Characterization of Mesenchymal Stem Cells in Musculoskeletal Injury

The main goal of regenerative medicine is to enhance the innate healing response to more closely mimic tissue development. This may include cell-based, trophic, or small molecule therapies. In cell-based therapies, the initial premise was one of tissue replacement. Mesenchymal stem cells (MSCs) are adult-derived stem cells present throughout the body and easily harvested from individual patients. After over a decade of veterinary and human medical use of MSCs and medical research, the function of MSCs after therapeutic use in injury remains unclear. Elucidating engraftment location and longevity of MSCs post injection may provide insight to their mechanism of action. We developed a protocol for labeling MSCs with a fluorocarbon nanoparticle that allows for non-invasive longitudinal tracking and evaluated cellular viability, proliferation, and morphology. A dose dependent cell association of the nanoparticle was seen in our study but it was not repeatable between individuals. Prior to use of this technique to track MSCs, further study is needed to elucidate where the failure occurred: uptake of label by MSCs, maintenance of label within the cell cytoplasm or loss of conjugation of the fluorophore. Despite the use of MSCs as a therapeutic in horses for many years, there is little information on the best techniques for cryopreserving these cells for immediate use post thaw. We tested several freezing mediums for the short-term cryopreservation of equine MSCs. We found that 95% autologous serum and 5% DMSO did not negatively affect post thaw viability, growth kinetics, or morphology. Bisphosphonates were approved for use in horses in the United States in 2014. Since, they have become wildly popular due to improvements in lameness in treated horses. We suspected that the efficacy in lameness reduction could be due to an off target effect such as an effect on the MSC because the approved dose is so low compared to the anti- resorptive dose of the same drugs in people. We investigated the impact of bisphosphonates on bone remodeling and bone cells including MSCs. We observed reduction in lameness but no changes in bone turnover or MSC characteristics

Characterization of Mesenchymal Stem Cells in Musculoskeletal Injury

The main goal of regenerative medicine is to enhance the innate healing response to more closely mimic tissue development. This may include cell-based, trophic, or small molecule therapies. In cell-based therapies, the initial premise was one of tissue replacement. Mesenchymal stem cells (MSCs) are adult-derived stem cells present throughout the body and easily harvested from individual patients. After over a decade of veterinary and human medical use of MSCs and medical research, the function of MSCs after therapeutic use in injury remains unclear. Elucidating engraftment location and longevity of MSCs post injection may provide insight to their mechanism of action. We developed a protocol for labeling MSCs with a fluorocarbon nanoparticle that allows for non-invasive longitudinal tracking and evaluated cellular viability, proliferation, and morphology. A dose dependent cell association of the nanoparticle was seen in our study but it was not repeatable between individuals. Prior to use of this technique to track MSCs, further study is needed to elucidate where the failure occurred: uptake of label by MSCs, maintenance of label within the cell cytoplasm or loss of conjugation of the fluorophore. Despite the use of MSCs as a therapeutic in horses for many years, there is little information on the best techniques for cryopreserving these cells for immediate use post thaw. We tested several freezing mediums for the short-term cryopreservation of equine MSCs. We found that 95% autologous serum and 5% DMSO did not negatively affect post thaw viability, growth kinetics, or morphology. Bisphosphonates were approved for use in horses in the United States in 2014. Since, they have become wildly popular due to improvements in lameness in treated horses. We suspected that the efficacy in lameness reduction could be due to an off target effect such as an effect on the MSC because the approved dose is so low compared to the anti- resorptive dose of the same drugs in people. We investigated the impact of bisphosphonates on bone remodeling and bone cells including MSCs. We observed reduction in lameness but no changes in bone turnover or MSC characteristics

Disciplinary knowledge for what ends? The values dimension of curriculum research in the Anthropocene

This article makes the case for repositioning values and ethics as central to understanding how curriculum knowledge can be educationally powerful. Disciplinary knowledge can help individuals make sense of the present, explore alternative futures and participate in society, making ethical choices about how to live. This, however, depends on particular relationships between curriculum, disciplinary knowledge, values and ethical perspectives. We argue that the recent research agenda exploring disciplinary knowledge underplays the values dimension in how curriculum knowledge is constructed and used. First, we give an overview of the recent thrust of curriculum debates in subject education communities, placing this in some historical context. Here, we recognise the need to make a robust case for school subjects and their important relationship with disciplines. We go on to examine some arguments around the role of knowledge in curriculum. Taking the concept of the Anthropocene (the human epoch of the planet) and from our perspectives as geography and religious education educators, we propose a focus on ethical disposition and interdisciplinarity to make the values dimensions of curriculum knowledge more visible

THE EFFECTS OF INJURY AND DISPLACEMENT ON TYPHOON YOLANDA SURVIVORS

This study was conducted with a group Filipino survivors of Typhoon Yolanda. It examines the effects of being injured or displaced as a result of the natural disaster on the effectiveness of a pilot project addressing depression, anxiety and hope. Eight adults (N = 8) completed a series of five workshops along with a pre and post questionnaire. Those that were displaced, exhibited a reduction in depression (24%), a reduction in anxiety (12.8%), and an increase in hope (14.78%) after completing the workshops. Furthermore, the individuals that were injured experienced a reduction in anxiety (22.7%), an increase in depression (28.48%), and an increase in hope (14.86%)

Where the Trees Stood in Water

oai:currents.journals.yorku.ca:article/37295Where the Trees Stood in Water is a series of Cyanotype prints tracing the historic and contemporary transformations of Toronto’s Entertainment District. Each print is accompanied by an archival document which forms a narrative connecting geography to stories of colonization, industrialization and the transient bodies of those affected by the remaking of Toronto’s landscape. Juxtaposing text and image, Where the Trees… pushes against the archival documentation of Toronto’s past and challenges the stories told about colonialism, labour and migration. With playful reproductions, Bambitchell invites hidden, obscured, lost, and silenced narratives of colonial history to surface and tell new stories about the geography of Toronto’s core

Diseño de un colegio técnico industrial secundario, Chimbote 2020

La presente investigación, tuvo como objetivo general, elaborar el diseño de una escuela secundaria técnica industrial incorporando espacios comunes como elemento organizador, con el propósito de mejorar las condiciones de infraestructura educativa técnica secundaria en el distrito de Chimbote, otorgado así a la población adolescente, insertarse en el campo laboral. ya que la problemática principal en la ciudad, es que existe insuficientes centros educativos con esta tipologia,viendose así afectados los estudiantes en su crecimiento en actividades productivas tecnológicas y laborales, la metodología empleada, es de tipología descriptiva , con un diseño no experimental, con una orientación al análisis e interpretación de datos obtenidos en campo, provenientes de la escuela secundaria técnica “Politécnico”, para dicha la recolección se necesitó diseñar instrumentos, que fueron: fichas de observación, así como el cuestionario aplicado a los usuarios, las guías de entrevistas a los especialistas en el tema de estudio, etc.Finalmente,el resultado obtenido en la investigación, fue la elaboración de dicha propuesta arquitectónica y como satisfactoriamente funcionan ambas variables

Barriers and facilitators to accessing and utilising post-treatment psychosocial support by Black men treated for prostate cancer—a systematic review and qualitative synthesis

Purpose: To synthesise findings from published studies on barriers and facilitators to Black men accessing and utilising post-treatment psychosocial support after prostate cancer (CaP) treatment. Methods: Searches of Medline, Embase, PsycInfo, Cochrane Database of Systematic Reviews and Central, CINAHL plus and Scopus were undertaken from inception to May 2021. English language studies involving Black men aged ≥18 and reporting experiences of, or suggestions for, psychosocial support after CaP treatment were included. Low or moderate quality studies were excluded. Searches identified 4,453 articles and following deduplication, 2,325 were screened for eligibility. Two independent reviewers carried out screening, quality appraisal and data extraction. Data were analysed using thematic synthesis. Results: Ten qualitative studies involving 139 Black men were included. Data analysis identified four analytical constructs: experience of psychosocial support for dealing with treatment side effects (including impact on self-esteem and fear of recurrence); barriers to use of psychosocial support (such as perceptions of masculinity and stigma around sexual dysfunction); facilitators to use of psychosocial support (including the influence of others and self-motivation); and practical solutions for designing and delivering post-treatment psychosocial support (the need for trusted healthcare and cultural channels). Conclusions: Few intervention studies have focused on behaviours among Black CaP survivors, with existing research predominantly involving Caucasian men. There is a need for a collaborative approach to CaP care that recognises not only medical expertise but also the autonomy of Black men as experts of their illness experience, and the influence of cultural and social networks

Fluoride Adsorption Utilizing Magnetite Impregnated Bone Char

Reduce fluoride concentrations in contaminated mine water from 10 mg/L to below 2 mg/L. Design an energy efficient and sustainable system with a minimal carbon footprint. Recover Fluoride as a saleable produc