magnitude of anti-Tat humoral responses: (A) Anti-clade B and C Tat IgG, IgA or IgM titers were determined in anti-Tat positive sera and displayed for every donor. (B) Heatmap showing, for single donors, positivity (black) or negativity (grey) towards clade C or clade B Tat for each isotype. (C) Optical density (O.D.) from screening ELISA tests are shown for each donor after cut-off subtraction. Cut-off values were included in the following ranges: 0.128 ± 0.029 for anti-clade C Tat IgG; 0.1245 ± 0.042 for anti-clade B Tat IgG; 0.1177 ± 0.022 for anti-clade C Tat IgA; 0.1387 ± 0.031 for anti-clade C Tat IgA; 0.1963 ± 0.060 for anti-clade C Tat IgM; 0.370 ± 0.086 for anti-clade B Tat IgM. (JPG 154 kb
