1 research outputs found
Plasmon-Enhanced Photocleaving Dynamics in Colloidal MicroRNA-Functionalized Silver Nanoparticles Monitored with Second Harmonic Generation
The photocleaving dynamics of colloidal
microRNA-functionalized
nanoparticles are studied using time-dependent second harmonic generation
(SHG) measurements. Model drug-delivery systems composed of oligonucleotides
attached to either silver nanoparticles or polystyrene nanoparticles
using a nitrobenzyl photocleavable linker are prepared and characterized.
The photoactivated controlled release is observed to be most efficient
on resonance at 365 nm irradiation, with pseudo-first-order rate constants
that are linearly proportional to irradiation powers. Additionally,
silver nanoparticles show a 6-fold plasmon enhancement in photocleaving
efficiency over corresponding polystyrene nanoparticle rates, while
our previous measurements on gold nanoparticles show a 2-fold plasmon
enhancement compared to polystyrene nanoparticles. Characterizations
including extinction spectroscopy, electrophoretic mobility, and fluorimetry
measurements confirm the analysis from the SHG results. The real-time
SHG measurements are shown to be a highly sensitive method for investigating
plasmon-enhanced photocleaving dynamics in model drug delivery systems