104 research outputs found
Rescaling Relations between Two- and Three-dimensional Local Porosity Distributions for Natural and Artificial Porous Media
Local porosity distributions for a three-dimensional porous medium and local
porosity distributions for a two-dimensional plane-section through the medium
are generally different. However, for homogeneous and isotropic media having
finite correlation-lengths, a good degree of correspondence between the two
sets of local porosity distributions can be obtained by rescaling lengths, and
the mapping associating corresponding distributions can be found from
two-dimensional observations alone. The agreement between associated
distributions is good as long as the linear extent of the measurement cells
involved is somewhat larger than the correlation length, and it improves as the
linear extent increases. A simple application of the central limit theorem
shows that there must be a correspondence in the limit of very large
measurement cells, because the distributions from both sets approach normal
distributions. A normal distribution has two independent parameters: the mean
and the variance. If the sample is large enough, LPDs from both sets will have
the same mean. Therefore corresponding distributions are found by matching
variances of two- and three-dimensional local porosity distributions. The
variance can be independently determined from correlation functions. Equating
variances leads to a scaling relation for lengths in this limit. Three
particular systems are examined in order to show that this scaling behavior
persists at smaller length-scales.Comment: 15 PostScript figures, LaTeX, To be published in Physica
A three-tiered study of differences in murine intrahost immune response to multiple pneumococcal strains
We apply a previously developed 4-variable ordinary differential equation model of in-host immune response to pneumococcal pneumonia to study the variability of the immune response of MF1 mice and to explore bacteria-driven differences in disease progression and outcome. In particular, we study the immune response to D39 strain of bacteria missing portions of the pneumolysin protein controlling either the hemolytic activity or complement-activating activity, the response to D39 bacteria deficient in either neuraminidase A or B, and the differences in the response to D39 (serotype 2), 0100993 (serotype 3), and TIGR4 (serotype 4) bacteria. The model accurately reproduces infection kinetics in all cases and provides information about which mechanisms in the immune response have the greatest effect in each case. Results suggest that differences in the ability of bacteria to defeat immune response are primarily due to the ability of the bacteria to elude nonspecific clearance in the lung tissue as well as the ability to create damage to the lung epithelium
Predictability of Self-Organizing Systems
We study the predictability of large events in self-organizing systems. We
focus on a set of models which have been studied as analogs of earthquake
faults and fault systems, and apply methods based on techniques which are of
current interest in seismology. In all cases we find detectable correlations
between precursory smaller events and the large events we aim to forecast. We
compare predictions based on different patterns of precursory events and find
that for all of the models a new precursor based on the spatial distribution of
activity outperforms more traditional measures based on temporal variations in
the local activity.Comment: 15 pages, plain.tex with special macros included, 4 figure
Reflectivity Anisotropy Spectra of Cu- and Ag- (110) surfaces from {\it ab initio} theory
We are able to disentagle the effects of the intraband and interband parts of
the bulk dielectric function on the bare dielectric anisotropy of the surface.
We show how the position, sign and amplitude of the structures observed in such
spectra depend on the above quantities. The lineshape of all the calculated
structures agree very well with the ones observed experimentally for samples
treated by suitable surface cleaning. In particular, we reproduce the observed
single peak structure of Ag at high energy, found to represent a state of the
clean surface different from the one giving the originally observed double peak
structure. This results is not reproduced by the 'local field' model.Comment: 4 pages, 3 figures. submitted to Phys. Rev. Let
Discrete structure of ultrathin dielectric films and their surface optical properties
The boundary problem of linear classical optics about the interaction of
electromagnetic radiation with a thin dielectric film has been solved under
explicit consideration of its discrete structure. The main attention has been
paid to the investigation of the near-zone optical response of dielectrics. The
laws of reflection and refraction for discrete structures in the case of a
regular atomic distribution are studied and the structure of evanescent
harmonics induced by an external plane wave near the surface is investigated in
details. It is shown by means of analytical and numerical calculations that due
to the existence of the evanescent harmonics the laws of reflection and
refraction at the distances from the surface less than two interatomic
distances are principally different from the Fresnel laws. From the practical
point of view the results of this work might be useful for the near-field
optical microscopy of ultrahigh resolution.Comment: 25 pages, 16 figures, LaTeX2.09, to be published in Phys.Rev.
Effect of physical activity, social support, and skills training on late-life emotional health: a systematic literature review and implications for public health research
Purpose: Given that emotional health is a critical component of healthy aging, we undertook a systematic literature review to assess whether current interventions can positively affect older adults’ emotional health.
Methods: A national panel of health services and mental health researchers guided the review. Eligibility criteria included community-dwelling older adult (aged ≥ 50 years) samples, reproducible interventions, and emotional health outcomes, which included multiple domains and both positive (well-being) and illness-related (anxiety) dimensions. This review focused on three types of interventions – physical activity, social support, and skills training – given their public health significance and large number of studies identified. Panel members evaluated the strength of evidence (quality and effectiveness).
Results: In all, 292 articles met inclusion criteria. These included 83 exercise/physical activity, 25 social support, and 40 skills training interventions. For evidence rating, these 148 interventions were categorized into 64 pairings by intervention type and emotional health outcome, e.g., strength training targeting loneliness or social support to address mood. 83% of these pairings were rated at least fair quality. Expert panelists found sufficient evidence of effectiveness only for skills training interventions with health outcomes of decreasing anxiety and improving quality of life and self-efficacy. Due to limitations in reviewed studies, many intervention–outcome pairings yielded insufficient evidence.
Conclusion: Skills training interventions improved several aspects of emotional health in community-dwelling older adults, while the effects for other outcomes and interventions lacked clear evidence. We discuss the implications and challenges in moving forward in this important area
Mediator of DNA Damage Checkpoint 1 (MDC1) Contributes to High NaCl-Induced Activation of the Osmoprotective Transcription Factor TonEBP/OREBP
Background: Hypertonicity, such as induced by high NaCl, increases the activity of the transcription factor TonEBP/OREBP whose target genes increase osmoprotective organic osmolytes and heat shock proteins. Methodology: We used mass spectrometry to analyze proteins that coimmunoprecipitate with TonEBP/OREBP in order to identify ones that might contribute to its high NaCl-induced activation. Principal Findings: We identified 20 unique peptides from Mediator of DNA Damage Checkpoint 1 (MDC1) with high probability. The identification was confirmed by Western analysis. We used small interfering RNA knockdown of MDC1 to characterize its osmotic function. Knocking down MDC1 reduces high NaCl-induced increases in TonEBP/OREBP transcriptional and transactivating activity, but has no significant effect on its nuclear localization. We confirm six previously known phosphorylation sites in MDC1, but do not find evidence that high NaCl increases phosphorylation of MDC1. It is suggestive that MDC1 acts as a DNA damage response protein since hypertonicity reversibly increases DNA breaks, and other DNA damage response proteins, like ATM, also associate with TonEBP/OREBP and contribute to its activation by hypertonicity. Conclusions/Significance: MDC1 associates with TonEBP/OREBP and contributes to high NaCl-induced increase of tha
Mathematical models for immunology:current state of the art and future research directions
The advances in genetics and biochemistry that have taken place over the last 10Â years led to significant advances in experimental and clinical immunology. In turn, this has led to the development of new mathematical models to investigate qualitatively and quantitatively various open questions in immunology. In this study we present a review of some research areas in mathematical immunology that evolved over the last 10Â years. To this end, we take a step-by-step approach in discussing a range of models derived to study the dynamics of both the innate and immune responses at the molecular, cellular and tissue scales. To emphasise the use of mathematics in modelling in this area, we also review some of the mathematical tools used to investigate these models. Finally, we discuss some future trends in both experimental immunology and mathematical immunology for the upcoming years
Proteomic characterization of HIV-modulated membrane receptors, kinases and signaling proteins involved in novel angiogenic pathways
<p>Abstract</p> <p>Background</p> <p>Kaposi's sarcoma (KS), hemangioma, and other angioproliferative diseases are highly prevalent in HIV-infected individuals. While KS is etiologically linked to the human herpesvirus-8 (HHV8) infection, HIV-patients without HHV-8 and those infected with unrelated viruses also develop angiopathies. Further, HIV-Tat can activate protein-tyrosine-kinase (PTK-activity) of the vascular endothelial growth factor receptor involved in stimulating angiogenic processes. However, Tat by itself or HHV8-genes alone cannot induce angiogenesis <it>in vivo </it>unless specific proteins/enzymes are produced synchronously by different cell-types. We therefore tested a hypothesis that <it>chronic </it>HIV-<it>replication in non-endothelial cells </it>may produce novel factors that provoke angiogenic pathways.</p> <p>Methods</p> <p>Genome-wide proteins from HIV-infected and uninfected T-lymphocytes were tested by subtractive proteomics analyses at various stages of virus and cell growth <it>in vitro </it>over a period of two years. Several thousand differentially regulated proteins were identified by mass spectrometry (MS) and >200 proteins were confirmed in multiple gels. Each protein was scrutinized extensively by protein-interaction-pathways, bioinformatics, and statistical analyses.</p> <p>Results</p> <p>By functional categorization, 31 proteins were identified to be associated with various signaling events involved in angiogenesis. 88% proteins were located in the plasma membrane or extracellular matrix and >90% were found to be essential for regeneration, neovascularization and angiogenic processes during embryonic development.</p> <p>Conclusion</p> <p>Chronic HIV-infection of T-cells produces membrane receptor-PTKs, serine-threonine kinases, growth factors, adhesion molecules and many diffusible signaling proteins that have not been previously reported in HIV-infected cells. Each protein has been associated with endothelial cell-growth, morphogenesis, sprouting, microvessel-formation and other biological processes involved in angiogenesis (p = 10<sup>-4 </sup>to 10<sup>-12</sup>). Bioinformatics analyses suggest that overproduction of PTKs and other kinases in HIV-infected cells has <it>suppressed </it>VEGF/VEGFR-PTK expression and promoted <it>VEGFR-independent </it>pathways. This unique mechanism is similar to that observed in neovascularization and angiogenesis during embryogenesis. Validation of clinically relevant proteins by gene-silencing and translational studies <it>in vivo </it>would identify specific targets that can be used for early diagnosis of angiogenic disorders and future development of inhibitors of angiopathies. This is the first comprehensive study to demonstrate that HIV-infection alone, without any co-infection or treatment, can induce numerous "embryonic" proteins and kinases capable of generating novel <it>VEGF-independent </it>angiogenic pathways.</p
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