Does loneliness lurk in temp work? Exploring the associations between temporary employment, loneliness at work and job satisfaction

This research contributes to the limited literature concerning the determinants of loneliness at work, as well as to the literature on psychological outcomes associated with temporary work. More specifically, we are adding to the literature by exploring whether there is an association between working temporarily and loneliness at work and whether loneliness at work partly explains the association between working temporarily and job satisfaction. To this end, we analyse—by means of a mediation model—a unique sample of Flemish employees in the private sector. We find that employees with a temporary contract experience more loneliness at work as opposed to employees with a permanent contract. In addition, we discover that loneliness at work mediates the association between working temporarily and job satisfaction

A dynamic dashboarding application for fleet monitoring using semantic web of things technologies

In industry, dashboards are often used to monitor fleets of assets, such as trains, machines or buildings. In such industrial fleets, the vast amount of sensors evolves continuously, new sensor data exchange protocols and data formats are introduced, new visualization types may need to be introduced and existing dashboard visualizations may need to be updated in terms of displayed sensors. These requirements motivate the development of dynamic dashboarding applications. These, as opposed to fixed-structure dashboard applications, allow users to create visualizations at will and do not have hard-coded sensor bindings. The state-of-the-art in dynamic dashboarding does not cope well with the frequent additions and removals of sensors that must be monitored—these changes must still be configured in the implementation or at runtime by a user. Also, the user is presented with an overload of sensors, aggregations and visualizations to select from, which may sometimes even lead to the creation of dashboard widgets that do not make sense. In this paper, we present a dynamic dashboard that overcomes these problems. Sensors, visualizations and aggregations can be discovered automatically, since they are provided as RESTful Web Things on a Web Thing Model compliant gateway. The gateway also provides semantic annotations of the Web Things, describing what their abilities are. A semantic reasoner can derive visualization suggestions, given the Thing annotations, logic rules and a custom dashboard ontology. The resulting dashboarding application automatically presents the available sensors, visualizations and aggregations that can be used, without requiring sensor configuration, and assists the user in building dashboards that make sense. This way, the user can concentrate on interpreting the sensor data and detecting and solving operational problems early

The mitotic checkpoint is a targetable vulnerability of carboplatin-resistant triple negative breast cancers

Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype, lacking effective therapy. Many TNBCs show remarkable response to carboplatin-based chemotherapy, but often develop resistance over time. With increasing use of carboplatin in the clinic, there is a pressing need to identify vulnerabilities of carboplatin-resistant tumors. In this study, we generated carboplatin-resistant TNBC MDA-MB-468 cell line and patient derived TNBC xenograft models. Mass spectrometry-based proteome profiling demonstrated that carboplatin resistance in TNBC is linked to drastic metabolism rewiring and upregulation of anti-oxidative response that supports cell replication by maintaining low levels of DNA damage in the presence of carboplatin. Carboplatin-resistant cells also exhibited dysregulation of the mitotic checkpoint. A kinome shRNA screen revealed that carboplatin-resistant cells are vulnerable to the depletion of the mitotic checkpoint regulators, whereas the checkpoint kinases CHEK1 and WEE1 are indispensable for the survival of carboplatin-resistant cells in the presence of carboplatin. We confirmed that pharmacological inhibition of CHEK1 by prexasertib in the presence of carboplatin is well tolerated by mice and suppresses the growth of carboplatin-resistant TNBC xenografts. Thus, abrogation of the mitotic checkpoint by CHEK1 inhibition re-sensitizes carboplatin-resistant TNBCs to carboplatin and represents a potential strategy for the treatment of carboplatin-resistant TNBCs

Assessing the drivers behind the structure and diversity of fish assemblages associated with rocky shores in the Galapagos archipelago

Oceanic islands harbor unique yet fragile marine ecosystems that require evidence-based environmental management. Among these islands, the Galapagos archipelago is well known for its fish diversity, but the factors that structure communities within and between its islands remain poorly understood. In this study, water quality, physical habitats and geographical distance were assessed as potential predictors for the diversity and structure of fish assemblages. Differences in the structure of fish assemblages of the two studied islands (Santa Cruz and Floreana) were most likely driven by temperature and nutrient concentrations. In the relatively highly populated island Santa Cruz, the structure of fish assemblages was more affected by water conditions than physical habitats while the contrary was true for the more pristine area of Floreana. A wide variety of species with different geographical origins were distributed over the different islands, which indicates that most fish species are able to reach the islands of the archipelago. However, temperature gradients and elevated nutrient levels cause large differences in the structure of local fish assemblages. In addition, in Santa Cruz nutrient concentrations were negatively correlated with α diversity. Since pollution is a clear pressure on the fish assemblages of oceanic islands, environmental management of the coastal areas is of paramount importance

Emigration of European silver eel (Anguilla anguilla L.) from a polder system into the Schelde estuary

Connectivity between freshwater habitats and marine areas is heavily obstructed by migration barriers, leading to a high pressure on diadromous eel populations. Migration barriers attribute to the 98% decline of the European eel (Anguilla anguilla L.) population. A better understanding of eel behaviour regarding these barriers is needed for water managers to take proper mitigation actions. We tracked 50 eels by means of acoustic telemetry between July 2012 and January 2015 and analysed their migration behaviour in a Belgian polder area. On their way to the Schelde Estuary, eels face several migration barriers such as a pumping station, a weir and tidal barriers. The telemetry study did reveal significant delays and searching behaviour near those barriers. Depending on nothing but their accumulated fat, delays can have a serious impact on the fitness of the eel by wasting precious energy resources needed for a successful trans-Atlantic migration. In addition, delays and searching behaviour can also increase predation risk. The obtained knowledge can contribute to efficient management such as improved fish passage and guidance solutions

Plasma cells are not restricted to the CD27+ phenotype:characterization of CD27-CD43+ antibody-secreting cells

Circulating antibody-secreting cells are present in the peripheral blood of healthy individuals reflecting the continued activity of the humoral immune system. Antibody-secreting cells typically express CD27. Here we describe and characterize a small population of antibody-secreting class switched CD19+CD43+ B cells that lack expression of CD27 in the peripheral blood of healthy subjects. In this study, we characterized CD27-CD43+ cells. We demonstrate that class-switched CD27-CD43+ B cells possess characteristics of conventional plasmablasts as they spontaneously secrete antibodies, are morphologically similar to antibody-secreting cells, show downregulation of B cell differentiation markers, and have a gene expression profile related to conventional plasmablasts. Despite these similarities, we observed differences in IgA and IgG subclass distribution, expression of homing markers, replication history, frequency of somatic hypermutation, immunoglobulin repertoire, gene expression related to Toll-like receptors, cytokines, and cytokine receptors, and antibody response to vaccination. Their frequency is altered in immune-mediated disorders. Conclusion: we characterized CD27-CD43+ cells as antibody-secreting cells with differences in function and homing potential as compared to conventional CD27+ antibody-secreting cells.</p