Single step biotransformation of corn oil phytosterols to boldenone by a newly isolated Pseudomonas aeruginosa

AbstractA new potent Pseudomonas aeruginosa isolate capable for biotransformation of corn oil phytosterol (PS) to 4-androstene-3, 17-dione (AD), testosterone (T) and boldenone (BOL) was identified by phenotypic analysis and 16S rRNA gene sequencing. Sequential statistical strategy was used to optimize the biotransformation process mainly concerning BOL using Factorial design and response surface methodology (RSM). The production of BOL in single step microbial biotransformation from corn oil phytosterols by P. aeruginosa was not previously reported. Results showed that the pH concentration of the medium, (NH4)2SO4 and KH2PO4 were the most significant factors affecting BOL production. By analyzing the statistical model of three-dimensional surface plot, BOL production increased from 36.8% to 42.4% after the first step of optimization, and the overall biotransformation increased to 51.9%. After applying the second step of the sequential statistical strategy BOL production increased to 53.6%, and the overall biotransformation increased to 91.9% using the following optimized medium composition (g/l distilled water) (NH4)2SO4, 2; KH2PO4, 4; Na2HPO4. 1; MgSO4·7H2O, 0.3; NaCl, 0.1; CaCl2·2H2O, 0.1; FeSO4·7H2O, 0.001; ammonium acetate 0.001; Tween 80, 0.05%; corn oil 0.5%; 8-hydroxyquinoline 0.016; pH 8; 200rpm agitation speed and incubation time 36h at 30°C. Validation experiments proved the adequacy and accuracy of model, and the results showed the predicted value agreed well with the experimental values

Combining calligraphy and plant motifs as a starting point for achieving a formative vision of the metal work with the students In the Faculty of Art Education

The issue of Arab identity is a very important issue that always needs study and continuous research in light of the current and future development in various fields of science, arts and literature, which makes us always shed light on it to preserve historical legacies and preserve the Arabic language with its arts and literature, and then preserve the path of the Arab artist and calligrapher and complete the journey of creativity, The field of metalworking is one of the important fields in which the Islamic artist excelled throughout the Islamic ages, integrating in his works writings with decorations. This experiment aimed to identify an aspect of the Islamic and Arab heritage represented in the letters of the thuluth calligraphy, along with some Islamic floral decorations, and the combination of the letters of the thuluth calligraphy and plant decorations to achieve a new vision of metalwork and develop the design ability of students of the College of Art Education to achieve compatibility between special manual plastic methods metal works, And between an important part of the national heritage represented in the Arabic calligraphy and Islamic decorations, and after the theoretical study, the researcher prepared a teaching program cons-isting of 6 interviews to apply it to the students of the fifth year of the College of Art Education to arrive at the creation of a metal hanging based on the letters of the Thu-luth calligraphy, and the Islamic floral decorations, The researcher has reached many results, the most important of which is that the Thuluth calligraphy, is a rich and fertile source through the diversity of design methods between overlay, condensation, redu-ction, enlargement and merging, which gives diversity and contrast in artwork in gen-eral and in metalwork in particular, writings and letters are compatible with many formation methods. Metal craftsmanship, which makes it a fertile source for metal-working specialization and easy to teach, This is what was shown by the results of the experiment. The aim of the search is to Combining calligraphy and plant motifs as a springboard to achieve the vision of the plastic surfaces of metal busy with the students of the Faculty of Art Education

Just Gaze and Wave: Exploring the Use of Gaze and Gestures for Shoulder-surfing Resilient Authentication

Eye-gaze and mid-air gestures are promising for resisting various types of side-channel attacks during authentication. However, to date, a comparison of the different authentication modalities is missing. We investigate multiple authentication mechanisms that leverage gestures, eye gaze, and a multimodal combination of them and study their resilience to shoulder surfing. To this end, we report on our implementation of three schemes and results from usability and security evaluations where we also experimented with fixed and randomized layouts. We found that the gaze-based approach outperforms the other schemes in terms of input time, error rate, perceived workload, and resistance to observation attacks, and that randomizing the layout does not improve observation resistance enough to warrant the reduced usability. Our work further underlines the significance of replicating previous eye tracking studies using today's sensors as we show significant improvement over similar previously introduced gaze-based authentication systems

Hybrid MCDM Model of ARAS -TOPSIS - GRA for Materials Selection Problem

Since traditional Multi-Criteria Decision Making (MCDM) approaches have become defunct, academics have shifted their attention to developing hybrid MCDM models, which use a combination of two or more MCDM methods to solve decision-making issues. Using Additive Ratio Assessment (ARAS), the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS), and Gray Relational Analysis (GRA), the authors of this work aimed to develop unique hybrid MCDM systems. To show how well this hybrid model works, it is applied to a real-world material selection scenario including seven possible materials and seven criteria. Results from this hybrid model are compared to those from other standalone MCDM tools as well as previously published findings based on the same illustrative situation. 3rd Material is the best option, while the 2nd material is the worst option, among these seven choices which are consistent enough to imply such a conclusion. Using many MCDM strategies is recommended since no one strategy can ensure making the best decision. Because of this, the Copeland approach is used to combine the rankings from the 11 methodologies and get a consensus result. The results from the Copeland technique show that the final consensus rank of materials may be different from the rank of the hybrid model and other standalone MCDM tools. As a result, it is crucial to use a multi-pronged approach. Furthermore, the Spearman Correlation Coefficient (SCC) shows that the suggested rankings produced from the different approaches have a significant ranking association with one another

Association Between IL-10 Levels With Plasmodim Falciparum Related to Age Groups and Density of Infection Among Sudanese Patients- Khartoum State- Sudan

Abstract: A complex parasite such as human Plasmodium is likely to generate a variety of substances that injure the hosts directly or cause immunopathology. In malaria, a blood concentration of anti-inflammatory cytokines, such as interleukin (IL-10) is increased. The present study was performed to analyze IL-10 levels in patients with malaria falciparum and healthy controls individuals and correlate with malaria density infection as well as age groups. It is a cross sectional study was carried out in Khartoum state /Sudan, a total of 70 febrile patients and 20 healthy volunteers as controls were included in this study. Blood samples were collected from both patients and control candidates in (EDTA) containers for parasitological and immunological tests. Immunochromatography test and microscopic examination for both thick and thin blood films were used to detect the positive samples for Plasmodium falciparum. Enzyme linked Immunosorbant Assay (ELISA), was used for the determination of IL-10 levels. The result showed that of IL-10 levels in malaria patients were 1598.50 and SD 4771.64 while the levels in healthy participants the level was 10.39 and SD was 124.33 the difference was found to be statistically significant (P= 0.007). The levels of cytokines mentioned above were observed to be raised in malaria individual compared to healthy control candidates

Integration of SCADA, GIS, and Call Center Systems for Electrical Power Distribution Management and Planning

In electrical power distribution systems the traditional methods cannot detect the customer fault location in real time and respond to customer complaints at the same time of the outage of the electric power because the required information is scattered among isolated databases. In this paper the combination of Supervisory Control and Data Acquisition (SCADA) and Geographical Information System (GIS) and using SQLCMD against DBSET has been shown to solve this problem better. This paper reducing the response time of the customer waiting when they calling the agent in the call center, creating a model for the integration of real time data in SCADA system against static data in GIS to make online GIS and send data from GIS to CALL CENTER at the same time. The prototype describes the flow of the data between various systems and integrates all in one logical database that contains all data about the customers. The proposed model depends on three major sub-systems: GIS, SCADA, and Call Center systems. The GIS system is base of the model so the change and update in GIS database is available, GIS provides different features like maps, real coordinates and tables. The model contains three different databases, GIS as geo database, SCADA as the real time database and call center as customer information database, all this database will be in one logical global database that contains of spatial information tables, asset information tables, topology information tables, and operation information tables. This method has been shown to significantly improve the accuracy and efficiency of fault detection in distribution networks and to decrease the response time in call centers

Influence of Platelets Rich Plasma on Repair of Superficial Digital Flexor Tendon Rupture in Donkeys (An Experimental Study)

This study was planned to prospectively evaluate the influence of platelets rich plasma (PRP) on the restoration of superficial digital flexor tendon (SDFT) in donkeys. Eighteen apparently clinically healthy male donkeys of 3-6 years old, weighing 150-200 kg, were used. The animals were classified into two equal groups; control group and PRP group. One cm transection of the full thickness of middle portion of the SDFT was made in all animals. First, all animals were subjected to sedation via xylazine HCl injection in a dose of 1 mg/kg body weight then the animals were deeply narcotized with chloral hydrate 10% in a dose of 5 gm/50 kg body weight. A linear skin incision in the palmar aspect of the right fore limb at the mid cannon region was performed in all operated animals. The animals in PRP group were subjected to intratendinous injections of 4 mL PRP with weekly interval (day 0, w1, w2, w3) under the guidance of ultrasonography. Representative tendon specimens from all animals were collected 30, 60, and 90 days post operation (PO) for histopathological examination. The obtained clinical and histopathological results reveled that PRP application improved the healing of SDFT compared to the control group, through increasing and acceleration of angiogenesis, fibrogenesis, collagen deposition and remodeling in the injured tendon matrices

Apoptotic Inhibitors as Therapeutic Targets for Cell Survival

Apoptosis has revealed an essential function in the development or prevention of oncogenic transformation in the body; however, programmed cell death (PCD) must be tightly controlled since deregulated cell death is involved in the development of a large number of different pathologies. Apoptosis can be decreased in pathological states such as in cancer and autoimmunity or elevated such as in stroke, neurodegeneration, retinal cell death, myocardial and liver ischemia, inflammatory diseases such as sepsis, osteoarthritis (OA), rheumatoid arthritis (RA), and asthma. Different types of apoptotic inhibitors will be discussed in this chapter displaying their mechanism of action, which have been reported to be therapeutic targets for cell survival or at least limiting cell death. These inhibitors are classified according to their nature into natural antiapoptotic proteins that present mainly in the cell and synthetic small molecule inhibitors that are widely used to protect against overexpression of apoptosis mediators and, in turn, to prevent corresponding diseases

Review of Recommender Systems Algorithms Utilized in Social Networks based e-Learning Systems & Neutrosophic System

In this paper, we present a review of different recommender system algorithms that are utilized in social networks based e-Learning systems. Future research will include our proposed our e-Learning system that utilizes Recommender System and Social Network. Since the world is full of indeterminacy, the neutrosophics found their place into contemporary research. The fundamental concepts of neutrosophic set, introduced by Smarandache in [21, 22, 23] and Salama et al. in [24-66].The purpose of this paper is to utilize a neutrosophic set to analyze social networks data conducted through learning activities