Faculty of Food Technology and Biotechnology, University of Zagreb
Abstract
Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96 %) with specific activity similar to that of commercial lysozyme preparations was obtained in both cases. Carboxymethyl-based cation exchanger exhibited substantially higher capacity for lysozyme; maximum adsorption capacity was 138 mg/mL. The lysozyme-depleted egg white can be used in the same way as the routinely used egg white because no dilution of this material was necessary during the purification process.Dva magnetska kationska izmjenjivača od celuloznih vlakana velike poroznosti (Iontosorb MG CM 100 i Iontosorb MG SHP 100) upotrijebljena su da bi se jednostavno izolirao lizozim iz nerazrijeđenoga kokošjeg jajeta. U oba slučaja dobiven je lizozim velike čistoće (>96 %) čija je specifična aktivnost slična onoj komercijalnih lizozima. Kationski izmjenjivač na bazi karboksimetila imao je veću sposobnost adsorpcije lizozima (maksimalni kapacitet adsorpcije bio je 138 mg/mL). Nakon izdvajanja lizozima, bjelanjak se i dalje može upotrijebiti
