brewers' rice (WBR) against colorectal cancer (HT-29) cells. MATERIALS AND METHODS: The cytotoxicity activity was determined using the lactate dehydrogenase (LDH) assay. The morphological changes of the HT-29 cells were observed using inverted light and fluorescence microscope. Cell cycle and apoptotic cell death analyses were performed using flow cytometer. Besides that, the selected polyphenolic compounds in WBR were also analyzed using ultra performance liquid chromatography (UPLC). RESULTS: The cytotoxicity results showed that WBR was more cytotoxic (but not significantly different) in HT-29 cells compared to the MBR, with IC50 value of 21.88 ± 12.43 µg/mL and 34.50 ± 5.92 µg/mL for WBR and MBR, respectively (p > 0.05). WBR-treated HT-29 cells displayed the typical characteristics of apoptosis, as visualized using inverted light and fluorescence microscope. WBR also significantly increased the number of early and late apoptotic HT-29 cells compared to control cells (p < 0.05). Results from UPLC analysis demonstrated that ferulic acid (36.42 ± 2.97 µg/g) was found the highest level in WBR, followed by gallic acid (26.09 ± 2.01 µg/g) and p-coumaric acid (7.13 ± 0.36 µg/g). These phenolics are speculated to partially contribute to apoptotic cell death. CONCLUSIONS: Our results suggested that WBR derived from natural sources might represent a potential chemopreventive agent against colon cancer

By-products of rice processing: an overview of health benefits and applications

Our study was centred on the increasing literature associated with rice by-products and main components, especially those intended to combat cancer, improve plasma lipid levels or control the blood glucose levels. Rice byproducts, such as rice straw, rice husks, rice bran, rice germ and broken rice, are extensively abundant agricultural wastes from the rice industry, and the percentage of their production depends on the milling rate and type of rice. Among all rice by-products, rice bran has been extensively studied. It contains phytochemicals such as γ-oryzanol, vitamin E, mainly tocotrienols and dietary fibre. This paper reviews the existing literature on the potential role of rice by-products, focusing not only on the role of rice bran but also on the roles of other rice by-products, such as rice germ and rice husk, in the management of the diseases, investigating their various potential uses in the food industry and all possible properties that may contribute to these effects

Manilkara zapota (L.) P. Royen leaf water extract triggered apoptosis and activated caspase-dependent pathway in HT-29 human colorectal cancer cell line

Manilkara zapota (L.) P. Royen (Family: Sapotaceae), commonly called as sapodilla, has been applied as traditional folk medicine for diarrhea and pulmonary infections. Conventional therapy in colorectal cancer is not likely effective due to undesirable outcomes. The anti-colon cancer properties of Manilkara zapota leaf water extract have yet to be investigated thus far. Therefore, our present study aimed to evaluate the ability to induce apoptosis and the underlying mechanisms of Manilkara zapota leaf water extract against human colorectal cancer (HT-29) cells. The cytotoxicity of Manilkara zapota leaf water extract was screened in different cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) analyses. The morphological changes in HT-29 cell lines after exposure to Manilkara zapota leaf water extract were viewed under fluorescence and inverted light microscope. The apoptotic cell was measured by Annexin V-propidium iodide staining. The caspase-3 and -8 activities were assessed by colorimetric assay. Overall analyses revealed that treatment with Manilkara zapota leaf water extract for 72 h can inhibit the viability of HT-29 cells. Incubation with Manilkara zapota leaf water extract for 24, 48, and 72 h significantly increased (p < 0.05) the total apoptotic cells compared to the control. Treatment with 21, 42, and 84 μg/mL of Manilkara zapota leaf water extract for 72 h triggered both caspase-3 and -8 activities in a concentration-dependent pattern. We also found that the catalase level in the two treatment groups (21 and 42 μg/mL) was significantly elevated after 24 h incubation. Incubation with Manilkara zapota leaf water extract for 72 h triggered the transcriptional elevation of the adenomatous polyposis coli (APC), glycogen synthase kinase 3β (GSK3β), AXIN1, and casein kinase 1 (CK1). The β-catenin mRNA levels were reduced accordingly when the concentration of the Manilkara zapota leaf water extract was increased. Our results suggested that Manilkara zapota leaf water extract offer great potential against colorectal cancer through modulation of Wnt/β-catenin signaling pathway, caspase-dependent pathway, and antioxidant enzyme

Brewers' rice, a by-product from rice processing, has antiproliferative activity on human colorectal cancer (HT-29) cell line

Colon carcinogenesis is a malignant tumor, and is well-known as the third leading cancer, which contributes to high mortality and morbidity worldwide. Brewers' rice, known locally as temukut, consists of a mixture of broken kernels with rice bran and rice germ which is a byproduct produced in the rice industry. Although extensive studies on the anti-cancer properties of rice bran, published data on the cytotoxicity of brewers' rice are very limited. The present study was conducted to evaluate the apoptosis induction capability of the water extract of brewers' rice (WBR) on human colorectal cancer (HT-29) cell line. The HT-29 cells were treated with various concentrations (16, 32, and 64 μg/mL) of WBR for 24 and 48 hours. The morphological analysis of apoptotic cells was evaluated using inverted light microscope and fluorescence microscope. The apoptotic HT-29 cells was evaluated using Annexin V-FITC and propidium iodide (PI) staining apoptosis test and cell cycle analyses. The data obtained were evaluated using a one-way analysis of variance (ANOVA) and P < 0.05 was considered statistical significant. Overall analyses indicated that WBR induced typical characteristics of apoptosis in HT-29 cells, including nuclear fragmentation (NF), nuclear compaction (NC), apoptotic bodies (AB), cellular shrinkage (CS), and chromatin condensation (CC), as visualized under inverted light microscope and fluorescence microscope. Cell cycle analyses and Annexin V-FITC and propidium iodide (PI) staining apoptosis test using flow cytometry revealed that WBR induced apoptotic population in HT-29 cells. In this study, our findings provide clear evidence that WBR inhibits the growth of HT-29 cells via induction of apoptosis. Taken together, we suggest that WBR may be a potential candidate for the prevention and treatment of colorectal cancer