Phytochemical screening and antibacterial evaluation of stem bark of Mallotus philippinensis var. Tomentosus

Mallotus philippinensis var. Tomentosus is a medicinal plant, which was tested against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi and Bacillus subtilis. Phytochemicalscreening of the stem bark of M. philippinensis indicates the presence of secondary metabolites. From the results obtained, eluted fractions of chloroform and methanolic extracts showed excellent zone of inhibition comparable to the standard drug used. However, the hexane extract did not show any appreciable activity. The results of the study showed the justification of the use of the plant against the bacterial pathogens

In vivo efficiency of Bacillus sp. isolated from biofloc system on growth, haematological, immunological and antioxidant status of genetically improved farmed tilapia (GIFT)

In aquaculture, Biofloc technology is emerging as one of the successful sustainable technologies to increase production. It is known to possess several immunostimulatory compounds exhibiting possible probiotic effect in culture. Here, we explored the in vivo efficiency of Bacillus infantis (T1), B. subtilis (T2), Exiguobacterium profundum (T3) and B. megaterium (T4) isolated from biofloc systems for improving the growth and immune performance of genetically improved farmed tilapia (GIFT). Animals (10+0.08 g) were stocked at a density of 100 per m-3 in 500 L FRP tanks for 42 days in triplicate. All the four probiotics (OD =1) were mixed with basal diet in treatments and feed without probiotic maintained as control. A significant difference (P <0.05) in weight gain, specific growth rate and FCR were observed between treatments and control with 100% survival. Serum albumin, globulin, protein, total blood count, glucose, myeloperoxidase activity and SOD were significantly different (P <0.05) between treatments and control. T4 and T2 showed better immunological and anti-oxidant ability when compared to other strains. Results from principal component analysis demonstrated that B. megaterium and B. subtilis can be the promising probiotic bacteria isolated from biofloc systems exhibiting multiple benefits with improved growth and health of the culture animals

In vivo efficiency of Bacillus sp. isolated from biofloc system on growth, haematological, immunological and antioxidant status of genetically improved farmed tilapia (GIFT)

714-721In aquaculture, Biofloc technology is emerging as one of the successful sustainable technologies to increase production. It is known to possess several immunostimulatory compounds exhibiting possible probiotic effect in culture. Here, we explored the in vivo efficiency of Bacillus infantis (T1), B. subtilis (T2), Exiguobacterium profundum (T3) and B. megaterium (T4) isolated from biofloc systems for improving the growth and immune performance of genetically improved farmed tilapia (GIFT). Animals (10+0.08 g) were stocked at a density of 100 per m-3 in 500 L FRP tanks for 42 days in triplicate. All the four probiotics (OD =1) were mixed with basal diet in treatments and feed without probiotic maintained as control. A significant difference (P 0.05) in weight gain, specific growth rate and FCR were observed between treatments and control with 100% survival. Serum albumin, globulin, protein, total blood count, glucose, myeloperoxidase activity and SOD were significantly different (P B. megaterium and B. subtilis can be the promising probiotic bacteria isolated from biofloc systems exhibiting multiple benefits with improved growth and health of the culture animals

Renoprotective effect of grape seeds extract in ethylene glycol induced nephrotoxic mice

356-359Grape seed extract treatment in ethylene glycol (EG) induced nephrotoxic mice improved antioxidant status and significantly decreased urinary lactate dehydrogenase (LDH) and lipid peroxidation. The extract rendered antioxidant protection against oxidative stress induced by EG and may help in protecting renal tissue against EG toxicity

Prolonging the shelf life of jasmine (Jasminum grandiflorum) buds by chemical treatments under cold storage condition

Aim of this study is to enhance the shelf life of jasmine flower buds by application of different anti-senescence chemicals to meet out the marketing demand with improved post-harvest quality. The experimental design followed for this study is completely randomized block design with seven treatments, each replicated thrice. The harvested jasmine flowers were treated with different anti-senescence chemicals viz., Silver nanoparticle (SNP) (20ppm), Boric acid (4%), Sucrose (4%), NAA (100 ppm), BA (500 ppm), α-AIB (20µM) and packed in 200 gauge polypropylene bag without ventilation then stored in cold storage condition. Among the different anti-senescence chemical treatments silver nanoparticle (20 ppm) shows positive significant difference in freshness index, flower opening index, color retention index and maintained the shelf life up to 11 days over the control for 7 days

Role of the Campylobacter jejuni Cj1461 DNA Methyltransferase in Regulating Virulence Characteristics▿ †

Mutation of the cj1461 predicted methyltransferase gene reduced the motility of Campylobacter jejuni 81-176. Electron microscopy revealed that the mutant strain had flagella but with aberrant structure. The Δcj1461 mutant was sevenfold more adherent to but 50-fold less invasive of INT-407 human epithelial cells than the wild type