9 research outputs found
Nursing Case Report in Patient after right Breast Reconstruction with Abdominal Flap at the Departement of Plastic Surgery
<p>A: CLDN2; B: CLDN12; C: OCLN; D: ZO1.</p
Effect of mucosal addition of 20 mM TAP in the proximal jejunum.
<p>TAP, 2,4,6 triaminopyrimidin. Effect on tissue conductance (G<sub>t</sub>) (a). Inhibition of G<sub>t</sub> statistically quantified by paired t-test (b).</p
Expression of Tight Junction Proteins and Cadherin 17 in the Small Intestine of Young Goats Offered a Reduced N and/or Ca Diet
<div><p>Diets fed to ruminants should contain nitrogen (N) as low as possible to reduce feed costs and environmental pollution. Though possessing effective N-recycling mechanisms to maintain the N supply for rumen microbial protein synthesis and hence protein supply for the host, an N reduction caused substantial changes in calcium (Ca) and phosphate homeostasis in young goats including decreased intestinal transepithelial Ca absorption as reported for monogastric species. In contrast to the transcellular component of transepithelial Ca transport, the paracellular route has not been investigated in young goats. Therefore, the aim of the present study was to characterise the effects of dietary N and/or Ca reduction on paracellular transport mechanisms in young goats. Electrophysiological properties of intestinal epithelia were investigated by Ussing chamber experiments. The expression of tight junction (TJ) and adherens junction (AJ) proteins in intestinal epithelia were examined on mRNA level by <i>q</i>PCR and on protein level by western blot analysis. Dietary N reduction led to a segment specific increase in tissue conductances in the proximal jejunum which might be linked to concomitantly decreased expression of cadherin 17 mRNA. Expression of occludin (OCLN) and zonula occludens protein 1 was increased in mid jejunal epithelia of N reduced fed goats on mRNA and partly on protein level. Reduced dietary Ca supply resulted in a segment specific increase in claudin 2 and claudin 12 expression and decreased the expression of OCLN which might have been mediated at least in part by calcitriol. These data show that dietary N as well as Ca reduction affected expression of TJ and AJ proteins in a segment specific manner in young goats and may thus be involved in modulation of paracellular Ca permeability.</p></div
Effect of mucosal addition of 20 mM TAP in the mid jejunum.
<p>TAP, 2,4,6 triaminopyrimidin. Effect on tissue conductance (G<sub>t</sub>) (a). Inhibition of G<sub>t</sub> statistically quantified by paired t-test (b).</p
Initial and final body weight as well as daily weight gain of growing goats receiving an N and/or Ca reduced diet.
<p>Initial and final body weight as well as daily weight gain of growing goats receiving an N and/or Ca reduced diet.</p
Representative single immunohistochemistry for CLDN2 in mid jejunal epithelial paraffin sections of (N+/Ca+) goats.
<p>CLDN2 is detectable apically (arrows) and in lateral membranes of enterocytes (arrowheads) in crypts. No unspecific staining was detected in the negative control (insert). Scale bars: 100 μm.</p
RNA expression of tight junction proteins normalised to GAPDH in the proximal and mid jejunum and ileum of goats fed an N and/or Ca reduced diet.
<p>RNA expression of tight junction proteins normalised to GAPDH in the proximal and mid jejunum and ileum of goats fed an N and/or Ca reduced diet.</p
Electrophysiological properties of proximal, mid jejunal and ileal epithelia of young goats during dietary N and/or Ca reduction.
<p>Electrophysiological properties of proximal, mid jejunal and ileal epithelia of young goats during dietary N and/or Ca reduction.</p