In silico identification and comparative analysis of differentially expressed genes in human and mouse tissues

BACKGROUND: Screening for differentially expressed genes on the genomic scale and comparative analysis of the expression profiles of orthologous genes between species to study gene function and regulation are becoming increasingly feasible. Expressed sequence tags (ESTs) are an excellent source of data for such studies using bioinformatic approaches because of the rich libraries and tremendous amount of data now available in the public domain. However, any large-scale EST-based bioinformatics analysis must deal with the heterogeneous, and often ambiguous, tissue and organ terms used to describe EST libraries. RESULTS: To deal with the issue of tissue source, in this work, we carefully screened and organized more than 8 million human and mouse ESTs into 157 human and 108 mouse tissue/organ categories, to which we applied an established statistic test using different thresholds of the p value to identify genes differentially expressed in different tissues. Further analysis of the tissue distribution and level of expression of human and mouse orthologous genes showed that tissue-specific orthologs tended to have more similar expression patterns than those lacking significant tissue specificity. On the other hand, a number of orthologs were found to have significant disparity in their expression profiles, hinting at novel functions, divergent regulation, or new ortholog relationships. CONCLUSION: Comprehensive statistics on the tissue-specific expression of human and mouse genes were obtained in this very large-scale, EST-based analysis. These statistical results have been organized into a database, freely accessible at our website , for easy searching of human and mouse tissue-specific genes and for investigating gene expression profiles in the context of comparative genomics. Comparative analysis showed that, although highly tissue-specific genes tend to exhibit similar expression profiles in human and mouse, there are significant exceptions, indicating that orthologous genes, while sharing basic genomic properties, could result in distinct phenotypes

Capture of CO2 from Coal-fired Power Plant with NaOH Solution in a Continuous Pilot-scale Bubble-column Scrubber

AbstractA continuous pilot-scale bubble-column scrubber with NaOH as the absorbent was used to explore the capture of CO2 gas from a coal-fired power plant. The experimental design was based on the results of previous study. The diameter of the column was 20cm and the height of the column was 2.4 m. According to the S/N ratio, parameters, including absorption rate (RA), absorption efficiency (E), overall mass-transfer coefficient (KGa) and ratio of the gas-liquid flow rate (R), were selected for Taguchi analysis to obtain optimum conditions. A total of eleven experiments were carried out to verify the optimum conditions here. The range of the gas-flow rate (Qg) and liquid-flow rate (QLT) conducted in this work were 48-192 L/min and 1.6-10 L/min, respectively. The input gas concentrations were 9-12.2%. Using a steady-state material balance with a two-film model, RA and KGa could be determined. The results showed that E, RA and KGa were in the range of 30-98%, 1.03x10-4-11.48x10-4mol/s-L and 0.018-0.058 1/s, respectively. The obtained scrubbing factors (φ) were 0.00285-0.146mol/mol-L, while R was in the range of 0.23-24.14. The dynamic behavior of the scrubber was also discussed in this study. The results could be used as a basis for commercial scale operation for the carbon capture at a power plant as well as microalgae cultivation

Acute Viral Hepatitis C-Induced Jaundice in Pregnancy

SummaryObjectiveAcute viral hepatitis C-induced jaundice in pregnancy is very rare and may be fatal. Here, we report a complicated case with acute hepatitis C-induced jaundice in pregnancy with successful managementCase ReportA 27-year-old pregnant woman, gravida 2, para 1, with gestational age of 36 weeks and 5 days, was referred to our hospital due to jaundice and elevated liver enzymes of undetermined cause. She had been suffering from general weakness, diarrhea and vomiting for 1 week, and jaundice with tea-colored urine for 3 days. At our medical center, acute viral hepatitis C-induced jaundice was suspected. Since her general condition deteriorated at 36 weeks and 6 days of gestation, we decided to induce labor. A male baby was born smoothly via the vaginal route, with birth weight 2,857 g, birth length 48.6 cm, and 1- and 5-minute Apgar scores of 7 and 9, respectively. Maternal condition improved dramatically after delivery and her serum liver enzymes and bilirubin levels gradually approached normal ranges.ConclusionMothers and fetuses with acute viral hepatitis C-induced jaundice during pregnancy are at great risk of mortality and morbidity. Timely termination may be one of the choices of treatment when fetal maturity has been reached and the maternal condition has deteriorated

TRIM28-Regulated Transposon Repression Is Required for Human Germline Competency and Not Primed or Naive Human Pluripotency.

Transition from primed to naive pluripotency is associated with dynamic changes in transposable element (TE) expression and demethylation of imprinting control regions (ICRs). In mouse, ICR methylation and TE expression are each regulated by TRIM28; however, the role of TRIM28 in humans is less clear. Here, we show that a null mutation in TRIM28 causes significant alterations in TE expression in both the naive and primed states of human pluripotency, and phenotypically this has limited effects on self-renewal, instead causing a loss of germline competency. Furthermore, we discovered that TRIM28 regulates paternal ICR methylation and chromatin accessibility in the primed state, with no effects on maternal ICRs. Taken together, our study shows that abnormal TE expression is tolerated by self-renewing human pluripotent cells, whereas germline competency is not

Perforated Appendiceal Mucinous Cystadenoma Mimicking Ruptured Appendicitis With Abscess Formation: CT Imaging Features

AbstractWe describe the imaging features of a perforated appendiceal mucinous cystadenoma in a 72-year-old woman presenting with right lower quadrant abdominal pain, mimicking ruptured appendicitis with abscess formation. Computed tomography revealed a rim-enhanced cystic lesion at the proximal appendiceal orifice, connecting with the swollen and dilated distal part of the appendix. Disruption of the appendiceal walls and peri-appendiceal fatty infiltrations were also noted. Under the impression of ruptured appendicitis with abscess formation, the patient underwent exploratory laparotomy and appendectomy. The pathologic diagnosis was perforated appendiceal mucinous cystadenoma associated with superinfection, complicated by secondary appendicitis. The patient was uneventfully discharged on the 7th hospital day. Although primary neoplasms of the appendix are uncommon, they should be considered as a predisposing factor in elderly patients manifesting with appendicitis

Ring Chromosome 7 Presenting with Intrauterine Growth Restriction and Multiple Anomalies

SummaryObjectiveRing chromosome 7 is a very rare chromosomal anomaly that may have a grave prognosis. Nevertheless, the clinical features associated with ring chromosome 7 are highly variable. Here, we report a case with ring chromosome 7 and the perinatal findings.Case ReportA 32-year-old, gravida 1, para 0, woman was referred to our hospital because of intrauterine growth restriction (IUGR) and oligohydramnios at 35 weeks of gestation. Prenatal ultrasound revealed a severe IUGR fetus presenting with multicystic kidney, hydronephrosis and oligohydramnios. At parturition, the birth weight of this male infant was 1,720 g, and a battery of anomalies were also noted, including imperforate anus, hypospadia, micropenis, right cryptorchidism, severe IUGR, multiple nevi on the forehead, shoulder and left thigh, brain atrophy, right multicystic kidney, and left mild hydronephrosis. Cytogenetic study from cord blood revealed a ring chromosome 7.ConclusionRing chromosome 7 is extremely rare and our case might be the 15th and youngest case in the medical literature. Our case had multicystic kidney and imperforate anus, which have not been reported previously. Prenatal diagnosis of ring chromosome 7 is very difficult. When fetuses present with severe IUGR, oligohydramnios and multicystic kidney, chromosomal aberrations should be kept in mind, and perinatal cytogenetic workup is warranted