Magnetic techniques for the isolation and purification of proteins and peptides

Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques

Magnetic fluid modified peanut husks as an adsorbent for organic dyes removal

AbstractMagnetically responsive nanocomposite materials, prepared by modification of diamagnetic materials by magnetic fluids (ferrofluids), have already found many important applications in various areas of biosciences, medicine, biotechnology, environmental technology etc. Ferrofluid modified biological waste (peanut husks) has been successfully used for the separation and removal of water soluble organic dyes and thus this low cost adsorbent could be potentially used for waste water treatment

Izolacija lizozima iz bjelanjka kokošjeg jajeta magnetskom kationskom izmjenom

Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96 %) with specific activity similar to that of commercial lysozyme preparations was obtained in both cases. Carboxymethyl-based cation exchanger exhibited substantially higher capacity for lysozyme; maximum adsorption capacity was 138 mg/mL. The lysozyme-depleted egg white can be used in the same way as the routinely used egg white because no dilution of this material was necessary during the purification process.Dva magnetska kationska izmjenjivača od celuloznih vlakana velike poroznosti (Iontosorb MG CM 100 i Iontosorb MG SHP 100) upotrijebljena su da bi se jednostavno izolirao lizozim iz nerazrijeđenoga kokošjeg jajeta. U oba slučaja dobiven je lizozim velike čistoće (>96 %) čija je specifična aktivnost slična onoj komercijalnih lizozima. Kationski izmjenjivač na bazi karboksimetila imao je veću sposobnost adsorpcije lizozima (maksimalni kapacitet adsorpcije bio je 138 mg/mL). Nakon izdvajanja lizozima, bjelanjak se i dalje može upotrijebiti

New magnetically responsive yeast-based biosorbent for the efficient removal of water-soluble dyes

Fodder yeast (Kluyveromyces fragilis) cells were magnetically modified by a contact with thewater-based magnetic fluid in order to prepare a new type of magnetically responsive biocomposite material. This procedure enabled a simple separation of modified cells by means of commercially available magnetic separators or strong permanent magnets. It allows using the prepared material as a new inexpensive magnetic affinity adsorbent for the removal of water-soluble dyes. Magnetically modified cells were characterized by means of magnetic and microscopy methods. Both isolated magnetic nanoparticles and aggregates of particles were present on the cell surface. The prepared material displayed a superparamagnetic behavior at room temperature, with a transition to a blocked state at TB 180K for the applied magnetic field H=50Oe. Seven dyes (crystal violet, amido black 10B, congo red, Saturn blue LBRR, Bismarck brown, acridine orange and safranin O) were used to study the adsorption process. The dyes adsorption could be described with the Langmuir isotherm. The maximum adsorption capacities ranged between 29.9 (amido black 10B) and 138.2 (safranin O) mg of dye per g of dried magnetically modified cells

P(3HB) Based Magnetic Nanocomposites: Smart Materials for Bone Tissue Engineering

The objective of this work was to investigate the potential application of Poly(3-hydroxybutyrate)/magnetic nanoparticles, P(3HB)/MNP, and Poly(3-hydroxybutyrate)/ferrofluid (P(3HB)/FF) nanocomposites as a smart material for bone tissue repair. The composite films, produced using conventional solvent casting technique, exhibited a good uniform dispersion of magnetic nanoparticles and ferrofluid and their aggregates within the P(3HB) matrix. The result of the static test performed on the samples showed that there was a 277% and 327% increase in Young’s modulus of the composite due to the incorporation of MNP and ferrofluid, respectively. The storage modulus of the P(3HB)MNP and P(3HB)/FF was found to have increased to 186% and 103%, respectively, when compared to neat P(3HB). The introduction of MNP and ferrofluid positively increased the crystallinity of the composite scaffolds which has been suggested to be useful in bone regeneration. The total amount of protein absorbed by the P(3HB)/MNP and P(3HB)/FF composite scaffolds also increased by 91% and 83%, respectively, with respect to neat P(3HB). Cell attachment and proliferation were found to be optimal on the P(HB)/MNP and P(3HB)/FF composites compared to the tissue culture plate (TCP) and neat P(3HB), indicating a highly compatible surface for the adhesion and proliferation of the MG-63 cells. Overall, this work confirmed the potential of using P(3HB)/MNP and P(3HB)/FF composite scaffolds in bone tissue engineering

Ferrofluid modified Saccharomyces cerevisiae cells for biocatalysis. Food Res

a b s t r a c t An inexpensive and non-toxic magnetically responsive biocatalyst was prepared by ferrofluid modification of Saccharomyces cerevisiae cells. The prepared biocatalyst enabled efficient decomposition of hydrogen peroxide (HP) and sucrose conversion, due to the presence of active intracellular catalase and invertase. HP was efficiently decomposed in water solutions up to 2% concentration, leaving very low residual HP concentration after treatment. The biocatalyst was stable; the same catalytic activity was observed after one month storage at 4°C