2 research outputs found
Preparation and evaluation of niosomes containing autoclaved Leishmania major: a preliminary study
In this study, different positively charged niosomal formulations containing sorbitan esters, cholesterol
and cetyl trimethyl ammonium bromide were prepared by film hydration method for the entrapment of
autoclaved Leishmania major (ALM). Size distribution pattern and stability of niosomes were investigated by
laser light scattering method and ALM encapsulation per cent was measured by the bicinchoninic acid
method. Finally, the selected formulation was used for the induction of the immune response
against cutaneous leishmaniasis in BALB/c mice. Size distribution curves of all the formulations followed
a log-normal pattern and the mean volume diameter was in the range 7.57–15.80 mm. The mean volume
diameters were significantly increased by adding Tween to Span formulations (p50.05). The percentage of
ALM entrapped in all formulations varied between 14.88% and 36.65%. In contrast to ALM, in vivo studies
showed that the niosomes containing ALM have a moderate effect in the prevention of cutaneous
leishmaniasis in BALB/c mice