Clinical data of patients diagnosed with colon cancer via colonoscopy.

<p>Clinical data of patients diagnosed with colon cancer via colonoscopy.</p

Human beta defensin (hBD) protein expression in colon cancer tissues.

<p>Tissues were immunostained using specific hBD antibodies (Panel 2A). hBD- positive cells in the tissues were estimated as follows: 0 points, no positive color; 1 point, <20% positive staining; 2 points, 21‑50% positive staining; 3 points, 51–75% positive staining; and 4 points, >75% positive staining. This is presented in Panel B.</p

Differents structures of hBDs: hBD-1 (panel A), hBD2 (Panel B), and hBD3mutant and wild type (panel C and D).

<p>Differents structures of hBDs: hBD-1 (panel A), hBD2 (Panel B), and hBD3mutant and wild type (panel C and D).</p

Predicted effect of the mutations affecting hBD-3 on protein structure stability.

<p>^apredicted protein thermal stability change (∆∆G in Kcal/mol) of mutation from CUPSAT program.</p><p>^brelative solvent accessibility of the wild type residue computed from PoP MuSiC program.</p><p>^cpridected protein stability change (∆∆G in Kcal/mol) of mutation from PoP MuSiC program.</p><p>Predicted effect of the mutations affecting hBD-3 on protein structure stability.</p

Summary of hBDs mutations and their nature/location found in colon cancer tissues.

<p>N = Normal colon tissue</p><p>T = Tumor</p><p>Summary of hBDs mutations and their nature/location found in colon cancer tissues.</p

Amino acid sequence alignment of four human beta defensins (panel A for hBD1, panel B for hBD2,Panel C for hBD3 and panel D for hBD4) with the amino acid sequences for their corresponding observed mutants.

<p>Mutations are highlighted in red for each hBDs gene.</p

Human beta defensin (hBD) mRNA and protein expression in colon cancer tissues.

<p>Total cellular RNA freshly extracted from normal and colon cancer tissues was reverse transcribed into cDNA and then used to measure the hBD mRNA expression (Panel1A to 1D).</p