Balanced Xylan Acetylation is the Key Regulator of Plant Growth and Development, and Cell Wall Structure and for Industrial Utilization

Xylan is the most abundant hemicellulose, constitutes about 25–35% of the dry biomass of woody and lignified tissues, and occurs up to 50% in some cereal grains. The accurate degree and position of xylan acetylation is necessary for xylan function and for plant growth and development. The post synthetic acetylation of cell wall xylan, mainly regulated by Reduced Wall Acetylation (RWA), Trichome Birefringence-Like (TBL), and Altered Xyloglucan 9 (AXY9) genes, is essential for effective bonding of xylan with cellulose. Recent studies have proven that not only xylan acetylation but also its deacetylation is vital for various plant functions. Thus, the present review focuses on the latest advances in understanding xylan acetylation and deacetylation and explores their effects on plant growth and development. Baseline knowledge about precise regulation of xylan acetylation and deacetylation is pivotal to developing plant biomass better suited for second-generation liquid biofuel production

Status of Basic Taxonomic Skills in Botanical Articles Related to Azad Jammu and Kashmir, Pakistan: A Review

The problems of synonymy, incorrect species author citation/abbreviation and spelling errors are hampering botanical research around the globe. This article was aimed to quantify the intensity and errors encountered in this regard in published botanical articles pertaining to the Azad Jammu and Kashmir, Pakistan. To address this issue, 100 articles published in 1999-2015 were reviewed. A total of 5460 entries were processed carefully and 1350 species binomials (excluding repititions) were extracted that were linked with 1333 accepted binomials according to theplantlist.org. Out of these accepted species binomials, 1109 were communicated correctly, whereas the remaining 224 binomials were conveyed in the form of 241 synonyms. Average synonyms to accepted binomials ratio for the said timespan was calculated quite high as 13.99%. By taking these conveyed synonyms as our sub sample, we further detected 712 errors related to orthography, author citations and abbreviations. A strong positive and significant Pearson correlation was observed amongst the number of articles published, total numbers of species binomials communicated and the number of synonyms conveyed. These taxonomic errors and nomenclatural issues in botanical articles are causing ambiguity and confusions, thus reducing the reliability and reproducibility of botanical researches. Our results prove modest basic taxonomic skills of authors, dwindling taxonomic understanding and non-updating of regional floras on a periodic basis as primary reasons. We briefly discuss this global issue and its consequences and also document numerous suggestions to mitigate the impacts

Transcriptomic Evidence Reveals Low Gelatinous Layer Biosynthesis in Neolamarckia cadamba after Gravistimulation

Trees can control their shape and resist gravity by producing tension wood (TW), which is a special wood that results from trees being put under stress. TW is characterized by the presence of a gelatinous layer (G layer) and the differential distribution of cell wall polymers. In this study, we investigated whether or not gravistimulation in N. cadamba resulted in TW with an obvious G layer. The results revealed an absence of an obvious G layer in samples of the upper side of a leaning stem (UW), as well as an accumulation of cellulose and a decrease in lignin content. A negligible change in the content of these polymers was recorded and compared to untreated plant (NW) samples, revealing the presence of a G layer either in much lower concentrations or in a lignified form. A transcriptomic investigation demonstrated a higher expression of cell wall esterase- and hydrolase-related genes in the UW, suggesting an accumulation of noncellulosic sugars in the UW, similar to the spectroscopy results. Furthermore, several G-layer-specific genes were also downregulated, including fasciclin-like arabinogalactan proteins (FLA), beta-galactosidase (BGAL) and chitinase-like proteins (CTL). The gene coexpression network revealed a strong correlation between cell-wall-synthesis-related genes and G-layer-synthesis-specific genes, suggesting their probable antagonistic role during G layer formation. In brief, the G layer in N. cadamba was either synthesized in a very low amount or was lignified during an early stage of growth; further experimental validation is required to understand the exact mechanism and stage of G layer formation in N. cadamba during gravistimulation

Exploration of ethno-medicinal knowledge among rural communities of Pearl Valley; Rawalakot, District Poonch Azad Jammu and Kashmir

<div><p>Background</p><p>Medicinal plants are the basic source of health care in the Pearl Valley District Poonch, Azad Jammu, and Kashmir. The basic aim of present study was to record information about the use of plants in herbal preparation and quantification of recorded data.</p><p>Materials and methods</p><p>The research was conducted with the null hypothesis that there was no differential distribution of knowledge among the communities between genders and among different age groups in the study area and across cultural medicinal uses of the plants are similar. To check these hypotheses information about medicinal plants was collected from 46 laypeople and 18 herbalists by using an open ended and semistructured questionnaire. Different ethnobotanical indices were calculated in order to quantify the knowledge on the medicinal plants reported in the study.</p><p>Results</p><p>Our study recorded 136 species of medicinal plants belonging to 45 families with Asteraceae (14 species) as the dominant family of the area. Decoction (26 species), juice and powder (24 species each) were most common methods of preparation. Spearman’s correlation analysis showed that age and gender had the significant effect on both numbers of mentioned species and different uses. A number of known medicinal plants and the number of different uses (H: 38.51; p < 0.001) differ significantly as indicated by Kruskal-Wallis tests. These results showed that the knowledge about the plant varies among different age groups, which were the first hypothesis of the present study. The highest use values (UVs) were reported for <i>Berberis lyceum</i> and <i>Ajuga bracteosa</i> (1.13 each) followed by <i>Abies pindrow</i> (1.03). Highest informant consensus factor (ICF) values were recorded for digestive system diseases (ICF = 0.90) and muscular and skeletal system diseases (ICF = 0.89). The value of Jaccarad index ranged from 6.11 to 32.97 with an average value of 19.84, percentage of similarity was highest between study area and Pir Lasura National Park (34.62%).</p><p>Conclusion</p><p>High similarity might be due to the fact that the communities living in these areas have same sociocultural values and have more opportunities to exchange their traditional knowledge. The present study provides practical evidence about the use of medicinal plants among the inhabitants of the Pearl Valley.</p></div

Novel uses of plant in Pearl Valley Tehsil Rawalakot, District Poonch AJ&K.

<p>Novel uses of plant in Pearl Valley Tehsil Rawalakot, District Poonch AJ&K.</p

Physiological and Transcriptomic Responses of Growth in <i>Neolamarckia cadamba</i> Stimulated by Exogenous Gibberellins

(1) The phytohormones gibberellins (GAs) play a crucial role in plant growth and development, such as seed germination, flowering, fruiting, and stem elongation. Although many biological roles of GAs have been studied intensively, the molecular mechanisms of GAs in woody plants are still unclear. (2) In this study, we investigated the effects of exogenous application of GAs on Neolamarckia cadamba. (3) The height and biomass of N. cadamba increased after 7 days of GA treatment, especially on the second internode. Transcriptome analysis showed that although the majority of genes involved in the GA signaling pathway were up-regulated, the expression of GA20 oxidase (GA20ox) and GA3 oxidase (GA3ox) was down-regulated in the 3 days GA-treated group compared to the CK group. The expression of the cell elongation-related basic helix-loop-helix genes bHLH74 and bHLH49 was up-regulated in the GA-treated group compared with the CK group. Transcriptional expression levels of transcription factors involved in hormone signaling were changed, mainly including bHLH, ethylene response factor (ERF), and WRKY families. In addition, the transcriptional expression level of the key enzymes engaged in the phenylalanine pathway was downregulated after GA treatment. (4) In brief, our findings reveal the physiological and molecular mechanisms of exogenous GA treatment stimulation in N. cadamba

Plants parts use in herbal recipes.

<p>Plants parts use in herbal recipes.</p

Different disease treated by the plants in the study area.

<p>Different disease treated by the plants in the study area.</p

Informant consensus factor (ICF) of Pearl Valley Tehsil Rawalakot, District Poonch AJ&K.

<p>Informant consensus factor (ICF) of Pearl Valley Tehsil Rawalakot, District Poonch AJ&K.</p