Literature review of research trends on unnatural deaths in older adult in Japan from 2000 to 2020: A text mining analysis

The purpose of this study was to determine the current state of research on unnatural deaths　in older adults in Japan. We searched original articles published in Japan from 2000 to 2020 by using the key terms“ older adult” and“ unnatural death” and identified 32 articles. We analyzed the articles by quantitative text mining with KH Coder （ver.3.0） software. The most frequently extracted words were“ death,”“ many,”“ discovery,”“ suicide,”“ cause of death,” and“ autopsy.” In the co-occurrence network analysis of words,“ death,”“ many,”“ suicide,” and“ man” had high mediation centrality. We used subgraph detection to classify the top 50 words into 5 groups: “Time delay before discovery of single older adult men after death,”“ Trend for unnatural deaths of missing older adults,”“ Relationship between mental illness and solitary death,”“ Investigation of cause of death by forensic autopsy,” and “Measures for preventing solitary death of older adults isolated from society.” Currently, studies are limited regarding possible measures to prevent solitary deaths among older adults isolated from society. In Japan, there is an urgent need to support older adults at risk of social isolation, such as single men and people with mental illnesses, to help maintain or improve their social integration

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target

X-ray Radiography Inspection of Pores of Thin Aluminum Foam during Press Forming Immediately after Foaming

Forming aluminum foam to the desired shape while retaining its pore structures is essential for manufacturing aluminum foam products. Recently, a press forming process for aluminum foam that is performed after precursor foaming but before solidification has been proposed. In this study, to track individual pores throughout press forming immediately after foaming, X-ray radiography inspection was applied. A thin precursor was used, and foaming was constrained to the X-ray transmission direction. It was shown that, although some pores coalesced with other pores, the pores did not collapse during press forming. In addition, the porosity of aluminum foam evaluated from X-ray transmission images was constant during press forming. Some pores retained their shape during press forming but their position was changed by the material flow generated by press forming. These results show that by press forming before the solidification of aluminum foam, aluminum foam can be shaped without the collapse of pores

Comparison of environmental intolerances and symptoms between patients with multiple chemical sensitivity, subjects with self-reported electromagnetic hypersensitivity, patients with bronchial asthma, and the general population

Abstract Background Environmental hypersensitivity/intolerance is considered closely related to allergic diseases. To understand these conditions, the environmental intolerances and symptoms of patients with multiple chemical sensitivity (MCS), subjects with self-reported electromagnetic hypersensitivity (EHS), patients with bronchial asthma (BA), and the general population were compared using universal questionnaires. Methods A survey was conducted from 2012 to 2015. The subjects were categorized in four groups: 111 patients with physician-diagnosed MCS, 119 subjects with self-reported EHS, patients with 98 physician-diagnosed BA, and 619 controls from general population. The Quick Environmental Exposure and Sensitivity Inventory and EHS questionnaire were used. The differences between the questionnaire scores among the four groups were tested using logistic regression analyses adjusted for age and gender as covariates. Results The MCS and EHS groups had significantly high scores of intolerances to multiple environmental factors, life impact, and multiple symptoms than the BA and control groups. Although the differences between most of these scores of the MCS and EHS groups were not significant, the electromagnetic hypersensitivity reaction was significantly higher in the EHS group than in the MCS group. In addition, the scores for intolerances to chemicals and other compounds, life impact, and several symptoms of the BA group were significantly higher than those of the control group. Conclusions This study clarified the similarities and differences of the environmental intolerances and symptoms between the four groups