Response of wheat to additional nitrogen fertilizer application after pig slurry on over-fertilized soils

Pig slurry is a valuable nutrient resource but constitutes a waste disposal problem in areas of high animal density. In the semiarid area of Pla d’Urgell, in the Ebro Valley, North-East Spain, irrigated crops receive large amounts of nutrients in the form of manure and mineral fertilizers. We studied the effect of pig slurry and additional side-dress mineral fertilizers on irrigated wheat, Triticum aestivum L., on a coarse loam soil, with high soil P and K levels. Yields increased by 62.3% when using pig slurry. The application of ammonium sulfate nitrate sidedress did not significantly increase wheat production. The average apparent recoveries were higher for potassium (88.7%) than for nitrogen (51.3%) and phosphorus (36.3%). Greater amounts of soil NO3-N were measured over the four growing seasons, which was consistent with the amount of N applied. Macronutrient and micronutrient uptake was significant higher for pig slurry treatments, but only small differences were found between the pig slurry and pig slurry plus ammonium sulfate nitrate treatments. The unfertilized treatment showed significantly lower soil P, K, Cu and Zn content than pig slurry treatments; 34%, 21%, 34%, and 26% respectively. These findings could be used to develop a nutrient management plan based on knowledge of soil test results and crop nutrient removal. This could help to improve the use of pig slurry and mineral fertilizers on limited available land areas and prevent the accumulation of potentially toxic elements in soils and the export of nutrients through agricultural drainage

Surface sterilization method for reducing microbial contamination of field grown strawberry explants intended for in vitro culture

An effective disinfection method for strawberry (Fragaria x ananassa Duch.) cv. Senga Sengana micropropagation using runner tips and nodal segments as explants was developed. The explants were surface sterilized with different sterilants for different durations. The present studies on the effect of different regimes of sterilization revealed that maximum aseptic cultures were obtained from both explants runner tips and nodal segments when treated with 1.5% sodium hypochlorite for 20 min plus ethyl alcohol 70% for 30 s, but the surviving percentage was less because this treatment resulted in necrosis and tissue injury of explants. However, mercuric chloride (0.1%) for 4 min resulted in less percentage of aseptic cultures but gave highest percentage of surviving explants as most of researchers have found that a single sterilant is more effective than the combination. Surface sterilization with mercuric chloride (0.1%) for 4 min was the optimum duration which resulted in highest percentage of explant survival.Keywords: In vitro, senga sengana, strawberry, sterilizationAfrican Journal of Biotechnology Vol. 12(39), pp. 5749-575

EVALUATION OF ROOT AND LEAF OF ABUTILON THEOPHRASTI MEDIK FOR ANTIFUNGAL ACTIVITY

ABSTRACTThe investigation was aimed to carry out the antifungal activities of the methanol and aqueous extracts of Abutilon theophrasti against four fungalstrains (Microsporum gypseum, Penicillium spp., Fusarium spp., Aspergillus spp.). Antifungal activity of the extracts was determined by the discdiffusion method against various fungi with soubred dextrose agar as media. Methanolic extracts of A. theophrasti showed promising antifungalactivity compared to aqueous extracts against selected fungal species. Methanolic extract of leaf displayed the highest activity with zone of inhibition14 mm against Penicillium spp. which were totally concentration dependent; however, a poor activity was seen in aqueous extracts. Methanolicextracts of root displayed good antifungal activity, while as no activity was seen in aqueous extracts of root. The results obtained in the study showthat methanolic leaf extracts possess phytoconstituents with potent antifungal activity.Keywords: Abutilon, Methanol, Rhizopus, Penicillium

The nuclear factor erythroid 2-related factor 2/p53 axis in breast cancer

One of the most important factors involved in the response to oxidative stress (OS) is the nuclear factor erythroid 2-related factor 2 (Nrf2), which regulates the expression of components such as antioxidative stress proteins and enzymes. Under normal conditions, Kelch-like ECH-associated protein 1 (Keap1) keeps Nrf2 in the cytoplasm, thus preventing its translocation to the nucleus and inhibiting its role. It has been established that Nrf2 has a dual function; on the one hand, it promotes angiogenesis and cancer cell metastasis while causing resistance to drugs and chemotherapy. On the other hand, Nrf2 increases expression and proliferation of glutathione to protect cells against OS. p53 is a tumour suppressor that activates the apoptosis pathway in aging and cancer cells in addition to stimulating the glutaminolysis and antioxidant pathways. Cancer cells use the antioxidant ability of p53 against OS. Therefore, in the present study, we discussed function of Nrf2 and p53 in breast cancer (BC) cells to elucidate their role in protection or destruction of cancer cells as well as their drug resistance or antioxidant properties

High-Resolution SMAP-Derived Root-Zone Soil Moisture Using an Exponential Filter Model Calibrated per Land Cover Type

Root-zone soil moisture (RZSM) plays a key role for most water and energy budgets, as it is particularly relevant in controlling plant transpiration and hydraulic redistribution. RZSM data is needed for a variety of different applications, such as forecasting crop yields, improving flood predictions and monitoring agricultural drought, among others. Remote sensing provides surface soil moisture (SSM) retrievals, whose key advantage is the large spatial coverage on a systematic basis. This study tests a simple method to retrieve RZSM estimates from high-resolution SSM derived from SMAP (Soil Moisture Active Passive). A recursive exponential filter using a time constant τ is calibrated per land cover type, which uses as an intermediate step a long-term ISBA-DIF (Interaction Soil Biosphere Atmosphere—Diffusion scheme) dataset over an area located in Catalonia, NE of Spain. The τ values thus obtained are then used as an input to the same recursive exponential filter, to derive 1 km resolution RZSM estimates from 1 km SMAP SSM, which are obtained from the original data by downscaling to a 1 km resolution, through the DISPATCH (DISaggregation based on a Physical and Theoretical scale CHange) methodology. The results are then validated with scaled in situ observations at different depths, over two different areas, one representative of rainfed crops, and the other of irrigated crops. In general, the estimates agree well with the observations over the rainfed crops, especially at a 10 cm and 25 cm depth. Nash–Sutcliffe (NS) scores ranging between 0.33 and 0.58, and between 0.37 and 0.56 have been found, respectively. Correlation coefficients for these depths are high, between 0.76 and 0.91 (10 cm), and between 0.71 and 0.90 (25 cm). For the irrigated sites, results are poorer (partly due to the extremely high heterogeneity present), with NS scores ranging between −2.57 and 0.16, and correlations ranging between −0.56 and 0.48 at 25 cm. Given the strong correlations and NS scores found in the surface, the sensitivity of the filter to different τ values was investigated. For the rainfed site, it was found, as expected, with increasing τ, increasing NS and correlations with the deeper layers, suggesting a better coupling. Nevertheless, a strong correlation with the surface (5 cm) or shallower depths (10 cm) observed over certain sites indicates a certain lack of skill of the filter to represent processes which occur at lower levels in the SM column. All in all, a calibration accounting for the vegetation was shown to be an adequate methodology in applying the recursive exponential filter to derive the RZSM estimates over large areas. Nevertheless, the relative shallow surface at which the estimates correlate in some cases seem to indicate that an effect of evapotranspiration in the profile is not well captured by the filter.This research was funded by the Torrres Quevedo program of the Spanish Science Ministry, MICINN (grant number PTQ-16-08766)