306 research outputs found
Nursing Advising Using a MOOC: A Case Study
Advanced technology has moved online courses from being available to exclusively to elite students to literally being open to the general public. The proliferation of Massive Open Online Courses (MOOCs) has led to expanding public access to a wide range of information including careers in health care fields. Our group developed a MOOC to assist people from around the world who are interested in pursuing a career in nursing get the information they need to be successful in the nursing program and in the profession of nursing. In this article, we describe course content, who the students were who enrolled in the free MOOC, and the course outcomes we developed. In addition, we discuss lessons learned and provide recommendations to assist others seeking to develop a MOOC for career advising
New Measurement of the Cosmic-Ray Positron Fraction from 5 to 15 GeV
We present a new measurement of the cosmic-ray positron fraction at energies
between 5 and 15 GeV with the balloon-borne HEAT-pbar instrument in the spring
of 2000. The data presented here are compatible with our previous measurements,
obtained with a different instrument. The combined data from the three HEAT
flights indicate a small positron flux of non-standard origin above 5 GeV. We
compare the new measurement with earlier data obtained with the HEAT-e+-
instrument, during the opposite epoch of the solar cycle, and conclude that our
measurements do not support predictions of charge sign dependent solar
modulation of the positron abundance at 5 GeV.Comment: accepted for publication in PR
Characterization and expression analysis of the groESL operon of Bartonella bacilliformis
The groESL operon of Bartonella bacilliformis, a facultative intracellular, Gram-negative bacterium and etiologic agent of Oroya Fever, was characterized. Sequence analysis revealed an operon containing two genes of 294 (groES) and 1632 nucleotides (groEL) separated by a 55-nt intergenic spacer. The operon is preceded by a 72-nt ORF (ORF1) that encodes a hypothetical protein with homology to a portion of the HrcA repressor for groESL. A divergent fumarate hydratase C (fumC) gene lies further upstream. Deduced amino acid sequences for B. bacilliformis GroEL and GroES revealed a high degree of identity with homologues from other Bartonella and alpha-Protebacteria. A single transcriptional start site (TSS) was mapped 79 nucleotides upstream of the groES start codon, regardless of incubation temperature. The TSS was located immediately 5\u27 to a potential controlling inverted repeat of chaperonin expression (CIRCE) element and is preceded by a sigma70-like promoter. The operon is followed by a predicted rho-independent transcriptional terminator. Northern blot analysis indicated that groES and groEL are co-transcribed as a single mRNA of approximately 2.4 kb. A 6-h time course analysis by qRT-PCR showed that groEL expression increases 1.3-fold within 30 min of a temperature upshift from 30 to 37 degrees C, with maximum transcription reached after 60 min (approximately 4.3-fold), followed by a steady decrease to background (30 degrees C) transcription levels by 6 h. Western blot analysis revealed a 1.4- and 1.5-fold increase in GroEL synthesis following a temperature upshift or by inhibiting DNA supercoiling with coumermycin A1, respectively. Functional expression and complementation of temperature-sensitive Escherichia coli groES or groEL mutants with the cloned operon allowed them to grow at otherwise restrictive temperatures
Elemental energy spectra of cosmic rays measured by CREAM-II
We present new measurements of the energy spectra of cosmic-ray (CR) nuclei
from the second flight of the balloon-borne experiment CREAM (Cosmic Ray
Energetics And Mass). The instrument (CREAM-II) was comprised of detectors
based on different techniques (Cherenkov light, specific ionization in
scintillators and silicon sensors) to provide a redundant charge identification
and a thin ionization calorimeter capable of measuring the energy of cosmic
rays up to several hundreds of TeV. The data analysis is described and the
individual energy spectra of C, O, Ne, Mg, Si and Fe are reported up to ~ 10^14
eV. The spectral shape looks nearly the same for all the primary elements and
can be expressed as a power law in energy E^{-2.66+/-0.04}. The nitrogen
absolute intensity in the energy range 100-800 GeV/n is also measured.Comment: 4 pages, 3 figures, presented at ICRC 2009, Lodz, Polan
Measurements of cosmic-ray energy spectra with the 2nd CREAM flight
During its second Antarctic flight, the CREAM (Cosmic Ray Energetics And
Mass) balloon experiment collected data for 28 days, measuring the charge and
the energy of cosmic rays (CR) with a redundant system of particle
identification and an imaging thin ionization calorimeter. Preliminary direct
measurements of the absolute intensities of individual CR nuclei are reported
in the elemental range from carbon to iron at very high energy.Comment: 4 pages, 3 figures, presented at XV International Symposium on Very
High Energy Cosmic Ray Interactions (ISVHECRI 2008
Energy spectra of cosmic-ray nuclei at high energies
We present new measurements of the energy spectra of cosmic-ray (CR) nuclei
from the second flight of the balloon-borne experiment Cosmic Ray Energetics
And Mass (CREAM). The instrument included different particle detectors to
provide redundant charge identification and measure the energy of CRs up to
several hundred TeV. The measured individual energy spectra of C, O, Ne, Mg,
Si, and Fe are presented up to eV. The spectral shape looks
nearly the same for these primary elements and it can be fitted to an power law in energy. Moreover, a new measurement of the absolute
intensity of nitrogen in the 100-800 GeV/ energy range with smaller errors
than previous observations, clearly indicates a hardening of the spectrum at
high energy. The relative abundance of N/O at the top of the atmosphere is
measured to be (stat.)(sys.) at 800
GeV/, in good agreement with a recent result from the first CREAM flight.Comment: 32 pages, 10 figures. Accepted for publication in Astrophysical
Journa
Cosmic-Ray Proton and Helium Spectra from the First CREAM Flight
Cosmic-ray proton and helium spectra have been measured with the
balloon-borne Cosmic Ray Energetics And Mass experiment flown for 42 days in
Antarctica in the 2004-2005 austral summer season. High-energy cosmic-ray data
were collected at an average altitude of ~38.5 km with an average atmospheric
overburden of ~3.9 g cm. Individual elements are clearly separated with
a charge resolution of ~0.15 e (in charge units) and ~0.2 e for protons and
helium nuclei, respectively. The measured spectra at the top of the atmosphere
are represented by power laws with a spectral index of -2.66 0.02 for
protons from 2.5 TeV to 250 TeV and -2.58 0.02 for helium nuclei from 630
GeV/nucleon to 63 TeV/nucleon. They are harder than previous measurements at a
few tens of GeV/nucleon. The helium flux is higher than that expected from the
extrapolation of the power law fitted to the lower-energy data. The relative
abundance of protons to helium nuclei is 9.1 0.5 for the range from 2.5
TeV/nucleon to 63 TeV/nucleon. This ratio is considerably smaller than the
previous measurements at a few tens of GeV/nucleon.Comment: 20 pages, 4 figure
Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion
The fidelity of DNA polymerases depends on conformational changes that promote the rejection of incorrect nucleotides before phosphoryl transfer. Here, we combine single-molecule FRET with the use of DNA polymerase I and various fidelity mutants to highlight mechanisms by which active-site side chains influence the conformational transitions and free-energy landscape that underlie fidelity decisions in DNA synthesis. Ternary complexes of high fidelity derivatives with complementary dNTPs adopt mainly a fully closed conformation, whereas a conformation with a FRET value between those of open and closed is sparsely populated. This intermediate-FRET state, which we attribute to a partially closed conformation, is also predominant in ternary complexes with incorrect nucleotides and, strikingly, in most ternary complexes of low-fidelity derivatives for both correct and incorrect nucleotides. The mutator phenotype of the low-fidelity derivatives correlates well with reduced affinity for complementary dNTPs and highlights the partially closed conformation as a primary checkpoint for nucleotide selection
A new measurement of the altitude dependence of the atmospheric muon intensity
We present a new measurement of atmospheric muons made during an ascent of
the High Energy Antimatter Telescope balloon experiment. The muon charge ratio
mu+/mu- as a function of atmospheric depth in the momentum interval 0.3-0.9
GeV/c is presented. The differential mu- intensities in the 0.3-50 GeV/c range
and for atmospheric depths between 4-960 g/cm^2 are also presented. We compare
these results with other measurements and model predictions. We find that our
charge ratio is ~1.1 for all atmospheric depths and is consistent, within
errors, with other measurements and the model predictions. We find that our
measured mu- intensities are also consistent with other measurements, and with
the model predictions, except at shallow atmospheric depths.Comment: To appear in Physical Review
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