11 research outputs found
Additional file 2: of Association between different anti-Tat antibody isotypes and HIV disease progression: data from an African cohort
magnitude of anti-Tat humoral responses: (A) Anti-clade B and C Tat IgG, IgA or IgM titers were determined in anti-Tat positive sera and displayed for every donor. (B) Heatmap showing, for single donors, positivity (black) or negativity (grey) towards clade C or clade B Tat for each isotype. (C) Optical density (O.D.) from screening ELISA tests are shown for each donor after cut-off subtraction. Cut-off values were included in the following ranges: 0.128 ± 0.029 for anti-clade C Tat IgG; 0.1245 ± 0.042 for anti-clade B Tat IgG; 0.1177 ± 0.022 for anti-clade C Tat IgA; 0.1387 ± 0.031 for anti-clade C Tat IgA; 0.1963 ± 0.060 for anti-clade C Tat IgM; 0.370 ± 0.086 for anti-clade B Tat IgM. (JPG 154 kb
Expression of systemic T cell activation markers in relation to infection with different helminth species.
<p>The frequencies of HLA-DR<sup>+</sup>CD38<sup>−</sup> and total HLA-DR<sup>+</sup> (B) are shown on the y-axis for CD4 (left panels) and CD8 T cells (right panels). The worm infection status is indicated on the x-axis stratified into worm negative individuals or those infected with TT (<i>Trichuris trichiura</i>), SH (<i>Schistosoma haematobium</i>), SM (<i>Schistosoma mansoni</i>), AL (<i>Ascaris lumbricoides</i>) or HW (Hookworm). Statistical analysis was performed using Mann-Whitney test for comparing groups. Shown in (C) is a linear regression analysis between the frequency of HLA-DR<sup>+</sup>/CD38<sup>+</sup> CD8 T cells and the worm egg counts (as measured by Kato-Katz method) within Trichuris (left panel) and <i>S. mansoni</i> (right panel) infected subjects.</p
Additional file 3: of Association between different anti-Tat antibody isotypes and HIV disease progression: data from an African cohort
Frequencies of CD4+ and CD8+ T cells expressing activation and maturation markers in the peripheral blood: Shown are representative plots demonstrating the gating strategy for the expression of activation (CD38 and HLA-DR; left panel) and maturation (CD27 and CD45RO; right panel) markers on CD4+ (upper panels) and CD8+ (lower panels) T cells. (JPG 316 kb
Expression of activation markers on CD4 and CD8 T cells in relation to chronic infection with different helminth species on HIV negative individuals.
<p>*P values for comparison between helminth infected and non-infected controls were calculated using the Mann-Whitney test.</p
CD27 and Mip-1β expression of CMV specific IFNγ producing CD4 T cell responses are influenced by HIV as well as tuberculosis co-infection in adults.
<p>Scatter-plots illustrating (A) the CD27 MFI fold-change to all CD4<sup>+</sup> T cells and B) the proportion of cells producing Mip-1β of CMV specific IFNγ<sup>+</sup>-CD4<sup>+</sup> T cell in patients under 10 or over 18 years old and according to HIV and TB infection status.</p
CD27 and Mip-1β expression of CMV specific IFNγ producing CD8 T cell responses are not significantly influenced by HIV as well as tuberculosis co-infection in adults.
<p>Scatter-plots illustrating (A) the CD27 MFI fold-change to all CD8<sup>+</sup> T cells and B) the proportion of cells producing Mip-1β of CMV specific IFNγ<sup>+</sup>-CD8<sup>+</sup> T cell in patients under 10 or over 18 years old and according to HIV and TB infection status.</p
Frequencies and absolute counts of IFNγ<sup>+</sup>-CMVpp65–specific CD4<sup>+</sup> or CD8<sup>+</sup> T cell in children below 10 years of age and adults.
<p>Scatter-plots depicting A) the frequencies or absolute counts of CMV specific IFNγ<sup>+</sup>-CD4<sup>+</sup> T cells s and C) frequencies of CMV specific IFNγ<sup>+</sup>-CD8<sup>+</sup> T cells in the two-age groups and according to HIV status.</p
Gating strategies and CMV responses evaluation.
<p>A) Morphological and CD marker expression gating strategy of CD4 and CD8 T cell. B) Representative CD4 and CD8 T cell IFNγ response from a significant responder. C) Gating strategy for the CD27 MFI Fold Change calculation of IFNγ producing cells compared to all CD4 or CD8 T cells. D) Gating strategy for the determination of IFNγ producing cells that were also positive for Mip1β production.</p
Frequencies and absolute counts of IFNγ<sup>+</sup>-SEB–specific CD4<sup>+</sup> or CD8<sup>+</sup> T cell in children below 10 years of age and adults.
<p>Scatter-plots depicting A) the frequencies or B) absolute counts of SEB specific IFNγ<sup>+</sup>-CD4<sup>+</sup> T cells s and C) frequencies of SEB specific IFNγ<sup>+</sup>-CD8<sup>+</sup> T cells in the two-age groups and according to HIV status.</p