Detrimental and Protective Bystander Effects: A Model Approach

This work integrates two important cellular responses to low doses, detrimental bystander effects and apoptosis-mediated protective bystander effects, into a multistage model for chromosome aberrations and in vitro neoplastic transformation: the State-Vector Model. The new models were tested on representative data sets that show supralinear or U-shaped dose responses. The original model without the new low-dose features was also tested for consistency with LNT-shaped dose responses. Reductions of in vitro neoplastic transformation frequencies below the spontaneous level have been reported after exposure of cells to low doses of low-LET radiation. In the current study, this protective effect is explained with by-stander-induced apoptosis. An important data set that shows a low-dose detrimental bystander effect for chromosome aberrations was successfully fitted by additional terms within the cell initiation stage. It was found that this approach is equivalent to bystander-induced clonal expansion of initiated cells. This study is an important step toward a comprehensive model that contains all essential biological mechanisms that can influence dose–response curves at low doses

Radiation Induced Bystander Effects in Mice Given Low Doses of Radiation in Vivo

The ‘bystander effect’ phenomenon has challenged the traditional framework for assessing radiation damage by showing radiation induced changes in cells which have not been directly targeted, but are neighbors to or receive medium from directly hit cells. Our group performed a range of single and serial low dose irradiations on two genetically distinct strains of mice. Bladder explants established from these mice were incubated in culture medium, which was used to measure death responses in a keratinocyte reporter system. The study revealed that the medium harvested from bladder tissues’ (ITCM) from acutely irradiated C57BL6 but not Balb/c mice, was able to induce clonogenic death. Administration of a priming dose(s) before a challenge dose to both C57BL6 and Balb/c mice stimulated reporter cell survival irrespective of the time interval between dose(s) delivery. When ITCM corresponding to both strains of mice was measured for its calcium mobilization inducing ability, results showed an elevation in intracellular calcium levels that was strain dependent. This indicates that genotype determined the type of bystander signal/response that was produced after exposure to low and acute doses of radiation. However, serial exposure conditions modified bystander signal production to induce similar effects that were characterized by excessive growth

Human Lung Cancer Risks from Radon – Part I - Influence from Bystander Effects - A Microdose Analysis

Since the publication of the BEIR VI report in 1999 on health risks from radon, a significant amount of new data has been published showing various mechanisms that may affect the ultimate assessment of radon as a carcinogen, at low domestic and workplace radon levels, in particular the Bystander Effect (BE) and the Adaptive Response radio-protection (AR). We analyzed the microbeam and broadbeam alpha particle data of Miller et al. (1995, 1999), Zhou et al. (2001, 2003, 2004), Nagasawa and Little (1999, 2002), Hei et al. (1999), Sawant et al. (2001a) and found that the shape of the cellular response to alphas is relatively independent of cell species and LET of the alphas. The same alpha particle traversal dose response behavior should be true for human lung tissue exposure to radon progeny alpha particles. In the Bystander Damage Region of the alpha particle response, there is a variation of RBE from about 10 to 35. There is a transition region between the Bystander Damage Region and Direct Damage Region of between one and two microdose alpha particle traversals indicating that perhaps two alpha particle “hits” are necessary to produce the direct damage. Extrapolation of underground miners lung cancer risks to human risks at domestic and workplace levels may not be valid

From war to economic recovery: Peace as a public good in Angola

After twenty years of civil war in Angola, and following the country's devastation, transition to a market economy and democracy has finally begun. The end of the war has prompted individual expectations of a real income rise. Within this context, the paper sets out to analyse the willingness of civil servants to help pay (wtp) for consolidation of a public good - peace - in Angola and to calculate the extent to which they are willing to accept (wta) government compensation should it fail to assure this public good. The model used is based on a subjective expected utility function and the sample is taken from high-ranking public administration staff. Results obtained permit one to determine and to interpret wtp and wta by age, sex, hierarchical position, income and number of military conscripts per family.Angola, Military expenditures, Peace as a public good, CVM questionnaire, Willingness to pay, Willingness to accept,

dˉ\bar d and 3^3He production in sNN\sqrt{s_{NN}} = 130 GeV Au + Au collisions

The first measurements of light antinucleus production in Au+Au collisions at RHIC are reported. The observed production rates for antideuterons and antihelions are much larger than in lower energy nucleus-nucleus collisions. A coalescence model analysis of the yields indicates that there is little or no increase in the antinucleon freeze-out volume compared to collisions at SPS energy. These analyses also indicate that the antihelion freeze-out volume is smaller than the antideuteron freeze-out volume