Activity of paraoxonase 1 and lipid profile in rats fed cornelian cherry or chokeberry in different types of diet

The study investigates effects of addition of cornelian cherry fruits or chokeberry juice on: activity of paraoxonase 1, lipid profile as well as essential elements in 3 types of diet: control, fructose, high-fat in Wistar rats. For 5 weeks 9 groups of male rats were fed control, fructose, high-fat diets as well control, fructose and high-fat diets enriched with cornelian cherry fruits or chokeberry juice: Activity of paraoxonase 1 was marked both in plasma and liver. Lipid parameters, calcium and magnesium were determined in plasma.Chokeberry juice better than cornelian cherry influenced on activity of paraoxonase 1 and lipid profile in conjunction with different type of diet. Protective effect of antioxidant products such an cornelian cherry or chokeberry depends on type of diet in which is used

Investigation of trace element concentration in diabetic rat's tissues

Diabetes is one of the most frequent diseases in developing countries and thus there is a significant interest in diabetes related studies. It was found that vanadium compounds have glucose-lowering properties in diabetes and therefore it is very important to estimate the vanadium dose in diabetes treatment. On the other hand, the proper estimation of vanadium concentration is important due to side effects that occur in vanadium supplementation. In this study the influence of V(IV) and V(V) compounds with different ligands on the concentration of K, Ca, Mn, Fe, Cu, and Zn in selected rat’s tissues was investigated by means of proton induced X-ray emission technique. As a result of the measurements it was found that the concentration of vanadium depends on the organ. The highest value was determined in spleen while the lowest in pancreas. It was also found that the concentration of other elements depends on the presence of vanadium and its concentration. The most meaningful influence of vanadium presence was on iron concentration in spleen, on copper and zinc in kidney, and on manganese in pancreas

Evaluation of the fat tissue oxidative stress parameters in wistar rats in fructose and high-fat diets with the addition of "Cornus mas L." and "Aronia" juice

Observation of many irregularities in nutrition and disorders of the organ functions, which result from an excessive level of body fat motivates or even imposes the necessity of looking for new ways of prevention and treatment of overweight and obesity. This work presents the effect of food with the addition of fructose (30%), fat (30%) and Cornus mas fruit lyophilizate (10%) and Aronia juice addition on selected parameters of fat tissue oxidative stress in Wistar rats. The work calculated caloric demand for the tested animals fed with the control, fructose and high-fat diet with and without the addition of Cornus mas fruit and Aronia juice. In the tested groups the total antioxidative capacity of fat tissue, determined with the FRAP method, the activity of catalase, superoxide dismutase and the level of glutathione were marked. In plasma of the tested animals biochemical parameters were also marked, such as: total cholesterol, concentration of high-density lipoprotein, the level of triglycerides, concentration of urea and uric acid and glucose levels. It was observed in the conducted research, that an addition of Aronia juice to the high-fat diet caused a significant increase of UA concentration in the AJ group. Cornus mas introduced to the control diet also caused a significant increase of that parameter in the CD group. The conducted experiment shows that the CAT and SOD enzyme activity in the fat tissue homogenate depended on the type of addition introduced to the base feed. The lowest use of feed and the lowest body mass increase was observed in the animal group with high-fat diet and aronia juice (AJ group)

Vanadium methyl-bipyridine organoligand and its influence on energy balance and organs mass

In the treatment of lifestyle diseases, including metabolic syndrome and type 2 diabetes, it is important to lower body mass and fat tissue, and consequently, to increase insulin-sensitivity. Unfortunately, it often happens that low- energy diet which would lower overweight is not observed and, thus, it does not bring the expected effects. This paper discusses the influence of three diets — control, high-fructose, and high-fatty diet — on absorption of energy from food in order to transform it into body mass. The kJ/g ratio which describes this process has been calculated. In the tested diets, the addition of fructose (79.13±2.47 kJ/g) or fat (82.48± 2.28 kJ/g) results in higher transformation of energy into body mass than in the case of control diet (89.60±1.86 kJ/g). The addition of Na[VO(O 2 ) 2 (4,4 ′ -Me 2 -2,2 ′ -bpy)] • 8H 2 O (where 4,4 ′ -Me 2 -2,2 ′ -bpy=4,4 ′ -dimethyl-2,2 ′ -bipyridine) results in sta- tistical increase of that ratio: fruct ose diet (86.88±0.44 kJ/g), fat diet (104.68±3.01 kJ/g), and control diet (115.98±0.56 kJ/g), respectively. Fat diet statistically influences the decrease of kidney mass in comparison to th e other diets. The application of the tested vanadium compound results also in the statistical decrease of the fatty liver caused by fructose and fat diet

Effects of vanadium complexes supplementation on V, Cu, Mn, K, Fe, Zn, and Ca concentration in STZ diabetic rats pancreas

The objective of the study was to assess the effects of Na[V V O(O 2 ) 2 (2,2í-bpy)] ◊ 8 H 2 O (complex 1), Na[V V O(O 2 ) 2 (1,10í-phen)] ◊ 5 H 2 O (complex 2 ), Na[V V O(O 2 ) 2 (4,4í-Me-2,2í-bpy)] ◊ 8 H 2 O (complex 3 ), [ V IV O(SO 4 )(1,10í-phen)] ◊ 2 H 2 O, (complex 4 ), [ V IV O(SO 4 )(2,2í-bpy)] ◊ H 2 O (complex 5 ), where: 2,2í-bpy = 2,2í-bipyridine, 1,10í-phen = 1,10í-phenanthroline, 4,4í-Me-2,2í-bpy = 4,4í-dimethyl-2,2í-bipyridine and a small insulin injection on V, Cu, Mn, K, Fe, Zn, and Ca concentration in the STZ (streptozotocin) diabetic rats pancreas during a 5-week treatment with the tested complexes. In all groups of animals metal concentration in the pancreas was investigated by means of Proton Induced X-ray Emission (PIXE) method. Maximum con- centration of vanadium was observed in the pancreas for complex 5 (1.69 ± 0.09 mg/kg dry weight), lower for complex 3 (1.51 ± 0.10 mg/kg dry weight), and the lowest for complex 1 (1.21 ± 0.27 mg/kg dry weight) sup- plementation. The influence of vanadium administration on other metalsí concentration in the ratsí pancreas was also investigated. All vanadium-tested complexes showed an increase of zinc concentration in the exam- ined pancreas in comparison to the diabetic animals not treated with vanadium. The results were the highest for complex 1 and the lowest for complex 5. The concentration of Fe, Cu, Mn, K and Ca in the pancreas is not evi- dently influenced by administration of the vanadium complexe

Bis(4,4′-dimethyl-2,2′-bipyridine)oxidovanadium(IV) sulfate dehydrate : potential candidate for controlling lipid metabolism?

Vanadium is a trace element mainly connected with regulation of insulin metabolism which is particularly important in diabetes. In recent years, organic complexes of vanadium seem to be more interesting than inorganic salts. Nevertheless, the effect of vanadium on lipid metabolism is still a problematic issue; therefore, the main purpose of this study was to investigate the effect of 3 organic complexes of vanadium such as sodium (2,2′-bipyridine)oxidobisperoxovanadate(V) octahydrate, bis(2,2′-bipyridine)oxidovanadium(IV) sulfate dehydrate, and bis(4,4′-dimethyl-2,2′-bipyridine)oxidovanadium(IV) sulfate dihydrate in conjunction with high-fat as well as control diet in nondiabetes model on the following lipid parameters: total cholesterol, triglycerides, and high density lipoprotein as well as activity of paraoxonase 1. All of these parameters were determined in plasma of Wistar rats. The most significant effect was observed in case of bis(4,4′-dimethyl-2,2′ bipyridine)oxidovanadium(IV) sulfate dehydrate in rats fed with high-fat diet. Based on our research, bis(4,4′-dimethyl-2,2′-bipyridine)oxidovanadium(IV) sulfate dihydrate should be the aim of further research and perhaps it will be an important factor in the regulation of lipid metabolism

Influence of vanadium-organic ligands treatment on selected metal levels in kidneys of STZ rats

The objective of the study was to investigate the effects of five organic vanadium complexes supplement and a small dose of insulin injection on V, Fe, Cu, Zn, Mn, Ca, and K level in the streptozotocin diabetic rat’s kidney during a 5-week treatment with the tested complexes. In all groups of animals, metal level in the lyophilized kidney organs was investigated by means of the proton induced X-ray emission method. Tissue vanadium level was naturally higher in vanadium-treated rats. The maximum level of vanadium was observed in the kidney (x(mean) = 16.6 μg/g). The influence of vanadium administration on other metal level in rat’s tissue was also investigated. Spectacular influence of vanadium action was observed on copper and zinc level in examined tissue

The analysis of Cu, Mn and Zn content in prescription food for special medical purposes and modified milk products for newborns and infants available in Polish pharmacies from toxicological and nutritional point of view

Background: Prescription food for special medical purposes (FSMPs) and modified milk products (MMPs), available in pharmacies, are important for newborns and infants that are not breastfed. In the scientific literature there is a lack of comprehensive studies and corresponding safety assessment of the essential trace elements in these products. Objective: The aim of this article was determination of Cu, Mn and Zn levels in the most frequently available prescription FSMPs (n = 6) and MMPs (n = 6) available in Polish pharmacies. Methods Flame absorption spectrometry (FAAS) following microwave induced digestion (concentrated nitric acid) was applied to determine the levels of the elements in the products. Results: Our studies are based on a triple approach (1) the “raw results” of Cu, Mn and Zn levels (products in powdered form), (2) single intake - the level of each essential trace element consumed in one portion, (3) the daily intake depending on age and weight including comparison with Adequate Intake established by European Food Safety Authority. Conclusion: The results show the occurrence of differences between the manufacturer’s declared composition and the finished product for consumption. The prescription FSMPs in comparison to MMPs available in Polish pharmacies contain similar levels of Cu, Mn and Zn. Our results show additionally that all of the products do not represent a health hazard to the newborns and infants. This is a pioneer study in terms of the safety assessment, and quality of prescription FSMPs and MMPs available in Polish pharmacies from toxicological and nutritional point of view