Experimental Toxoplasmosis in Rats Induced Orally with Eleven Strains of <i>Toxoplasma gondii</i> of Seven Genotypes: Tissue Tropism, Tissue Cyst Size, Neural Lesions, Tissue Cyst Rupture without Reactivation, and Ocular Lesions - Fig 7

<p>(A) Meningoencephalitis in Rat D6157 infected with the TgCatCo3 strain. Note meningitis (a), perivascular infiltration of leukocytes (b), and a glial nodule with a degenerative tissue cyst (c). PASH stained. (B) A large inflammatory focus, probably resulting from the host reaction to degenerative tissue cysts in Rat D6162 infected with theGT1 strain and HE stained.</p

Experimental toxoplasmosis in rats^a inoculated orally with oocysts of 11 strains of <i>T</i>. <i>gondii</i> of different genotypes.

<p>Experimental toxoplasmosis in rats<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156255#t003fn001" target="_blank">^a</a> inoculated orally with oocysts of 11 strains of <i>T</i>. <i>gondii</i> of different genotypes.</p

Examples of encephalitis with intact and degenerative tissue cysts.

<p>For Rat D6162 infected with the GT1 strain: (A) Vasculitis with five intact tissue cysts at the periphery of the lesion. IHC staining with <i>T</i>. <i>gondii</i> BAG1 antibodies and hematoxylin counterstain. (B) Several glial nodules, one with a degenerative tissue cyst (arrow). (C) Higher magnification of focus arrowed in Fig 8B. Note immunoreactive debris (arrows) in focus of necrosis focus. One intact tissue cyst (arrowhead) is at the periphery of the lesion. (D) The same lesion as in Fig 8B. An intact tissue cyst (arrowhead) is present at the periphery of glial nodule with central necrosis. There are no visible bradyzoites. PASH stain.</p

Multiple necrotizing foci in cerebrum of Rat D6162 infected with the GT1 strain.

<p>Two foci have degenerative tissue cysts (arrows). IHC staining with polyclonal <i>T</i>. <i>gondii</i> antibodies.</p

Measurements of tissue cysts from rat brains infected with different strains of <i>T</i>. <i>gondii</i>.

<p>Measurements of tissue cysts from rat brains infected with different strains of <i>T</i>. <i>gondii</i>.</p

Tissue cyst distribution and pair wise comparison of the brain regions for infected rats.

<p>(A) Box and whisker plot of tissue cyst densities for the regions in the mid-sagittal sections from the 108 rats (top). Table of statistics for comparison between pairs of regions using Mann-Whitney (bottom). SPSS Graph Builder (version 22) was used to draw the boxplot. (B) P-values for comparisons of tissue cyst densities in regions using the Mann-Whitney test. The Bonferroni corrected p-value cutoff for multiple testing is 0.0011 (0.05/45).</p

Summary of studies concerning biology of tissue cysts in rodents.

<p>Summary of studies concerning biology of tissue cysts in rodents.</p

Retinitis in rats.

<p>(A) Inflammatory focus involving the entire width of the retina (a) and encroaching the choroid (b). The sclera (c) is unaffected. Rat D6185 infected with the VEG strain and HE stained. (B) A focally extensive area of inflammation (retinitis) composed of lymphocytes and few macrophages affecting primarily the inner layers of the retina. Rat D6243 infected with the TgBbUS1 strain and HE stained. (C) Four intact tissue cysts (arrows) and a probable degenerating tissue cyst (arrowhead) in retina. Rat D6186 infected with the VEG strain. (D) Tissue cyst (arrow) in retina without host reaction. Rat D6249 infected with the TgBbUS1 strain and HE stained.</p

Focal inflammation in retina (a) of Rat D6143 infected with the TgBbUS1 strain, 7 days p.i.

<p>(A) Necrosis, and inflammatory focus, primarily affecting the inner retinal layers, bulges into the vitreous chamber (arrows). The choroid (b) and sclera (c) are not affected. HE stain. (B) Higher magnification of Fig 2A with two tachyzoites (arrows). (C) A tachyzoite (arrow) in the inflammatory focus. IHC stain with polyclonal <i>T</i>. <i>gondii</i> antibodies. This tachyzoite is larger in size than the tachyzoites stained with HE in Fig 2B because tachyzoites swell during immunostaining.</p

Ocular lesions in rats orally inoculated with <i>T</i>. <i>gondii</i> oocysts.

<p>Ocular lesions in rats orally inoculated with <i>T</i>. <i>gondii</i> oocysts.</p