Detection of Gene Involved in Biofilm Formation in Methecillin Resistance Staphylococcus aureus and Staphylococcus lentus and Effect of Camphor extract on These Genes

This research aimed to investigate the antibacterial activity of Camphor extract ,against biofilm formation of clinical isolates Staphylococcus aureus and Staphylococcus lentus .by detecting the presence and absence of virulence genes that responsible for the biofilm formation after treatment  with Camphor  extract. .thirty isolates were diagnosed initially as Staphylococci then selected five isolates depending on resistance to different type of antibiotics as virulence .After that the isolates test by VITEK-2compact system (ID and AST) to confirm the species of Staphylococci .The diagnostic result showed that three isolates belong to Staphylococcus aureus and two to Staphylococcus lentus. Investigation of the ability of camphor oil to inhibit the growth of bacteria using diffusion method in Muller Hinton agar ,the results showed the effect of camphor oil to prevent biofilm formation  on both bacterial species with concentration (0.1 g/ml )

Evaluation of tumor necrosis factor alpha and interlukine-11 in a sample of aborted women infected with human Cytomegalovirus

This study aimed to evaluate the immunological role of TNF-alpha and interlukine-11 for a sample of women infected with HCMV. 65 blood samples were collected from aborted women and 15 blood samples were collected from healthy non-aborted women (as a control group) during the period from 6 April to 12 June from reviews Al-Zahra teaching hospital and Al-Manathira general hospital in Najaf governorate. HCMV IgM, IgG antibody tests were performed by using Enzyme-Linked Immunosorbent Assay (ELISA- Human, Germany).The results showed that the prevalence of IgG and IgM immunoglobulins against HCMV in pregnant women with miscarriages according to the statistical analysis that there is a high significantly difference with a level ≤ P 0.05 in IgG and IgM concentration within different age groups of aborted women compared to the control treatment group of women.The samples under study were also tested to measure the level of TNF-α and IL-11 cytokines by using a quantitative technique the enzyme-linked immunosorbent assay as well, the results showed that there is a hight significant in the concentration of TNF-α protein in aborted women, which reached 8.24 ± 1.58 (pg/ml)., and this was significant at P ≤ 0.003 compared with the control group 5.51 ± 2.96(pg/ml)

Serological study of human Cytomegalovirus spread among aborted women in Najaf Governorate and assessment of MCP-1 chemokine effect on the virus

This study aims to determine the prevalence of IgM, IgG, anti-Human cytomegalovirus (HCMV) in Najaf women and determining the level of MCP-1 chemokine concentration and its role in the immune response. This study was conducted on 65 were suffering from HCMV infection and were referred to the women's consulting clinic and maternity halls in each of the Al-Zahra teaching hospital and Al-Manathira general hospital and 15 women as a control without any miscarriage, during the period from 6 April to 12 June, and these samples were chosen within the age range is between (18 to 40) years. HCMV IgM, IgG antibody tests were performed using Enzyme-Linked Immunosorbent Assay, and the result showed that the prevalence of IgG and IgM immunoglobulins in pregnant women with miscarriages according to the statistical analysis that there is a high significantly difference with a level ≤ P 0.05 in IgG and IgM concentration within different age groups of aborted women compared to the control treatment group of women.&nbsp