The Inhibitory Effects of Ascorbic Acid, α-Tocopherol, and Sodium Selenite on Proliferation of Breast Cancer Cell Lines

Abstract The role of antioxidants in prevention and treatment of cancers have been reported by several studies. In our investigation we studied the effects of ascorbic acid, α-tocopherol, and sodium selenite on proliferation of two breast cancer cell lines: T47D (estrogen-receptor positive) and MDA-MB-231 (estrogen-receptor negative). We also used 17-β-estradiol as positive control for proliferation of T47D cells. The viability of cells after 7 days of exposure to different concentrations of test compounds was determined by resazurine based method. Ascorbic acid and α-tocopherol significantly inhibited cell growth at a concentration of 10 -4 M in both cell lines and antagonized the cell proliferation induced by 17-β-estradiol in T47D cells. Sodium selenite at concentrations above 10 -6 M strongly inhibited the cell growth in both cell lines and suppressed the stimulated growth of T47D cells by 17-β-estradiol. Our results with different strengths of activity of test compounds, further confirmed the findings of previous studies that showed the inhibitory effects of these antioxidants on other malignant cell lines

Causes and Results of Eye Removal Surgery in Yazd, Iran

Introduction: Removing eyes has different indications. We surveyed the causes and results of eye removal surgery in Yazd, Iran. Methods: We retrospectively reviewed the profile of 102 patients who had underwent eye removal surgery for any reason in the Shahid Sadoughi hospital during the last 10 years. The demographic characteristics, causes of enucleation, the interval between an accident and an eye surgery, and postoperative complications were analyzed. Results:  In 102 patients (males, 68.6% and females, 31.4%) most cases were laborers (21.6%).The two most common causes of eye enucleation were  trauma (60.9%) and painful blind eye (15.7%). Trauma was the most frequent cause in the age group of  ≤20 years old (78.9%) (P-value< 0.001). In 30.4% of the cases, our management was Sympathetic ophthalmia prevention. Conclusion: The first cause of eye removal surgery in our study was trauma, especially in the young men. To reduce the rate of this surgery, protective proceeding, early diagnosis, and treatment of many diseases will be useful

Association between hypertension and insulin resistance in non-diabetic adult populations: a community-based study from the Iran

Background and objectives: High blood pressure increases the probability of insulin resistance and hyperinsulinemia. Also, insulin resistance can be defined as a risk factor for hypertension. The present study investigated the relationship between hypertension and insulin resistance in non-diabetic participants who were referred to Qazvin Metabolic Diseases Center. Material and methods: In this cross sectional study, 1103 participants (111 non-diabetic with newly diagnosed hypertension and 992 normotensive subjects aged ≥ 20 years) were enrolled from September 2014 to April 2016 in Qazvin (Iran). Systolic and diastolic blood pressure, insulin resistance, waist circumference, body mass index, triglycerides, cholesterol, LDL-cholesterol, fasting blood glucose (FBG) were measured. Fasting triglyceride to highdensity lipoprotein cholesterol ratio (TG/HDL-C) was used as a surrogate of insulin resistance. Data were analyzed using SPSS software and p &lt; 0.05 was assumed as significant level. Results: Ten percent of all participants had hypertension. TG/HDL-C was 3.78 ± 3.28 in non-HTN and 5.76 ± 5.35 in HTN participants (p &lt; 0.001). The frequency of all cardio-metabolic risk factors (except HDL cholesterol level) was higher in hypertensive group, after adjusting for age and gender (p &lt; 0.001). Conclusion: Based on these results, essential hypertension is associated with a higher prevalence of hyperinsulinemia and insulin resistance in the non-diabetic community in Iran

The Contents of Sesamol in Iranian Sesame Seeds

Abstract Sesamol is a sesame lignan. Sesame lignans have multiple functions, including antioxidant activity and also seem to have potential as a source of phytostrogens. This study was condncted to evaluate sesamol contents of 7 brands of Iranian sesame seeds (Sesamum indicume L.). The brands were named Karaj 29, Darab 14, Ultan, Dezful, Varamin, Branching Naz, and Nonbranching Naz. After method validation, the methanolic extracts of seeds were investigated by HPLC. Their mean of total sesamol was found to be 4.67±0.92 mg/g (ranging between 2.75 and 6.13 mg/g). The brands Karaj 29 with 5.84±0.25 mg/g, Dezful with 5.48±0.08 mg/g, and Varamin with 5.4±0.1mg/g had the highest content, and Darab 14 with the content of 3.30±0.57mg/g had the lowest (p&lt;0.05). Iranian sesame can be considered to be a good source of natural antioxidants for medicinal and commercial uses

Determination of carbamazepine in urine and water samples using amino-functionalized metal–organic framework as sorbent

Abstract A stable and porous amino-functionalized zirconium-based metal organic framework (Zr-MOF-NH2) containing missing linker defects was prepared and fully characterized by FTIR, scanning electron microscopy, powder X-ray diffraction, and BET surface area measurement. The Zr-MOF-NH2 was then applied as an adsorbent in pipette-tip solid phase extraction (PT-SPE) of carbamazepine. Important parameters affecting extraction efficiency such as pH, sample volume, type and volume of eluent, amount of adsorbent, and number of aspirating/dispensing cycles for sample solution and eluent solvent were investigated and optimized. The best extraction efficiency was obtained when pH of 100 µL of sample solution was adjusted to 7.5 and 5 mg of the sorbent was used. Eluent solvent was 10 µL methanol. Linear dynamic range was found to be between 0.1 and 50 µg L−1 and limit of detection for 10 measurement of blank solution was 0.05 µg L−1. This extraction method was coupled to HPLC and was successfully employed for the determination of carbamazepine in urine and water samples. The strategy combined the advantages of fast and easy operation of PT-SPE with robustness and large adsorption capacity of Zr-MOF-NH2

Antioxidant Capacity of Plasma after Pomegranate Intake in Human Volunteers

Dietary antioxidants including phenolic compounds are believed to be effective nutrients in the prevention of oxidative stress related disease. Pomegranate has been used for centuries in ancient cultures for its medicinal purpose and is widely acknowledged for antioxidant properties. The present study was designed to assess the effect of pomegranate fresh fruit consumption on the plasma antioxidant capacity. Thirty healthy volunteers were recruited for the study. Volunteers were randomly divided into three groups (pomegranate, vitamin E and water consumption). Blood samples were collected, after at least 12 hours overnight fast, the day before beginning supplementation period and the day after supplementation had finished. Total antioxidant capacity measurement by FRAP method and clinical laboratory test were performed for all volunteers in two selected times. The obtained data revealed that consumption of 100 grams pomegranate and vitamin E per day for ten days resulted in a significant rise (14.05%, 8.28%) plasma antioxidant capacity respectively, but this difference was not significant for water group

Noninvasive Fuhrman grading of clear cell renal cell carcinoma using computed tomography radiomic features and machine learning

To identify optimal classification methods for computed tomography (CT) radiomics-based preoperative prediction of clear cell renal cell carcinoma (ccRCC) grade

Association between hypertension and insulin resistance in non-diabetic adult populations: A community-based study from the Iran

Background and objectives: High blood pressure increases the probability of insulin resistance and hyperinsulinemia. Also, insulin resistance can be defined as a risk factor for hypertension. The present study investigated the relationship between hypertension and insulin resistance in non-diabetic participants who were referred to Qazvin Metabolic Diseases Center. Material and methods: In this cross sectional study, 1103 participants (111 non-diabetic with newly diagnosed hypertension and 992 normotensive subjects aged ≥ 20 years) were enrolled from September 2014 to April 2016 in Qazvin (Iran). Systolic and diastolic blood pressure, insulin resistance, waist circumference, body mass index, triglycerides, cholesterol, LDL-cholesterol, fasting blood glucose (FBG) were measured. Fasting triglyceride to highdensity lipoprotein cholesterol ratio (TG/HDL-C) was used as a surrogate of insulin resistance. Data were analyzed using SPSS software and p < 0.05 was assumed as significant level. Results: Ten percent of all participants had hypertension. TG/HDL-C was 3.78 ± 3.28 in non-HTN and 5.76 ± 5.35 in HTN participants (p < 0.001). The frequency of all cardio-metabolic risk factors (except HDL cholesterol level) was higher in hypertensive group, after adjusting for age and gender (p < 0.001). Conclusion: Based on these results, essential hypertension is associated with a higher prevalence of hyperinsulinemia and insulin resistance in the non-diabetic community in Iran

Simultaneous Measurement of Zinc, Copper, Lead and Cadmium in Baby Weaning Food and Powder Milk by DPASV

Abstract Apart from the breast milk, infant formula and baby weaning food have a special role in infant diet. Infants and young children are very susceptible to amount of trace elements. Copper and zinc are two elements that add in infant food. Lead and cadmium are heavy metals that enter to food chain unavoidably. DPASV is a benefit and applicable method for measurement of trace elements in food products. In this study, concentration of zinc, copper, lead and cadmium in four brands of baby food (rice and wheat based) and powder milk was analyzed with DPASV and polarograph set. Total Mean ± SE of zinc, copper, lead and cadmium in baby foods (n = 240) were 11.86 ± 1.474 mg/100g, 508.197 ± 83.154 µg/100g, 0.445 ± 0.006, 0.050 ± 0.005 mg/Kg respectively. Also these amount in powder milk (n = 240) were 3.621± 0.529 mg/100g, 403.822 ± 133.953 µg/100g, 0.007 ± 0.003, 0.060 ± 0.040 mg/Kg respectively. Zinc level in baby food type I was higher than lablled value (P = 0.030), but in other brands was not difference. Concentration of copper in all of samples was in labeled range (P &gt; 0.05). In each four products, level of lead and cadmium were lower than the standard limit (P &lt; 0.05). Amount of zinc and lead in baby food I, had difference versus other products. Concentration of zinc, camium in baby food type I, was higher than type II (P = 0.043, 0.001 respectively). Concentration of lead and cadmium in baby food type II, was higher than infant formulas, but are in standard limit

Evaluation of Antioxidants in Bone Mineral Density of Iranian

AbstractObjective(s) Bone is a dynamic tissue that is continuously renewed throughout life by the process of bone remodeling. Antioxidant system might be involved in the pathogenesis of bone loss, so the aim of this study was to evaluate the total antioxidant capacity (TAC), vitamin C and vitamin E levels of plasma besides measuring enzymatic antioxidants, superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) enzymes activity in Iranian osteoporotic women comparing to the control group.Materials and MethodsBone mineral density (BMD) of the femoral neck and lumbar spine was measured by dual x-ray absorptiometry. The participants were divided into groups: a) total participants (-3.9 ≤ T–score ≤ 3.6) including 192 women, b) the control group (T-score ≥ -1) including 76 women, c) the total patients (T-score < -1) including 76 women. Then, plasma TAC, vitamin C levels, SOD and GR activities, erythrocyte CAT were measured using spectrophotometrical methods separately, and for vitamin E by HPLC analysis.ResultsComparing the control group and osteoporotic women showed that: a) plasma levels for vitamin C and erythrocyte CAT were markedly lower in the patients than in the controls, but plasma activity of TAC, SOD and GR were significantly higher, respectively. b) the differences were higher between control and patients with severe disease (T-score <-1.7) comparing to patients in the group with milder disease (-1.7 ≤ T-score <-1). c) Femoral neck BMD adjusted with age and BMI showed a positive and significant correlation with plasma levels of vitamin C in all subjects, but this relation was reverse or negative for TAC, SOD and GR.ConclusionIt seems that a physiologic increase in the amount of some antioxidants occurs in osteoporosis; even though this amount may not be sufficient for the human body requirements