PLATELET AGGREGATION AFTER EXPERIMENTAL BURN INJURY AND THERAPY

Based on data concerning the coagulation changes after thermal injury the authors studied platelet aggregation after burn and its treatment. Severe thermal injury of Ha and lib degree was inflicted in white male rats (200 ±20 g b. m.) under aether anaesthesia. It ranged over 17,5 ± 2,5 % of the body surface. The animals were divided into the following groups: 1) burned non-treated; 2) burned and treated with Sol. Hartmanni (Hr), 3) burned and treated with Hemodex (Hx), and 4) controls. The treatment was intraperitoneally carried out immediately after the injury as well as on 6th and 24th hour after it. An elevation of the platelet aggregation for all the groups on the 24th hour after the injury was established

PROTEIN AND MINERAL METABOLISM IN LIMITED PROFOUND BURNS

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SOME THROMBOCYTIC AND ERYTHROCYTIC PARAMETERS AFTER BURNING (EXPERIMENTAL INVESTIGATIONS)

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NF2/Merlin mediates contact-dependent inhibition of EGFR mobility and internalization via cortical actomyosin

The proliferation of normal cells is inhibited at confluence, but the molecular basis of this phenomenon, known as contact-dependent inhibition of proliferation, is unclear. We previously identified the neurofibromatosis type 2 (NF2) tumor suppressor Merlin as a critical mediator of contact-dependent inhibition of proliferation and specifically found that Merlin inhibits the internalization of, and signaling from, the epidermal growth factor receptor (EGFR) in response to cell contact. Merlin is closely related to the membrane–cytoskeleton linking proteins Ezrin, Radixin, and Moesin, and localization of Merlin to the cortical cytoskeleton is required for contact-dependent regulation of EGFR. We show that Merlin and Ezrin are essential components of a mechanism whereby mechanical forces associated with the establishment of cell–cell junctions are transduced across the cell cortex via the cortical actomyosin cytoskeleton to control the lateral mobility and activity of EGFR, providing novel insight into how cells inhibit mitogenic signaling in response to cell contact

Genetic diversity and host alternation of the egg parasitoid Oencyrtus pityocampae between the pine processionary moth and caper bug

Research ArticleThe increased use of molecular tools for species identification in recent decades revealed that each of many apparently generalist parasitoids are actually a complex of morphologically similar congeners, most of which have a rather narrow host range. Ooencyrtus pityocampae (OP), an important egg parasitoid of the pine processionary moth (PPM), is considered a generalist parasitoid. OP emerges from PPM eggs after winter hibernation, mainly in spring and early summer, long before the eggs of the next PPM generation occurs. The occurrence of OP in eggs of the variegated caper bug (CB) Stenozygum coloratum in spring and summer suggests that OP populations alternate seasonally between PPM and CB. However, the identity of OP population on CB eggs seemed uncertain; unlike OP-PPM populations, the former displayed apparently high male/female ratios and lack of attraction to the PPM sex pheromone. We studied the molecular identities of the two populations since the morphological identification of the genus Ooencyrtus, and OP in particular, is difficult. Sequencing of COI and ITS2 DNA fragments and AFLP analysis of individuals from both hosts revealed no apparent differences between the OP-PPM and the OP-CB populations for both the Israeli and the Turkish OPs, which therefore supported the possibility of host alternation. Sequencing data extended our knowledge of the genetic structure of OP populations in the Mediterranean area, and revealed clear separation between East and West Mediterranean populations. The overall level of genetic diversity was rather small, with the Israeli population much less diverse than all others; possible explanations for this finding are discussed. The findings support the possibility of utilizing the CB and other hosts for enhancing biological control of the PPMinfo:eu-repo/semantics/publishedVersio

Controlling as a comprehensive system for early warning and development process and decision making in a crisis

The article is considered as controlling system for managerial decision making, which allows you to develop mechanisms of action for achieving the goal. The mechanism and controlling tools as an integrated system for early warning and management decisions

Our trial in problem-oriented teaching for pathophysiology

The decision for clinical cases during pathophysiology is linked with the introduction of problem-oriented learning in pathophysiology through the next few years. "Clinical cases" from general medicine (arterial hypertension, diabetes mellitus, pancreatic insufficiency, cerebral vascular disease, etc) are examined in the frame work of two sessions. On the basis of a short description of the patient the students develop hypotheses for the possible causes which lead to the pathogenic connection between the cause, the afflicted structure and function, symptom, syndrome and illness. The difficulty which the students most of ten face is connected with the basis of the hypothesis, which they must relate knowledge from the fundamental sciences and general pathophysiology in order to solve concrete clinical cases. Analyses of the results are made of the students' difficulties during solving of clinical cases as well as the necessary path for over coming it. On the basis of our investigation, we consider that the decision of clinical cases makes learning pathophysiology more engaging for the student, creating conditions for in dependent preparation and local thinking, which are necessary of the students for solving patient problems.Scripta Scientifica Medica 2010;42(1):67-6

Improving the education in pathophysiology by bringing in clinical cases during seminar lessons

Nowadays, high quality education is the center of attention in every medical school. Searching for new ways to introduce the educational units is a natural drift for every discipline. Introducing new educational technique: "Solving of Clinical Case", aims at building a spontaneous bridge between preclinical knowledge and future work in the hospital in immediate and unattended contact with patients. Clinical case solving is a prerequisite for increasing the motivation of students, enhancing logical thinking and stimulating the student for additional investigation at home. The clinical case is based on real patients and the history of their diseases and it is related to the topic of the exercise. It includes the whole data from the moment the patient is seen by doctor for the first time until definitive diagnosis is formulated and medication prescribed. The clinical case is divided in two parts, discussed in two subsequent seminar classes. During the first part the tutor does not give any additional information about the case, he just guides the discussion. The students work by themselves, formulate hypothesis and argument them by building logical connections between causes and results, using the model: causes (and conditions) - altered structure - harmed function - clinical symptom - set of symptoms (syndromes) - disease. At the end of the first part hypothesis are rearranged by their probability. The students may use the time between the two parts to find additional information on the topic and gain some knowledge on the hypothesis that have been discussed, using either traditional forms of education - textbooks, monographs, lectures, original scientific issues and reviews or any kind of source including the www. The second part is used for discussing what kind of laboratory, functional or instrumental tests are still needed to prove or exclude the hypothetical diagnosis. The way it is created, every clinical case emphasizes not only on the biomedical side of the patient's problem, but also the socio-judicial aspects.Scripta Scientifica Medica 2008; 40(2): 117-120