The Drosha rs10719 T>C polymorphism is associated with preeclampsia susceptibility

Purpose: Drosha is a member of the micro RNA (miRNA) processing machinery that affects miRNA processing. Single-nucleotide polymorphisms (SNPs) in the Drosha gene might affect microRNA processing and the expression of various genes. The aim of this study is to investigate the association between SNPs in the Drosha gene and preeclampsia (PE) in the southeast of Iran. Methods: Genotyping of Drosha rs10719 and rs6877842 was performed using blood samples from 219 PE women and 205 healthy control subjects by a polymerase chain reaction-restriction fragment length polymorphism method. Results: The Drosha rs10719TC genotype was significantly associated with 1.6-fold higher risk of PE (odds ratio (OR, 1.6 95% CI, 1.1�2.4, P = 0.026). In addition, the frequency of the Drosha rs10719CC genotype was significantly higher in PE women and was associated with threefold higher risk of PE (OR 3 95% CI 1.4�6.3, P = 0.004). There was no association between the Drosha rs6877842 polymorphism and PE susceptibility. The CC�GG combined genotype was associated with 3.4-fold higher risk of PE (OR 3.4 95% CI 1.4�8.1, P = 0.007). The haplotype-based association analysis showed higher frequency of C�G haplotype of Drosha rs10719 and rs6877842 polymorphisms with the increased risk of PE 1.5-fold (OR 1.5 95% CI 1.1�2, P = 0.01). Conclusions: The Drosha rs10719TC and CC genotypes were associated with PE risk. The CC�GG combined genotype and C�G haplotype of Drosha rs10719 and rs6877842 polymorphisms may increase PE susceptibility. © 2018 Taylor & Francis

The Drosha rs10719 T>C polymorphism is associated with preeclampsia susceptibility

Purpose: Drosha is a member of the micro RNA (miRNA) processing machinery that affects miRNA processing. Single-nucleotide polymorphisms (SNPs) in the Drosha gene might affect microRNA processing and the expression of various genes. The aim of this study is to investigate the association between SNPs in the Drosha gene and preeclampsia (PE) in the southeast of Iran. Methods: Genotyping of Drosha rs10719 and rs6877842 was performed using blood samples from 219 PE women and 205 healthy control subjects by a polymerase chain reaction-restriction fragment length polymorphism method. Results: The Drosha rs10719TC genotype was significantly associated with 1.6-fold higher risk of PE (odds ratio (OR, 1.6 95% CI, 1.1�2.4, P = 0.026). In addition, the frequency of the Drosha rs10719CC genotype was significantly higher in PE women and was associated with threefold higher risk of PE (OR 3 95% CI 1.4�6.3, P = 0.004). There was no association between the Drosha rs6877842 polymorphism and PE susceptibility. The CC�GG combined genotype was associated with 3.4-fold higher risk of PE (OR 3.4 95% CI 1.4�8.1, P = 0.007). The haplotype-based association analysis showed higher frequency of C�G haplotype of Drosha rs10719 and rs6877842 polymorphisms with the increased risk of PE 1.5-fold (OR 1.5 95% CI 1.1�2, P = 0.01). Conclusions: The Drosha rs10719TC and CC genotypes were associated with PE risk. The CC�GG combined genotype and C�G haplotype of Drosha rs10719 and rs6877842 polymorphisms may increase PE susceptibility. © 2018 Taylor & Francis

Effects of cigarette smoke and opium on the expression of CD9, CD36, and CD68 at mRNA and protein levels in human macrophage cell line THP-1

Cigarette smoking and opium use are risk factors for coronary artery disease (CAD). It has been known that scavenger receptors such as CD36 and CD68 play critical roles in the pathogenesis of CAD. CD9, as a member of the tetraspanin, has been shown to interact with scavenger receptors. The aim of this study was to investigate the effects of these risk factors on expression levels of CD9, CD36, and CD68 on the THP-1 cell line. The THP-1 cell line treated with cigarette smoke extract (CSE) and opium, both individually and combinatory, in 24 h incubation. The protein and mRNA levels of CD9, CD36, and CD68 were evaluated by flow cytometry and quantitative reverse transcription-Polymerase Chain Reaction (qRT-PCR) techniques, respectively. CD36 and CD68 mRNA and protein expression levels were significantly increased in the cells treated with cigarette smoke extract compared to the control (p<0.001 in mRNA expression levels and p=0.016 and p=0.012 in protein expression levels, respectively). The CSE increased the level of CD9 protein expression compared to the control group (p=0.041) on the human macrophage cell line THP-1. No significant differences were observed in the CD9, CD36, and CD68 gene expression and at the protein levels between opium-treated THP-1 cells and controls. Copyright © February 2020, Iran J Allergy Asthma Immunol. All rights reserved

El clima organizacional y su relación con la gestión institucional en la Institución Educativa N° 5003 - Virgen de la Inmaculada Concepción - Callao

Universidad Nacional de Educación Enrique Guzmán y Valle. Escuela de Posgrado.TesisEn la investigación titulada El clima organizacional y su relación con la gestión institucional en la Institución Educativa N° 5003 - Virgen de la Inmaculada Concepción – Callao, el clima organizacional de un grupo social es una variable mediadora entre los propósitos y los logros del mismo grupo. En nuestro país nos encontramos en el proceso de puesta en marcha de la Reforma Educativa, esto significa que los programas de estudio, las metodologías, los recursos materiales y humanos están recibiendo un impulso renovador de acuerdo al nuevo modelo educativo. Desde La perspectiva de una investigación de tipo -descriptivo- explicativo utilizando el diseño correlacional; se llevó a cabo un conjunto de actividades utilizando los conceptos teóricos básicos de clima organizacional y gestión institucional, a través de muestreo no probabilístico se eligió una muestra de 25 integrantes de la Institución Educativa 5003 Virgen de la Inmaculada Concepción, a quienes se les aplicó mediante la técnica de la encuesta y su instrumento el cuestionario, para determinar si el clima organizacional se relaciona con la gestión institucional. La contribución que se hace y se ha encontrado que, si hay una relación significativa, porque tomó situaciones reales propias y se profundizó en el quehacer educativo, que permitió describir, observar, sistematizar e interpretar la información recogida a través de los análisis cuantitativo y cualitativo; además se integró la información para emitir conclusiones importantes. Como resultado de la investigación se puede afirmar que existe una relación entre el clima organizacional y la gestión institucional de esta institución del Callao

The effect of the placental DROSHA rs10719 and rs6877842 polymorphisms on PE susceptibility and mRNA expression

Evidence showed that microRNA biosynthesis plays the main role in pathogenesis of several diseases including Preeclampsia (PE). Therefore, microRNA processing enzymes may involve in PE predisposition. The aim of the present study was to evaluate the relation between DROSHA rs10719 and rs6877842 polymorphisms and mRNA expression in the placenta of PE women and controls. This study recruited 110 PE women and 115 age matched normotensive pregnant women for genotyping of DROSHA polymorphisms and analyzing of mRNA expression. There was no association between alleles and genotypes of placental DROSHA rs10719 and rs6877842 polymorphisms and PE susceptibility. However, placental DROSHA rs10719 was associated with increased PE risk in the recessive model. The combination of CC/GG genotypes of DROSHA rs10719 and rs6877842 polymorphisms was associated with higher risk of PE. The frequency of C-G haplotype was higher in PE women, but the difference was not significant. The DROSHA mRNA expression was downregulated in the placenta of PE women. There was no relation between DROSHA mRNA expression and rs6877842 polymorphism, however, it was decreased in the placenta of women with rs10719CC genotype. The placental DROSHA rs10719 but not rs6877842 polymorphism could be a risk factor for PE susceptibility only in the recessive model. The combination of CC/GG genotypes could be risk factors for PE susceptibility. The DROSHA expression downregulated in the preeclamptic placentas and those carrying rs10719CC genotype. © 2019, Springer Nature Limited