Molecular characterization of a Chinese variant of the Flury-LEP strain

The entire genome of rabies virus vaccine strain Flury-LEP-C, a Chinese variant of the rabies virus vaccine strain Flury-LEP, was sequenced. The overall length of the genome of Flury-LEP-C strain was 11 924 nucleotides (nt), comprising a leader sequence of 58 nt, nucleoprotein (N) gene of 1353 nt, phosphoprotein (P) gene of 894 nt, matrix protein (M) gene of 609 nt, glycoprotein (G) gene of 1575 nt, RNA-dependent RNA polymerase (RdRp, L) gene of 6384 nt, and a trailer region of 70 nt. There was TGAAAAAAA (TGA7) consensus sequence in the end of each gene in Flury-LEP-C genome, except G gene which had a GAGAAAAAAA sequence in the end of the non-coding G-L region. There were AACAYYYCT consensus start signal close to the TGA7. Flury-LEP-C has 310 nucleotides more than HEP-Flury in G-L intergenic region. The analysis showed that the residue at 333 of the mature G protein was Arg, which was reported to be related to pathogenicity. Compared with FluryLEP, there were 19 different amino acids (AAs) in five proteins of Flury-LEP-C, including 15 AAs which were identical with corresponding residues of Hep-Flury, and 4 AAs which were neither identical with the residues of FluryLEP nor with the residues of Hep-Flury. The results showed the topology of the phylogenetic trees generated by two protein sequences were similar. It was demonstrated that HN10, BD06, FJ009, FJ008, D02, D01, F04, F02 have a close relationship to CTN-1 and CTN181, and MRV was closely related to Flury-LEP, HEP-Flury and Flury-LEP-C

Determinants of whistleblowing intention : evidence from the South Korean government

In 2001, the South Korean government passed the Anti-Corruption Act, which provides whistleblower protection in the public sector. The system of protections and rewards was strengthened in 2011 by the Act on the Protection of Public Interest Whistleblowers. Although these laws ensure immunity—and even financial incentives—for whistleblowers, whistleblowing is still not a straightforward task. Based on a survey of 5706 public officials in central government, this study examines how a range of factors influence whistleblowing intention: attitude; knowledge; colleague support; organizational support; and protection against retaliation. A number of demographic variables, relating to gender; marital status; length of tenure; duty; and position type are used as controls. The results of the ordered probit regression analysis show all of the independent variables to have a significant positive effect on whistleblowing intention. However, colleague support and organizational support have the biggest effects, while perceived protection against retaliation has the smallest. This suggests that there is a need for future government efforts to build upon the available legal protections by focusing on creating a supportive culture among colleagues and in the organization more generally