Identification Of Anln As Etv6 Partner Gene In Recurrent T(7;12)(p15;p13): A Possible Role Of Deregulated Anln Expression In Leukemogenesis.

The ETV6 gene encodes an ETS family transcription factor that is involved in a myriad of chromosomal rearrangements found in hematological malignancies and other neoplasms. A recurrent ETV6 translocation, previously described in patients with acute myeloid leukemia (AML) (Genes Chromosomes Cancer 51:328-337,2012, Leuk Res 35:e212-214, 2011), whose partner has not been identified is t(7;12)(p15;p13). We herein report that the t(7;12)(p15;p13) fuses ETV6 to ANLN, a gene not previously implicated in the pathogenesis of hematological malignancies, and we demonstrate that this translocation leads to high expression of the fusion transcript in the myeloid and lymphoid lineages.1419

Aerobic exercise minimizes cisplatin-induced cachexia and renal damage.

<p>Exercise efficacy was confirmed by an increase in muscle angiogenesis in the exercised (EX) and exercised with cisplatin injection (CIS-EX) groups (<b>A</b> and <b>B</b>). Decreased weight loss (<b>C</b>) and lower levels of serum urea (<b>D</b>) in the CIS-EX group compared to the sedentary goup injected with cisplatin (CIS) indicate the protective effect of exercise. The sedentary group without cisplatin injection was used as the control (CT). *P<0.05; **P<0.01; ***P<0.001.</p

Aerobic exercise reduces cell death in the kidney.

<p>Representative TUNEL staining (<b>A</b>) and quantification (<b>B</b>) showed a significantly reduced percentage of TUNEL-positive cells (dead cells) in the CIS-EX group. The expression of Bcl2 and Bax genes was evaluated by qPCR and is represented by the ratio of the pro- to the anti-apoptotic molecule (Bax/Bcl2) (<b>C</b>) the CIS group was more likely to exhibit a pro-apoptotic profile, confirming the protective role of exercise. ***P<0.001.</p

IL-6 contributes to kidney protection induced by exercise.

<p>The expression of the cytokine IL-6 was determined by qPCR in the muscle (<b>A</b>) and the kidney (<b>B</b>). The CIS group showed increased levels of IL-6 in both tissues compared to sedentary groups (CT and EX). However, CIS-EX showed increased IL-6 expression in the kidney, which indicates the protective role of IL-6 in this model. *P<0.05; **P<0.01.</p

Chronic training.

<p>*Cisplatin administration.</p><p>Chronic training.</p

Exercise promotes increased HO-1 expression.

<p>Nrf2 and HO-1 expression in the kidney were evaluated by Western Blot (<b>left</b> – representative image and <b>right</b> – quantification). Exercise induced HO-1 gene expression, as confirmed by qPCR (<b>B</b>), and the levels of HO-1 in the renal tissue were analyzed by immunohistochemistry (<b>C</b>, <b>left</b> – representative image and <b>right</b> – quantification). *P<0.05; **P<0.01; ***P<0.001.</p

Exercise attenuates inflammation.

<p>qPCR analysis revealed that the CIS group showed higher expressions of TNF (<b>A</b>) and IL-10 (<b>B</b>) in the kidney, while CIS-EX did not exhibit significant differences compared to CT. The serum concentrations of TNF (<b>C</b>) and IL-10 (<b>D</b>), determined by CBA, appeared to decrease in the CIS-EX group. *P<0.05; **P<0.01; ***P<0.001; N.D. - non detected cytokine.</p