Analysis of MYO-Inositol effect on spermatozoa motility, in hyper viscous ejaculates and in patients with grades II and III varicocele

The goal of this study is to evaluate MYOInositol effects on spermatozoa motility, in patients' ejaculates with severe varicocele or hyper viscosity. The study included normal viscosity ejaculate from 30 patients affected by varicocele and hyper viscosity ejaculate from 33 patients without any testicular pathologies. All selected samples showed sperm concentration > 2 million/ml and progressive motility < 32%. In both groups, the pellet obtained after centrifugation in buffered medium, was divided in two aliquots, both incubated for 15 minutes at 37°C: one with MYO-Inositol and the other one, as control, only in phosphate buffered saline (PBS). Afterwards, the sperm progressive motility was assessed using Computer Assisted Sperm Analysis (CASA system). Incubation with MYO-Inositol improved sperm progressive motility in high viscosity samples compared to control group (38.9% ± 3.0 vs 24.35% ± 2.41, respectively; p ≤ 0.0001). Conversely, no statistically significant difference was observed in total sperm progressive motility in varicocele samples compared with control group (22.7% ± 2.07 vs 26.7% ± 3.31, respectively; p = 0.085). The MYO-Inositol positive effect on spermatozoa motility may depend on the type of sperm damage: heavy structural and biochemical defects which typically affects patients with varicocele are not restored by Inositol. On the contrary, MYOInositol is able to improve sperm motility in semen samples with high viscosity, since those samples show no substantial structural sperm defects

A S.M.A.R.T. system for the seismic vulnerability mitigation of Cultural Heritages

Both assessment and mitigation of seismic vulnerability connected to cultural heritages monitoring are non-trivial issues, based on the knowledge of structural and environmental factors potential impacting the cultural heritage. A holistic approach could be suitable to provide an effective monitoring of cultural heritages within their surroundings at different spatial and temporal scales. On the one hand, the analysis about geometrical and structural properties of monuments is important to assess their state of conservation, their response to external stresses as well as anomalies related to natural and/or anthropogenic phenomena (e.g. the aging of materials, seismic stresses, vibrational modes). On the other hand, the investigation of the surrounding area is relevant to assess environmental properties and natural phenomena (e.g. landslides, earthquakes, subsidence, seismic response) as well as their related impacts on the monuments. Within such a framework, a multi-disciplinary system has been developed and here presented for the monitoring of cultural heritages for seismic vulnerability assessment and mitigation purposes*. It merges geophysical investigations and modeling, in situ measurements and multi-platforms remote sensing sensors for the non-destructive and non-invasive multi-scales monitoring of historic buildings in a seismic-prone area. In detail, the system provides: a) the long-term and the regional-scale analysis of buildings’ environment through the integration of seismogenic analysis, airborne magnetic surveys, space-borne Synthetic Aperture Radar (SAR) and multi-spectral sensors. They allow describing the sub-surface fault systems, the surface deformation processes and the land use mapping of the regional-scale area on an annual temporal span; b) the short-term and the basin-scale analysis of building’s neighborhood through geological setting and geotechnical surveys, airborne Light Detection And Radar (LiDAR) and ground-based SAR sensors. They enable assessing the site seismic effects, the built-up structural features and the surface deformation processes of the local-scale area on a monthly temporal span; c) the real- to near-real-time and building scale analysis of the heritage through proximal remotely sensing tools (e.g. terrestrial laser scanning, infrared thermal cameras and real aperture radar), combined with ambient vibration tests. They allow analyzing geometric, structural and material properties / anomalies of buildings as well as the state of conservation of structures on a real-time temporal span. The proposed approach is: Specific (it targets the cultural heritages monitoring for seismic mitigation purposes); Measurable (it provides synthetic descriptors or maps able to quantify structural and the environmental properties / anomalies / trends); Action-oriented (it provides information to plan consolidation and restoration actions for prevention activity); Relevant (it allows achieving consolidated results for cultural heritage monitoring); Time-related (it specifies when the results can be achieved). Meaningful results, obtained for the Saint Augustine Complex (XVI century) located in the historic center of the Calabrian chief town of Cosenza, are presented in terms of a web-based Geographic Information System (GIS) platform and a 3-dimensional (3D) visual software for the monitoring of environmental/urban landscapes and buildings. These tools represent the added-value products of the proposed SMART system, which allow integrating and combining multi-sensors analyses in order to support end-users involved into a cultural heritage monitoring.Copernicus MeetingsPublishedVienna | Austria | 17–22 April 20165T. Sismologia, geofisica e geologia per l'ingegneria sismic

<i>piggyBac </i>transposons expressing full-length human dystrophin enable genetic correction of dystrophic mesoangioblasts

Duchenne muscular dystrophy (DMD) is a genetic neuromuscular disorder caused by the absence of dystrophin. We developed a novel gene therapy approach based on the use of the piggyBac (PB) transposon system to deliver the coding DNA sequence (CDS) of either full-length human dystrophin (DYS: 11.1 kb) or truncated microdystrophins (MD1: 3.6 kb; MD2: 4 kb). PB transposons encoding microdystrophins were transfected in C2C12 myoblasts, yielding 65±2% MD1 and 66±2% MD2 expression in differentiated multinucleated myotubes. A hyperactive PB (hyPB) transposase was then deployed to enable transposition of the large-size PB transposon (17 kb) encoding the full-length DYS and green fluorescence protein (GFP). Stable GFP expression attaining 78±3% could be achieved in the C2C12 myoblasts that had undergone transposition. Western blot analysis demonstrated expression of the full-length human DYS protein in myotubes. Subsequently, dystrophic mesoangioblasts from a Golden Retriever muscular dystrophy dog were transfected with the large-size PB transposon resulting in 50±5% GFP-expressing cells after stable transposition. This was consistent with correction of the differentiated dystrophic mesoangioblasts following expression of full-length human DYS. These results pave the way toward a novel non-viral gene therapy approach for DMD using PB transposons underscoring their potential to deliver large therapeutic genes.status: publishe

The Monitoring of Urban Environments and Built-Up Structures in a Seismic Area: Web-Based GIS Mapping and 3D Visualization Tools for the Assessment of the Urban Resources

In this paper, a non-invasive infrastructural system called MASSIMO is presented for the monitoring and the seismic vulnerability mitigation of cultural heritages. It integrates ground-based, airborne and space-borne remote sensing tools with geophysical and in situ surveys to provide a multi-spatial (regional, urban and building scales) and multi-temporal (longterm, short-term and near-real-time scales) monitoring of test areas and buildings. The measurements are integrated through web-based Geographic Information System (GIS) and 3-dimensional visual platforms to support decision-making stakeholders involved in urban and structural requalification planning. An application of this system is presented over the Calabria region for the town of Cosenza and a test historical complex.The present work is supported and funded by the Italian Ministry of Education, University and Research (MIUR) under the research project PON01-02710 "MASSIMO" - "Monitoraggio in Area Sismica di SIstemi MOnumentali".Published9-134T. Sismologia, geofisica e geologia per l'ingegneria sismicaN/A or not JC

The MASSIMO system for the safeguarding of historic buildings in a seismic area: operationally-oriented platforms

In this paper, the non-invasive system MASSIMO is presented for the monitoring and the seismic vulnerability mitigation of the cultural heritage. It integrates ground-based, airborne and space-borne remote sensing tools with geophysical and in situ surveys to provide the multi-spatial (regional, urban and building scales) and multi-temporal (long-term, short-term, near-real-time and real-time scales) monitoring of test areas and buildings. The measurements are integrated through web-based GIS and 3D visual platforms to support decision-making stakeholders involved in urban planning and structural requalification. An application of this system is presented over the Calabria region for the town of Cosenza and a test historical complex

Phenolic Compounds and Capsaicinoids in Three Capsicum annuum Varieties: From Analytical Characterization to In Silico Hypotheses on Biological Activity

The affinity of specific phenolic compounds (PCs) and capsaicinoids (CAPs) present in three Capsicum annuum varieties (Friariello, Cayenne and Dzuljunska Sipka) to the transient receptor potential vanilloid member 1 (TRPV1) was investigated by integrating an analytic approach for the simultaneous extraction and analysis through high-performance liquid chromatography coupled with ion trap mass spectrometry (HPLC/ITMS) and UV detection (HPLC-UV) of PCs and CAPs and structural bioinformatics based on the protein modelling and molecular simulations of protein–ligand docking. Overall, a total of 35 compounds were identified in the different samples and CAPs were quantified. The highest content of total polyphenols was recorded in the pungent Dzuljunska Sipka variety (8.91 ± 0.05 gGAE/Kg DW) while the lowest was found in the non-pungent variety Friariello (3.58 ± 0.02 gGAE/Kg DW). Protein modelling generated for the first time a complete model of the homotetrameric human TRPV1, and it was used for docking simulations with the compounds detected via the analytic approach, as well as with other compounds, as an inhibitor reference. The simulations indicate that different capsaicinoids can interact with the receptor, providing details on the molecular interaction, with similar predicted binding energy values. These results offer new insights into the interaction of capsaicinoids with TRPV1 and their possible actions

ARGININE-VASOPRESSIN INDUCES DIFFERENTIATION OF SKELETAL MYOGENIC CELLS AND UP-REGULATION OF MYOGENIN AND MYF-5

The neurohypophyseal nonapeptide arginine(8)-vasopressin (AVP) induces phosphoinositide turnover and calcium and pH changes in skeletal myogenic cells in culture. In order to investigate the effect of AVP on skeletal myogenesis, we examined the effect of this hormone on proliferating mononucleated L6 myoblast cultures. Addition of AVP to the medium resulted in the formation of much larger myotubes than those formed in its absence and in a significant increase (2.2-fold) of the percentage of fusion within 3-4 days of treatment. The effect of AVP was dose dependent, in the 10 nM to 1 mu M range, and was observed also in primary cultures of mouse satellite cells. The rate of growth of L6 cells was not affected by AVP treatment. The induction of morphological differentiation by AVP correlated with an increased expression of muscle-specific gene products, such as myosin, and an increased number of acetylcholine receptor sites. The accumulation of mRNA transcripts of the acetylcholine receptor subunits was also enhanced by AVP. The mechanism involved in the myogenic action of AVP was investigated. Using AVP-related peptides and antagonists, we found that a specific chemical structure is required and that V1 receptors probably mediate the effect on myogenesis. Expression of muscle-specific transcription factor genes Myf-5 and myogenin and their products are strongly upregulated by AVP. Our findings support the hypothesis that AVP may represent a novel physiological modulator of skeletal muscle differentiation through its action on muscle regulatory genes

Analysis of MYO-Inositol effect on spermatozoa motility, in hyper viscous ejaculates and in patients with grades II and III varicocele

The goal of this study is to evaluate MYOInositol effects on spermatozoa motility, in patients’ ejaculates with severe varicocele or hyper viscosity. The study included normal viscosity ejaculate from 30 patients affected by varicocele and hyper viscosity ejaculate from 33 patients without any testicular pathologies. All selected samples showed sperm concentration &gt; 2 million/ml and progressive motility &lt; 32%. In both groups, the pellet obtained after centrifugation in buffered medium, was divided in two aliquots, both incubated for 15 minutes at 37°C: one with MYO-Inositol and the other one, as control, only in phosphate buffered saline (PBS). Afterwards, the sperm progressive motility was assessed using Computer Assisted Sperm Analysis (CASA system). Incubation with MYO-Inositol improved sperm progressive motility in high viscosity samples compared to control group (38.9% ± 3.0 vs 24.35% ± 2.41, respectively; p ≤ 0.0001). Conversely, no statistically significant difference was observed in total sperm progressive motility in varicocele samples compared with control group (22.7% ± 2.07 vs 26.7% ± 3.31, respectively; p = 0.085). The MYO-Inositol positive effect on spermatozoa motility may depend on the type of sperm damage: heavy structural and biochemical defects which typically affects patients with varicocele are not restored by Inositol. On the contrary, MYOInositol is able to improve sperm motility in semen samples with high viscosity, since those samples show no substantial structural sperm defects

OSTEORADIONECROSIS OF A MANDIBLE: A CASE REPORT OF IMPLANT-SUPPORTED REHABILITATION

The head and neck are the sixth most common sites of cancer in the world; the survival rate at 5 years from diagnosis is 60%. Surviving patients, after the critical phase of the disease, require proper rehabilitation. The treatment of oral neoplasia, such as surgery and radiotherapy, may often determine significant disability, such as impaired speech, swallowing, mastication and facial deformity, with severe consequences on the quality of life of these patients. Dental implant-based prosthodontic rehabilitation is a consolidated technique for improving the quality of life in patients who have overcome oral cancer. Implants provide stability and support for removable prostheses in oral cavities seriously deformed by surgical treatment. Moreover, mobile prostheses have the advantage of being removable, to check the health of oral tissues and intercept possible relapses of the neoplasia. On the other hand, a lack of residual bone following resection makes it difficult to place implants in an ideal position, and patients who have been submitted to radiotherapy of the head and neck are reported to have a reduced success rate. This paper presents the case of a 67-year-old woman rehabilitated with dental implant-based prosthesis after a hemimandibulectomy due to osteoradionecrosis, without bone reconstruction

Assessing soil-structure interaction during the 2016 central Italy seismic sequence (Italy): preliminary results

We used the moderate-magnitude aftershocks succeeding to the 2016 August 24th, Mw = 6.0, Amatrice (Italy) mainshok to asses, specially during an ongoing seismic sequence, the soil-structure interaction where cultural Heritage is involved. We have chosen as case study the San Giovanni Battista church (A.D. 1039)  in Acquasanta Terme town, about 20 Km northeast of Amatrice. First of all we studied the soil shaking features in order to characterize the input to the monument. Then, using the recordings in the church, we tried to figure out  how the input seismic energy is distributed over the different monument parts. Some preliminary results are shown and discussed