25.当院における内視鏡的乳頭切開術の臨床的検討(第617回千葉医学会例会・第1内科教室同門会例会)

Primer sequences and amplicon sizes. (DOCX 15 kb

Beyond the Naked Eye

This image illuminates the contours and difference in densities of a seemingly contourless and homogeneously uniform hydrogel. The image is a fluorescence composite of three wavelengths that allow for the boundaries and area within the hydrogel to differ from the featureless transparent image seen by the naked eye. My research uses fluorescence spectroscopy to probe the internal structure of the hydrogel to see how it influences diffusion of biomolecules. These gels at the naked eye level appear to be solid with a defined shape, but taking a deep look at the microscale level reveals the solvent-like properties that permit selective movement of biomolecules. These properties, as well as others, allow hydrogels to be used in a variety of medical applications, such as tissue engineering. Also, as we utilize techniques to provide us with more insight into an image, like the one above, the array of opportunities and applications of the hydrogel will continue to grow

Evaluation of Biomedical Hydrogel Parameters for Biomolecular Diffusion in Controlled Drug Delivery

Hydrogels made of non-toxic poly(ethylene glycol) diacrylate (PEGDA) provide a suitable platform for controlled drug delivery. The effect of the hydrogel’s structural elements on the diffusive nature of therapeutic agent release from PEGDA hydrogels was examined. The system utilized the diffusive nature of biomolecules, such as matrix metalloproteinases (MMPs), to obtain a desired release profile. Hydrogels’ intrinsic physical properties influence the diffusivity of molecules. Thus, manipulation of physical properties by altercations in the structural elements of the gels provided control over the release of therapeutic agents. The microstructure of the hydrogel dictates the gel’s physical properties. Therefore, manipulation of physical properties can be monitored by changes in microstructural properties, such as mesh size. These manipulations were partly accomplished by varying the PEGDA molecular weight. Additionally, methoxy poly(ethylene glycol) monoacrylate (mPEGMA) tethers were incorporated into the hydrogel. The tether length was varied by using different mPEGMA molecular weights. This adds another layer complexity to the gel that can be manipulated in order to tailor it to a specific molecular diffusion profile. To elucidate the changes in small molecule diffusion caused by variations in the different gel components, diffusion was studied using 7-amino-4-methylcoumarin (AMC). The diffusion of AMC through hydrogels was measured using fluorescence spectroscopy in conjunction with a Franz cell apparatus. Also, a bovine serum albumin (BSA) diffusion study was conducted to examine the effects on the diffusion of biomolecules. The BSA diffusion study was completed using the same method as the previous study. This combination of studies and mesh size analysis showed that the diffusion rate is directly proportional to the increase in monomer molecular weight and mesh size but is inversely proportional to tether length

Additional file 1: Figure S1. of Selenium added unripe carica papaya pulp extracts enhance wound repair through TGF-β1 and VEGF-a signalling pathway

Histology of Polymorphonuclear leukocytes (PMNL) and fibroblasts on wound tissue after topical application of Zn2+ or Se2+ added papaya extracts. PMNL (arrow) and fibroblasts (arrow head) at late inflammatory phase i.e., day 4 (A) and wound repair phase i.e., at day 10 (B) post wounding after treatment. At day 10 post wounding. Disrupted dermis with high density of infiltrating inflammatory cells, thin overlying epithelia and lower number of fibroblasts were observed in the negative control (NC) wounds. However, wounds treated with Se2+ (0.5 μg) added papaya PBS and water extracts (PES and WES respectively) exhibited firmly attached epithelium which interdigitate with a more organised dermis, a marked reduction of infiltrating PMNL with significant increased number of fibroblasts as compared to NC and WE. [Scale Bar = 50 μm]. (JPEG 1233 kb

Additional file 1 of Gene selection for cancer classification with the help of bees

Gene Selection for Cancer Classification With the Help of Bees. The supplementary file is available in the link: https://goo.gl/APTj0n . (PDF 417 kb

MOESM1 of Absent words and the (dis)similarity analysis of DNA sequences: an experimental study

Additional file 1. All Distance Matrices. In this file (AllMatrices), all the distance matrices are provided

Additional file 1: Table S1. of Advances in breeding for high grain Zinc in Rice

List of Zinc homeostasis genes and their putative functions Chen et al. 2008; Dräger et al. 2004; Gross et al. 2003; Grotz & Guerinot 2006; Kobayashi et al. 2003; Kobayashi et al. 2005; Koike et al. 2004; Lee et al. 2010a; Lee et al. 2010b; Ricachenevsky et al. 2011; Sasaki et al. 2012; Stein et al. 2009; Takahashi et al. 2012; Vasconcelos et al. 2008; Yang et al. 2007; Yang et al. 2009a; Yang et al. 2009b; Yokosho et al. 2009; Yuan et al. 2012. (DOC 44 kb

Additional file 2: Table S2. of Rad51C-ATXN7 fusion gene expression in colorectal tumors

In silico translation of fusion gene Rad51C-ATXN7. (DOCX 17 kb