Individual Professional Practice in the Company

Import 23/08/2017Cílem této bakalářské práce je popsat absolvování odborné praxe ve firmě HS Interactive s.r.o. Praxe byla zaměřena na vývoj mobilní aplikace pro operační systém Android. Aplikace je mobilním klientem pro sociální síť MatchToMe. V úvodu popisuji důvody, které vedly k výběru odborné praxe. Dále se věnuji úkolům, které mi byly zadány s jejich implementací a postupem řešení problémů, které se objevily při vývoji. Závěr práce je věnován zhodnocení získaných zkušeností a dosažených výsledků.Purpose of this bachelor thesis is to describe a professional practice in company HS Interactive s.r.o. Practice was focused on the development of mobile application for the operating system Android. The application is a mobile client for social network MatchToMe. In the introduction I describe reasons that led to the selection of professional practice. Then I describe tasks that I have been awarded with their implementations and process of solution issues that have emerged during development. The conclusion of thesis is dedicated to the evaluation of the experience gained and the results achieved.440 - Katedra telekomunikační technikyvýborn

Dynamic microRNAome profiles in the developing porcine liver

<p>Increasing evidence suggests that micro (mi)RNAs play important roles in various biological process. To evaluate the roles of miRNA in the porcine liver, we investigated the dynamic profiles of microRNAomes using liver tissue from pigs during the embryonic period (embryonic day 90), weaning stage (postnatal day 30), and adult stage (7 years old). A total of 186 unique miRNAs were differentially expressed during liver development. We also identified that 17, 13, and 6 miRNAs were specifically abundant at embryonic day 90, postnatal day 30, and at 7 years, respectively. Besides regulating basic cellular roles in development, miRNAs expressed at the three developmental stages also participated in regulating “embryonic liver development,” “early hepatic growth and generating a functioning liver,” and “energy metabolic processes,” respectively. Our study indicates that miRNAs are extensively involved in liver development, and provides a valuable resource for the further elucidation of miRNA regulatory roles during liver development.</p> <p>miRNAs are extensively involved in liver development, especially for these stage-enriched miRNAs.</p

Supplementary table 2.

Supplementary table 2. Detailed information on single-copy orthologous PCGs across 15 vertebrate species.</p

Supplementary figure.1

Supplementary figure.1 PCA plot of PC1 versus PC3 and PC2 versus PC3 based on the expression levels of single-copy orthologous PCGs among 15 vertebrate species.</p

Supplementary figure.2

Supplementary figure.2 Identified lipid metabolites in the negative (left panel) and positive (right panel) ion modes, respectively.</p

Differential expression of genes related to glucose metabolism in domesticated pigs and wild boar

<p>Glucose metabolism is a basic biological process that shows substantial variation within and between species. Using pig as a model organism, we investigated differences in glucose metabolic genes in seven tissues from domesticated pigs (Rongchang pig and Tibetan pig, meanwhile, the Tibetan pig just as a special case of the domesticated pig under plateau condition) and wild boar. We found large differences in the expression of genes involved in multiple aspects of glucose metabolism, including genes associated with glucose transport, gluconeogenesis, and glycolysis. In addition, we identified microRNAs (miRNAs) that may be involved in the divergence of glucose metabolism in pig. A combined analysis of mRNA and miRNA expression indicated that some miRNA:mRNA pairs showed <i>ab facto</i> function in it. Our results provide a valuable resource for further determination of miRNA regulatory roles in pig glucose metabolism and reveal the divergence of glucose metabolism in pigs under domestication.</p

Hierarchical cluster analysis of the top 50 differentially expressed miRNAs (<i>P</i> < 0.05).

<p>Normalized log (base 2) data was hierarchically clustered by miRNA reads counts and is plotted as a heat map. LW1, LW2 and LW3 refer to the three biological replications of Large White pigs; MS1, MS2 and MS3 refer to the three biological replications of Meishan pigs.</p

The biological function of miR-133 during skeletal muscle development.

<p>MyoD modulate muscle proliferation by regulating expression of miR-133.</p

Top 10 unique miRNAs with the highest reads counts.

<p>Top 10 unique miRNAs with the highest reads counts.</p

The expression levels of miR-133, miR-1 and miR-206 in Large White (LW) and Meishan (MS) pigs during skeletal muscle development at 35 to 90 dpc (E35, E55 and E90).

<p>For each miRNA, the expression level of LW pigs at 35 dpc are given as a negative control and set at 1. Two-way ANOVA (<i>n</i> = 3 per breed per time point). ‘B’ and ‘T’ mean breed and time, respectively. Values are means ± SD. Different capital letters means significant difference (<i>P</i><0.05).</p